Activity of essential oils from spices against Staphylococcus spp. isolated from bovine mastitis

Pozzo, Marcelo Dal; Santurio, Deise Flores; Rossatto, Luana; Vargas, Agueda Castagna de; Alves, Sydney Hartz; Loreto, Érico S.; Viégas, Julio

doi:10.1590/s0102-09352011000500026

Cited by 31 publications

(19 citation statements)

References 13 publications

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“…Also in disk diffusion assay the results were not very satisfying, as it was inactive for 18 S. epidermidis isolates. Likewise, Nascimento et al 23 reported no antimicrobial properties in a disk diffusion test for rosemary EO, and Dal Pozzo et al 14 stated that this EO showed no activity against Staphylococcus spp.. However, in other studies, rosemary EO was effective against Staphylococcus spp.…”

Section: Resultsmentioning

confidence: 99%

“…Ten microliters from wells without bacterial growth were inoculated in the surface of MHA plates, in triplicate, and incubated at 37°C for 24 hours. The MBC was defined as the lowest concentration of EO that did not show bacterial growth 14 .…”

Section: Broth Microdilution Assaymentioning

confidence: 99%

“…Previous investigation of some EO antimicrobial activity against Staphylococcus spp. isolated from bovine mastitis showed that they may be considered as alternative candidates in mastitis therapy 14 .…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Essential Oils of Lamiaceae Aromatic Spices Towards Sheep mastitis-Causing Staphylococcus aureus and Staphylococcus epidermidis

Queiroga

Coelho

Arantes

et al. 2018

Journal of Essential Oil Bearing Plants

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Mastitis in ewes is responsible for massive economic losses. Antibiotics are routinely used for mastitis control but its excessive use leads to development of antibiotic resistance with deleterious outcomes both for animal and public health. Essential oils (EOs) show antibacterial proprieties and no resistance has been reported after prolonged exposure; however their efficacy depends on their chemical composition. In this study EOs chemical composition from four autochthonous aromatic herbs, from Alentejo region, southern Portugal, Calamintha nepeta subsp. nepeta, Lavandula stoechas subsp. luisieri, Rosmarinus officinalis and Thymus mastichina was accessed. EOs of R. officinalis showed predominance in monoterpene hydrocarbons (63 %) whereas EOs of T. mastichina, L. luisieri and C. nepeta were rich in oxygenated monoterpenes (71-95 %). The antimicrobial activity of selected EOs was investigated towards Staphylococcus aureus (n= 24) and Staphylococcus epidermidis isolates (n= 24) from ovine mastitic milk origin. Results of disk diffusion assay revealed that C. nepeta, L. luisieri and T. mastichina EOs are highly active against both S. aureus and S. epidermidis strains, whereas R. officinalis EO is highly active against S. aureus strains but inactive against several S. epidermidis isolates. EOs concentration causing bacterial growth inhibition ranged from 500 to 4,000 μg mL-1 in liquid microassays.

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Section: Resultsmentioning

confidence: 99%

Section: Broth Microdilution Assaymentioning

confidence: 99%

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Antimicrobial Activity of Essential Oils of Lamiaceae Aromatic Spices Towards Sheep mastitis-Causing Staphylococcus aureus and Staphylococcus epidermidis

Queiroga

Coelho

Arantes

et al. 2018

Journal of Essential Oil Bearing Plants

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“…Halos between 18 and 22 mm were observed in the culture of microorganisms demonstrating growth inhibition and the antimicrobial activity of the [22][23][24][25][26][27][28] Oregan, thyme, lippia, ginger, sage, rosemary, and basil extracts were studied by Pozzo et al 29 Antimicrobial activity tests on 32 strains of S. aureus isolated from bovine mastitis showed inhibitory activity in all tested microorganisms. 29 The evaluation of antimicrobial and antioxidant activity in the plant Ziziphus joazeiro, known as juazeiro or laranjeira do vaqueiro, using the agar diffusion technique and MIC, showed that the extract from leaves and bark have antioxidant activity and antimicrobial in 70% of the examined strains including Candida albicans, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes.…”

Section: Resultsmentioning

confidence: 99%

Determination of the antimicrobial activity in the hydroalcoholic extract of the plant Plectranthus ornatus Codd (Bilberry Chinese)

Santos¹,

Menezes²,

Rufino³

et al. 2014

Revista Médica De Minas Gerais

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Plectranthus ornatus Codd is a plant from Mediterranean and Near East countries. It is indicated for liver illnesses and digestion problems in popular medicine. It is used in the treatment for the control of gastritis, dyspepsia, heartburn, gastric discomfort, and hangover. This study aims to evaluate the antimicrobial activity in extracts of P. ornatus using 15 standardized microorganisms. Antimicrobial tests were performed on Mueller Hinton agar through the technique of wells. Broth microdilution tests were used for the determination of the minimum inhibitory concentration (MIC), and seeding on plates containing Mueller Hinton agar for the determination of the minimum microbicide concentration (MMC). The broth microdilution tests demonstrated that the extract inhibited the growth of Bacillus cereus, Streptococcus pyogenes, and Enterococcus faecalis at the concentrations of 20.31, 325, and 650 mg/mL, respectively. The microdilution tests showed growth inhibition of Saccharomyces cerevisiae at a concentration of 1,300 mg/ mL. The extract did not show inhibitory activity on the other tested strains. As a result of increased multiple resistances to antibiotics, microbial research in the development of new drugs that are economically viable and offer an effective safety margin have won space in the scientific community.

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“…The effective dose was considered 10 times the MIC [ 10 , 11 ]. Dosage was, therefore, prepared by adding 0.2 ml of DMSO to 1 ml of 12.5% sage EO solution.…”

Section: Methodsmentioning

confidence: 99%

Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis

et al. 2017

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Aim:The aims of this study were to evaluate the effects of intramammary infusion of sage (Salvia officinalis) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis.Materials and Methods:The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sage EO against Staphylococcus aureus were determined by the broth dilution method. Ewes were divided randomly into three main groups and received one of the following treatments; Group 1 (n=5): Dimethyl sulfoxide (DMSO) alone (5 ml; 0.2 ml of DMSO in 4.8 ml of saline), Group 2 (n=5): Amoxicillin alone (3 ml), and Group 3 (n=10): Sage EO (5 ml of sage EO solution [0.2 ml DMSO+1 ml EO+3.8 ml sterile saline]). All treatments were administered by intramammary infusion into each teat twice per day for 3 consecutive days. Milk samples for SCC and milk components determination and whole blood samples for hematology and serum biochemical analyses were collected before treatment (T0) and at 24 (T24) and 48 (T48) h after the last treatment.Results:The MIC and MBC of sage EO against S. aureus were 12.5% and 6.1%, respectively. SCC was decreased significantly (p<0.05) at T24 and T48 h in sage EO and amoxicillin treated groups. Milk fat and lactose were increased significantly (p<0.05) in sage EO and amoxicillin treated ewes while no significant changes were observed in the percentages of solids-not-fat, protein and total solids. No significant effects of sage EO treatment on any of the hematology or serum biochemical parameters were observed. There were no local or systemic side effects observed in any of the treated ewes. However, further clinical trials are warranted to determine safety and possible withdrawal times in milk before its recommendation for use in organic operations.Conclusion:In this study, the intramammary infusion of sage EO to ewes affected with subclinical mastitis resulted in a significant decrease in SCC 24 h and 48 h posttreatment. In addition, milk fat and lactose were increased in animals that received the EO as well as in those treated with the antibiotic.

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Activity of essential oils from spices against Staphylococcus spp. isolated from bovine mastitis

Cited by 31 publications

References 13 publications

Antimicrobial Activity of Essential Oils of Lamiaceae Aromatic Spices Towards Sheep mastitis-Causing Staphylococcus aureus and Staphylococcus epidermidis

Antimicrobial Activity of Essential Oils of Lamiaceae Aromatic Spices Towards Sheep mastitis-Causing Staphylococcus aureus and Staphylococcus epidermidis

Determination of the antimicrobial activity in the hydroalcoholic extract of the plant Plectranthus ornatus Codd (Bilberry Chinese)

Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis

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