Infecção experimental por Salmonella enterica subespécie enterica sorotipo Panama e tentativa de transmissão nasonasal em leitões desmamados

Oliveira, Luís Guilherme de; Carvalho, Luis Felipe das Chagas e Silva de; Masson, Gabriela Abrahão; Feliciano, Marcus Antônio Rossi

doi:10.1590/s0102-09352010000600007

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…The inoculum was prepared as recommended by Wood et al (1991) and Oliveira et al (2010) from a Salmonella Typhimurium strain isolated from porcine feces and naturally resistant to nalidixic acid.…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…Organ fragment samples were pre-enriched in buffered peptone water (CM0509, Oxoid) at a ratio of 1:10 before the selective enrichment procedure. The samples were homogenized (Sample Homogenizer MA440, Marconi, Piracicaba, SP) and incubated for 24 h at 37 o C. After this pre-enrichment step, 1 mL aliquots were transferred to tubes containing 9 mL of selenite cystine (SC) broth and incubated for 24 h to 37 o C. Subsequently, 10 µL of SC broth was plated in modified-brilliant green agar (CM0329, Oxoid) with 50 µg/mL of nalidixic acid and incubated for 24 h at 37 o C. Typical colonies were tested on lysine iron agar (LIA) (CM0381, Oxoid) and triple sugar iron agar (TSI) (CM0277, Oxoid) (Oliveira et al, 2010). Suspicious colonies were identified as Salmonella by slide agglutination with polyvalent Salmonella O antisera and then with Salmonella group B antisera (Probac of Brazil, São Paulo, SP, Brazil).…”

Section: Microbiological Examinationmentioning

confidence: 99%

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Use of organic acids to reduce Salmonella Typhimurium excretion in swine

Silva

Moura

Sanches

et al. 2023

Braz. J. Vet. Res. Anim. Sci.

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The use of antimicrobials as growth promoters and disease prevention is being constantly reduced in several animal production systems, including in the swine industry. Therefore, this study aimed to evaluate the effectiveness of using acidifiers to control Salmonella Typhimurium in 65-day-old pigs by detecting the pathogen in organs at euthanasia. For this, 24 piglets were divided into two experimental groups consisting of 12 piglets each. An untreated control group (G1) and a treatment group (G2) received a liquid organic acidifier in the drinking water for 10 days (D-5 to D5). Five days after the start of treatment (D0), all piglets were challenged with 106 CFU of Salmonella Typhimurium and assessed for 12 days (D12). Every three days (D3, D6, D9, and D12), three animals from each experimental group were euthanized and then submitted for necropsy. Samples from the intestines (ileum, cecum, mesenteric lymph nodes, and ileocolic lymph nodes), liver, spleen, and lungs were collected to isolate Salmonella. The results show that, numerically, Salmonella isolation in the organs of G2 was lower than in G1 and that the number of positive cecum samples in G1 (66.7%; 8/12) was statistically different from the number of positive models in G2 (16.7%; 2/12), with a reduction of 28.6% of the total cecum positive samples in the treated group compared to the control. Therefore, it was observed that the liquid organic acidifier product could reduce the colonization of organs by Salmonella Typhimurium.

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Section: Inoculum Preparationmentioning

confidence: 99%

Section: Microbiological Examinationmentioning

confidence: 99%

Use of organic acids to reduce Salmonella Typhimurium excretion in swine

Silva

Moura

Sanches

et al. 2023

Braz. J. Vet. Res. Anim. Sci.

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“…A Salmonelose é uma doença de importância mundial e zoonose de extrema importância em saúde pública. Os ovos, a carne de aves e seus derivados e a carne suína são as principais fontes de contaminação da samonelose humana (OLIVEIRA, 2008).…”

Section: Salmonella Spunclassified

“…A prevalência de Salmonella sp. na produção animal deve ser controlada, pois a taxa de contaminação de carcaças está diretamente ligada à freqüência nos animais de abate (OLIVEIRA, 2008).…”

Section: Salmonella Spunclassified

Efeito da combinação de probióticos na dieta de leitões desafiados com Salmonella Typhimurium

Parazzi¹

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Primeiramente a Deus, Nossa Senhora Aparecida e "meu" Santo Expedito por tudo!!! Agradeço a minha família, que me apoiou em tudo sempre, proporcionando a realização dos meus sonhos. Ao Marcão, que esteve sempre presente e pronto pra ajudar! Agradeço ao professor Dr. Aníbal de Sant'Anna Moretti, por me orientar, ensinar, acreditar em mim e pela amizade! À FAPESP pelo auxílio à pesquisa e ao CNPq pela bolsa concedida. À professora Dra. Simone por sempre me ajudar, orientar, e pela amizade! À "filhinha" Esther, que foi meu braço direito, esquerdo e as duas pernas no experimento! Sempre dedicada e amiga! Forever! E em especial, aos pais dela, Marisa e João Carlos que ajudaram em momentos cruciais! Ao Fabinho que sempre nos ajudou, resolveu os pepinos, perdendo até feriados para pesar leitões, sempre dedicado! Ao Emu, que me ajudou muito e me ensinou sempre de boa vontade. À professora Dra. Andrea Micke Moreno s empre dedicada e prestativa e a professora Dra. Lilian da patologia. Ao Dr. Abrão, sempre ensinando e resolvendo os pepinos. À Alessandra da secretaria da graduação, sempre resolvendo nossos galhos com carinho. Ao João Paulo e ao Zeca pela colaboração. Ao professor Dr. Paulo Mazza que me ajudou demais com a estatística e me deu bons conselhos. E a Carol Tobias por me dar várias dicas! Ao professor Dr. Augusto Gameiro pela orientação e ajuda.

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