Histopatologia das lâminas do casco de equinos com laminite aguda induzida e tratados com ketoprofeno, fenilbutazona e flunixin meglumine

Abstract

Avaliaram-se as alterações histológicas do tecido laminar, obtido por biopsia, em 20 equinos portadores de laminite induzida por sobrecarga de carboidratos e tratados com ketoprofeno, fenilbutazona ou flunixin meglumine. A biopsia foi colhida dos dígitos torácicos 72 horas após a indução. Os achados histológicos foram comparados com os achados de amostras de equinos isentos de laminite. Infiltrado inflamatório neutrofílico foi observado em 80%, congestão em 50%, hemorragia em 35% e hiperplasia na túnica íntima das arteríolas das lâminas dérmicas primárias em 15% das amostras. As taxas de microtrombos e coágulos foram 15% e 20%, respectivamente. Estes achados parecem decorrer dos distúrbios circulatórios que ocasionaram edema, congestão e hiperemia, seguidos de degeneração. Em 70% das análises realizadas nos animais tratados, as lesões histológicas foram inferiores aos graus de claudicação observados. Conclui-se que a biopsia de tecido laminar digital de equinos é viável, os artefatos decorrentes da técnica de biopsia não prejudicam a análise histológica das amostras e os anti-inflamatórios não esteroidais não são capazes de evitar as lesões laminares quando administrados após o início da sintomatologia clínica de laminite.

Evaluation of the Integrity of Horse Hoof Dermal and Epidermal Tissues Collected by Dorsal Transmural Access

“…The goal of this procedure was to preserve the sample's integrity, similar to that of the other investigators who stopped drilling when the white layer was evident and the wall was soft to the touch [6,11]. However, other authors stopped drilling only after the bottom of the hole was pink [3]; this color indicates the presence of blood vessels and marks the end of the stratum medium and the start of the internal extract.…”

“…With regard to performing a hoof biopsy to assess laminar tissue, there are only six reports of this procedure, of which five were in horses [6,7,[9][10][11] and one was in cattle [3]. The first step to obtain a laminar tissue sample was to drill a cone-shaped hole in the hoof wall, from which the external extract and the rigid part of the stratum medium were removed using a grinding stone attached to a rotary drill.…”

“…The first step to obtain a laminar tissue sample was to drill a cone-shaped hole in the hoof wall, from which the external extract and the rigid part of the stratum medium were removed using a grinding stone attached to a rotary drill. This method of opening a hole in the hoof wall to access the laminar tissue using a rotary drill [6,7,9], a rasp, and hoof knife was also used [11].…”

“…In the present study, we cut into the laminae to the level of the distal phalanx using a disposable size 11 scalpel blade to form a cube, as described previously [6]. An alternative method for reaching the distal phalanx is to make cuts using a biopsy punch [3,7,10,11], but this method complicates the detachment of the tissue from the distal phalanx.…”

Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist

“…Biopsy has been used to histologically evaluate equine lamella by other authors (Hanly et al 2009, Paes Leme et al 2010, Visser & Pollitt 2011, Gravena et al 2012, including serial collection (Hanly et al 2009, Visser & Pollitt 2011. In the present study, a new hoof biopsy technique was used.…”