Caracterização genética de Brycon orbignyanus utilizando o sistema seminatural

Lopera-Barrero, Nelson Maurício; Vargas, Lauro; Sirol, R.N.; Ribeiro, Ricardo Pereira; Povh, Jayme Aparecido; Mangolin, Claudete Aparecida

doi:10.1590/s0102-09352010000100025

Cited by 12 publications

(4 citation statements)

References 16 publications

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“…According to Telles et al (2001), the number of fragments obtained is more important than the primers used. This suggests that 50 fragments are sufficient to obtain reliable genetic variability rates, or rather, the number of fragments obtained in current analysis revealed a reliable estimate of genetic variability of broodstocks, Similar results were obtained by Lopera-Barrero et al (2010b) who observed 90 fragments with nine primers when they characterized the genetic variability of Brycon orbignyanus stocks. Similarly, Lopes et al (2009) reported 100 fragments with 10 primers when calculating the genetic variability of C. macropomum stocks.…”

Section: Variability and Genetic Structuresupporting

confidence: 86%

Genetic characteristics of Tambaqui broodstocks in the state of Rondônia, Brazil: implications on production and conservation

Ribeiro

Rodríguez-Rodríguez

Resende

et al. 2016

SCA

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In recent years, the genetic monitoring of broodstocks in fish farming has been greatly highlighted due to its importance in the management and improvement of their production and conservation. Current study evaluates the genetic diversity of four Tambaqui broodstocks (Colossoma macropomum) in the state of Rondônia (Brazil) and discusses activities towards the species´s correct production management and conservation. Nine primers were employed to analyze 94 specimens from four fish farms in the municipalities of Ji-Paraná (JP), Ouro Preto do Oeste (OP), Presidente Médici (PM) and Rolim de Moura (RM). Differences in the frequency of 38 fragments, with an exclusive fragment in JP and OP, were reported. High polymorphism (52.40 to 64.60%) and Shannon Index (0.313 to 0.382) rates were observed. The analysis of molecular variance (AMOVA) demonstrated that most variation is within each stock. The identity and genetic distance between the groups ranged between 0.927 and 0.954 and between 0.047 and 0.076 respectively, with shortest distance between the OPxPM and JPxRM groups. Genetic differentiation ranged from moderate to high (Fst = 0.081 to 0.179) and the number of migrants per generation was moderate (Nm = 3.83 to 6.24). As a rule, stocks showed high genetic variability and moderate / high differentiation and genetic distance between them. The results allowed direct conservation programs and increase the productivity of this species in fish farming through breeding programs. ResumoNos últimos anos, o monitoramento genético de estoques na piscicultura tem recebido grande destaque devido a sua importância no gerenciamento e melhora da sua produção e conservação. Dessa forma, o objetivo deste estudo foi avaliar de forma inédita a diversidade genética de quatro estoques de reprodutores Tambaqui (Colossoma macropomum) do estado de Rondônia (Brasil) e através dela, discutir ações que contribuam com o correto manejo produtivo e conservação dessa espécie. Utilizaram-se nove iniciadores para analisar 94 indivíduos de quatro pisciculturas dos municípios Ji-Paraná (JP), Ouro Preto do Oeste (OP), Presidente Medici (PM) e Rolim de Moura (RM). Foram encontradas diferenças nas frequências de 38 fragmentos, com um fragmento exclusivo em JP e OP. Altos valores de polimorfismo (52,40 a 64,60%) e índice de Shannon (0,313 a 0,382) foram observados. A análise de variância molecular (AMOVA) demostrou que a maior parte da variação está dentro de cada estoque. A identidade e distância genética entre os agrupamentos variou de 0,927 a 0,954 e 0,047 a 0,076, respectivamente, com menor distância entre os agrupamentos OPxPM e JPxRM. A diferenciação genética variou de moderada a alta (Fst = 0,081 a 0,179) e o número de migrantes por geração foi moderado (Nm = 3,83 a 6,24). De forma geral, os estoques apresentaram alta variabilidade genética e moderada/alta diferenciação e distancia genética entre eles. Os resultados permitirão direcionar programas de conservação e aumentar a produtividade dessa espécie na piscicultura através de program...

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Section: Variability and Genetic Structuresupporting

confidence: 86%

Genetic characteristics of Tambaqui broodstocks in the state of Rondônia, Brazil: implications on production and conservation

Ribeiro

Rodríguez-Rodríguez

Resende

et al. 2016

SCA

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“…Lopera‐Barrero et al. () studying piracanjuba seminatural reproduction method demonstrated results achieving 100% of survival. However, even without significant mortality rates, the occurrence of acute stress may predispose fish pathologies due to immunosuppression caused by stress (Barton and Iwama, ).…”

Section: Resultsmentioning

confidence: 99%

Physiological and biochemical changes of females of Piracanjuba, subjected to induced reproduction

Zanoni¹,

Costa²,

Carvalho³

et al. 2015

Animal Physiology Nutrition

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The aim of this experiment was to compare the level of fish female stress during induced reproduction with pituitary extract by two different methods, natural and semiextruded. The reproductive efficiency was 62.5% in the seminatural treatment and 100% in the extruded. Obtained egg volume was 5200 ml and 4000 ml, for seminatural and extruded treatments respectively. The mean number of eggs was 46.7 for the seminatural and 52.0 and for the extruded treatment. The percentage of viable eggs was, respectively, 87.2% and 8.17% for the natural treatment and extruded semimethods. Blood samples were collected to quantify cortisol and glucose levels, as well as red cell series and lymphocyte count. Fishes submitted to induction procedures showed elevated cortisol and glucose levels, compared to the control animals. The results for haematocrit, haemoglobin concentration and red blood cell count showed no significant differences among groups. Significant differences found in the number of lymphocytes and monocytes suggest the general adaptation syndrome. Our results suggest the reproductive induction process with extrusion of gametes as a more stressful method than seminatural reproduction process.

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“…This procedure is traditional and widespread in the reproducetive sector. However, recent studies with neotropical fish species have demonstrated that natural spawning, which has no human interference during gamete release, reduces fish mortality and increases the egg fertilization rate [3].…”

Section: Introductionmentioning

confidence: 99%

Artificial reproduction of neotropical fish: Extrusion or natural spawning?

Reynalte‐Tataje¹,

Lopes²,

Ávila-Simas³

et al. 2013

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In captive conditions, most neotropical migratory species do not have the necessary incentive to complete gonadal maturation and spawning, which require induction techniques, usually with the use of hormones. Numerous studies have therefore focused on finding an effective hormonal treatment to induce reproduction for each species. A standard treatment was successfully developed for most of these species, which consists of two doses of Carp Pituitary Extract (CPE), totaling 5.5 mgCPE/kg of fish. However, the best strategy for the subsequent fertilization of gametes is still unclear. This study was conducted with five species of commercial interest that do not reproduce naturally in captivity: pacu Piaractus mesopotamicus, piracanjuba Brycon orbignyanus, curimbatá Prochilodus lineatus, dourado Salminus brasiliensis and piau?u Leporinus macrocephalus. Ninety-nine broodstocks were used, consisting of males and females of the five species to compare two techniques for spawning and fertilization: extrusion followed by the dry method and natural spawning. All fish were induced with two hormone doses, i.e., 0.5 and 5.0 mgCPE/kg, and were subjected to one of the two types of fertilization. The results were compared using the fertilization rate, number of oocytes produced, relative fecundity and the broodstocks' survival rate on days following spawning. All species responded positively to spawning for extrusion, and only S. brasiliensis did not spawn through the natural spawning method. The natural spawning technique provided a higher fertilization rate of eggs and a greater broodstock survival rate (P < 0.05) for all species, except S. brasiliensis. It was concluded that the natural spawning technique can increase the production of viable eggs and reduce the mortality of species during breeding, except for S. brasiliensis.

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Caracterização genética de Brycon orbignyanus utilizando o sistema seminatural

Cited by 12 publications

References 16 publications

Genetic characteristics of Tambaqui broodstocks in the state of Rondônia, Brazil: implications on production and conservation

Genetic characteristics of Tambaqui broodstocks in the state of Rondônia, Brazil: implications on production and conservation

Physiological and biochemical changes of females of Piracanjuba, subjected to induced reproduction

Artificial reproduction of neotropical fish: Extrusion or natural spawning?

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