Cryotop and development of vitrified immature bovine oocytes

Hajarian, Hadi; Wahid, H.; Rosnina, Y.; Daliri, Morteza; Dashtizad, Mojtaba; Karamishabankareh, Hamed; Mazni, O. Abas

doi:10.1590/s0102-09352011000100011

Cited by 5 publications

(2 citation statements)

References 17 publications

(10 reference statements)

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“…Finally, COCs were washed (2 times) in BS free from sucrose for 5 min to remove the intracellular cryoprotectants effects (Hajarian et al, 2011), In vitro maturation:…”

Section: Vitrification Thawing and Evaluation Of Oocyte Viabilitymentioning

confidence: 99%

Buffalo Oocytes Maturation In Vitro as Affected by Vitrification of Whole Ovaries or Oocytes

Nagy¹,

Abo-Farw²,

El‐Ratel³

et al. 2017

Journal of Animal and Poultry Production

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Aim of this paper is to find the possibility of in vitro maturation of buffalo oocytes recovered from vitrified whole ovaries. Ovaries from slaughtered buffaloes (n=400) were collected; out of these ovaries, 150 were fresh and 250 buffalo ovaries were vitrified and thawed. Number of all visible follicles was recorded on fresh ovaries and on each ovarian surface pre-and post vitrification, then oocytes recovery rate was calculated in fresh or vitrified ovaries. Oocytes were recovered by aspiration. From the recovered oocytes from fresh ovaries, COCs were vitrified by straw cryodevice. Post-thawing, morphologically normal oocytes from vitrified or fresh ovaries were vitrified. Results showed that numbers of total and normal follicles, and total and normal oocytes per ovary were significantly higher in fresh than in vitrified ovaries. Total number of abnormal follicles showed significantly (P<0.01) an opposite trend, while, the difference in number of abnormal oocytes/ovary was not significant. Oocytes recovered from fresh ovaries showed significantly higher recovery and normality rates than those recovered from vitrified ovaries. Percentage of compact and expanded oocytes was significantly higher, while percentage of denuded and partial denuded oocytes was significantly lower when oocytes were recovered from fresh than from vitrified ovaries. Maturation rate (MIIoocytes percent) was higher (P<0.05) when oocytes were recovered from fresh than from vitrified ovaries and those vitrified after recovery (62.50% vs. 35.90 and 27.50%, respectively). In conclusion, vitrification of the whole buffalo ovaries is a positive tool for genetic sources cryopreservation in term of beneficial effects on in vitro maturation of oocytes when compare with those directly vitrified after recovery from fresh ovaries.

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“…Finally, COCs were washed (2 times) in BS free from sucrose for 5 min to remove the intracellular cryoprotectants effects (Hajarian et al, 2011), In vitro maturation:…”

Section: Vitrification Thawing and Evaluation Of Oocyte Viabilitymentioning

confidence: 99%

Buffalo Oocytes Maturation In Vitro as Affected by Vitrification of Whole Ovaries or Oocytes

Nagy¹,

Abo-Farw²,

El‐Ratel³

et al. 2017

Journal of Animal and Poultry Production

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“…After storage for at least 2 weeks in LN2, all types of vitrified oocytes(immature or mature) were warmed by holding the OPS for 6 s in air and then agitating them in water bath at 20 °C for at least 10 s. The contents of OPS were expelled into Petri dish. To remove of intracellular cryoprotectants effects, oocytes were transferred in BM plus 0.25M sucrose for 5 min and then transferred to buffer solution (BS) plus 0.125M sucrose solution for 5 min and finally, the oocytes were washed twice in BS without sucrose for 5 min according to Hajarian et al (2011) with minor modifications.…”

Section: Thawing and Evaluation Of Oocyte Viabilitymentioning

confidence: 99%

In Vitro Fertilization of Vitrified Immature or Mature Oocytes as Affected by Mechanical or Natural Denudation

Gabr

Nowier

2016

Egyptian Journal of Nutrition and Feeds

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he current study aimed to evaluate the role of cumulus-corona radiate complex of immature or in vitro matured bovine oocytes on their vitrification, in vitro fertilization and embryo development. Bovine ovaries were collected from an abattoir and follicular oocytes (3-8 mm in diameter) were aspirated. Oocytes were examined and classified into cumulus oocytes-complexes (COCs), natural denuded oocytes (NDOs) and mechanically denuded oocytes (MDOs). Natural COCs were mechanically denuded by repeated pipetting in phosphate buffer solution (PBS). All types of oocytes were cryopreserved by vitrification as immature or in vitro matured for 24 h prior to vitrification by open-pulled straw cryodevice. After at least 2 weeks of vitrification, all types of vitrified oocytes were evaluated for survival and viability (Normal and abnormal oocytes). Morphologically normal immature oocytes were matured and fertilized in vitro, while morphologically normal mature oocytes were directly fertilized in vitro for determination of cleavage rate (CR). After co-culture of cleaved oocytes for 7 days, rate of morula (MPR) and/or blastocysts (BPR) production was recorded. Results show that survival (SR) and normality (NR) rates were the highest for CCOs (84.47 and 81.73%), followed by MDOs (73.28 and 62.0%), and the lowest for NDOs (60.71 and 54.39%), respectively (P<0.05). SR was higher (P<0.05) for mature than immature oocytes (78.03 vs. 67.60%), while NR was nearly similar for both. CR, MPR and BPR were higher (P<0.05) for CCOs (45.28, 18.05 and 15.28%) than those of MDOs (29.72, 6.06 and 3.03%) and NDOs (25.24, 7.69 and 7.69%), respectively. CR was higher (P<0.05) in mature than in immature oocytes (41.93 vs. 29.36%), while MPR and BPR were nearly similar in both. Based on the obtained results, cumulus cell surrounding cumulus oocytes-complexes play a vital role for survival of bovine oocytes during vitrification and successful in vitro maturation and fertilization as well as embryonic development to morula and blastocyst stages.

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Methodological approaches for vitrification of bovine oocytes

Dujíčková

Makarevich

Olexíková

et al. 2020

Zygote

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Summary Numerous factors affect vitrification success and post-thaw development of oocytes after in vitro fertilization. Therefore, elaboration of an optimal methodology ensuring higher cryotolerance of oocytes and subsequent blastocyst yield is still of great interest. This paper describes and evaluates critical factors affecting the success of oocyte vitrification. In particular, an appropriate oocyte stage such as maturation status (germinal vesicle stage, metaphase II stage), presence/absence of cumulus cells before vitrification, and the effect of follicle size, as well as different culture systems and media for in vitro production of embryos, the types and concentrations of cryoprotectants, and cooling and warming rates at vitrification are considered. Special attention is paid to various cryocarriers used for low-volume vitrification, which ensures safe storage of oocytes/embryos in liquid nitrogen and their successful post-thaw recovery. At the end, we focussed on how age of oocyte donors (heifers, cows) influences post-thaw development. This review summarizes results of recently published studies describing different methodologies of cryopreservation and post-thaw oocyte development with the main focus on vitrification of bovine oocytes.

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Cryotop and development of vitrified immature bovine oocytes

Cited by 5 publications

References 17 publications

Buffalo Oocytes Maturation In Vitro as Affected by Vitrification of Whole Ovaries or Oocytes

Buffalo Oocytes Maturation In Vitro as Affected by Vitrification of Whole Ovaries or Oocytes

In Vitro Fertilization of Vitrified Immature or Mature Oocytes as Affected by Mechanical or Natural Denudation

Methodological approaches for vitrification of bovine oocytes

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