Binding patterns of seminal plasma plasma proteins on bovine epididymal and ejaculated sperm membrane

Souza, Carlos Eduardo A.; Moura, Arlindo A.; Lima-Souza, A. C.; Killian, Gary J.

doi:10.1590/s0102-09352011000300001

Cited by 8 publications

(12 citation statements)

References 23 publications

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“…Moreover, in Tankwa goats the fertility-associated antigen showed a positive relationship with rapid velocity, VCL, VAP, sperm cell vitality and normal sperm cells. The results of the current study agree with those of the literature, as it has been indicated that proteins have a good relationship with semen quality and male fertility in other species (Ding et al, 2007;Souza et al, 2011). As a result, proteins are regarded as a valuable male fertility marker in conjunction with semen parameters in other species (Dawson et al, 2002;Dyck et al, 2011).…”

Section: Discussionsupporting

confidence: 90%

Characterization of seminal plasma constituents of unimproved indigenous and Tankwa goats

Ramukhithi¹,

Nephawe²,

Lehloenya³

et al. 2019

SA J. An. Sci.

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The aim of the study was to characterize and compare seminal plasma constituents of unimproved indigenous and Tankwa goats. In this study, unimproved indigenous (11) and Tankwa (11) goats were used. Their average age was 3 ± 0.8 years and average bodyweights were 35.5 ± 2.1 kg and 59.8 ± 1.9 kg, respectively. Semen samples were collected with an electro ejaculator, and evaluated for macroscopic and microscopic semen characteristics and seminal plasma constituents. Both unimproved indigenous and Tankwa goats had normal semen characteristics for goats, with the exception of sperm cell concentration. However, Tankwa goats had higher straight-line velocity and average path velocity compared with unimproved indigenous goats. Unimproved indigenous goats had higher intact acrosome than Tankwa goats. Lipocalin-type prostaglandin-D synthase was one of the constituents that were detected, but it was below the detection limit of 0.5 mmol/L. Moreover, the semen of unimproved indigenous and Tankwa goats had the same levels of fertility-associated antigen, lactate dehydrogenase, sodium, potassium, magnesium, calcium, glucose, cholesterol, triglyceride, lipids and urea. In conclusion, unimproved indigenous and Tankwa goats had the same seminal plasma constituents. These constituents seem to be optimal for the sperm cells, as they resulted in acceptable semen characteristics, except for sperm cell concentration, which was low. Moreover, acceptable standards need to be set for all seminal plasma constituents, as they are not currently known.

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Section: Discussionsupporting

confidence: 90%

Characterization of seminal plasma constituents of unimproved indigenous and Tankwa goats

Ramukhithi¹,

Nephawe²,

Lehloenya³

et al. 2019

SA J. An. Sci.

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“…Although several proteins appeared common to both cauda epididymal and vesicular fluid, such as transferrin, albumin and clusterin, others became visible only in CEF gels of the present study, such as α-enolase and prostaglandin D synthase (PGDS). PGDS binds to the acrosomal cap of sperm from both the cauda epididymis and ejaculates [90] and its expression in the seminal plasma is related to bull fertility [7]. As a hypothesis, PGDS would act as a carrier of lipophilic components (such as retinol and testosterone) in the epididymis or across the epididymal-blood barrier [91], but knowledge of the exact mechanism by which PGDS functions in male reproduction is incomplete.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of the reproductive tract fluids from tropically-adapted Santa Ines rams

Souza

Rêgo

Lobo

et al. 2012

Journal of Proteomics

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“…O seu papel na fertilização, especificamente na capacitação espermática, é conferido pela sua ligação ao grupo colina de fosfolipídios presente na membrana de espermatozoides (Desnoyers & Manjunath, 1992), lipoproteínas de alta densidade (HDL) e glicosaminoglicanos como a heparina (GAG) presentes fluido folicular e tubário (Thérien et al, 1995;2001). Ao investigar por imunocitoquímica e microscopia confocal a distribuição topográfica da ligação de proteínas seminais à membrana de espermatozoides bovinos epididimários e ejaculados, Souza et al (2011) concluiu que PGDS, BSP-A3 e NUC2 interagem diretamente com espermatozoides bovinos, e mostrou distribuição topográfica específica; a BSP-A3 ligou-se a todas as regiões estudadas, de forma mais intensa na peça intermediária e acrossoma. Estes achados permitem melhor compreensão sobre o papel desempenhado por essas proteínas na regulação da função espermática e da fertilidade.…”

Section: Marcadores Proteicos No Sêmen Caprino E De Outras Espécies Aunclassified

Two-dimensional electrophoresis and mass spectrometry as proteomic tools applied to the definition of protein markers associated with reproductive efficiency goats

Bezerra

Martins

Barroso

et al. 2013

AVB

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RESUMO -O crescimento e desenvolvimento do rebanho caprino no Nordeste são observados com o aumento na produção da pecuária do Brasil. Esse aumento é reflexo, a princípio, das maiores exigências do mercado consumidor por produtos de melhor qualidade obtidos a partir de rebanhos de alto padrão zootécnico. As pesquisas atuais ilustram a necessidade de dispor de biomarcadores que auxiliem a indicação do potencial reprodutivo dos animais, uma vez que isso não pode ser expresso apenas com o exame andrológico. A avaliação da expressão das proteínas, tomando-as como biomarcadores, é análise potencial uma vez que estas, dentre os constituintes do plasma seminal, são encontradas em maior quantidade na forma de complexos associados, desempenhando papel crucial em todos os processos relacionados à capacidade fecundante dos espermatozoides. Essa análise é realizada por métodos de separação e detecção simultânea de proteínas utilizando técnicas como a eletroforese bidimensional (2DE) ou cromatografias, acoplados a métodos cada vez mais eficientes e sensíveis de identificação e quantificação de níveis de expressão de proteínas por espectrometria de massas. O objetivo desta revisão é abordar sobre a técnica de eletroforese bidimensional associada à espectrometria de massa como ferramenta na análise da expressão de proteínas dentro do campo da proteômica.Palavras-Chave: proteínas, plasma seminal, eletroforese 2DE, espectrometria de massa.ABSTRACT -The growth and development of goat herds in the northeast are being observed due to the increase in livestock production in Brazil. This increase reflects demands of the consumer market for high quality product, which are obtained from flocks of high standard zootechnics. Current research illustrates the need for biomarkers that indicate an animal´s reproductive potential, since this cannot be expressed solely with andrologic evaluation. For this reason, the expression of the proteins as biomarkers is a potential analysis. The proteins from the seminal plasma constituents are found in larger amounts in the form of associated complexes, playing a crucial role in all processes related to the fertilizing capacity of sperm. This analysis is performed by separation methods and simultaneous detection of proteins using techniques such as two-dimensional electrophoresis (2DE) or chromatography. These techniques are coupled with methods to identify and quantify expression levels of proteins by mass spectrometry that are increasingly efficient and sensitive. The aim of this review was to discuss the technique of two-dimensional electrophoresis combined with mass spectrometry as a tool in the analysis of protein expression within the field of proteomics.

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Binding patterns of seminal plasma plasma proteins on bovine epididymal and ejaculated sperm membrane

Cited by 8 publications

References 23 publications

Characterization of seminal plasma constituents of unimproved indigenous and Tankwa goats

Characterization of seminal plasma constituents of unimproved indigenous and Tankwa goats

Proteomic analysis of the reproductive tract fluids from tropically-adapted Santa Ines rams

Two-dimensional electrophoresis and mass spectrometry as proteomic tools applied to the definition of protein markers associated with reproductive efficiency goats

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