Variação da contagem de células somáticas em vacas leiteiras de acordo com patógenos da mastite

Souza, Guilherme Nunes de; Brito, J. R. F.; Moreira, É. C.; Brito, Maria Aparecida Vasconcelos Paiva; Silva, M.V.G.B.

doi:10.1590/s0102-09352009000500001

Cited by 41 publications

(43 citation statements)

References 9 publications

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“…This pathogen has been regarded as a minor pathogen with limited clinical significance (Oliveira et al 2013), probably due at least in part to the modulation of innate immunity (i.e., toll-like receptor 2) in an attempt to control inflammation and tissue injury (Blagitz et al 2015). However, major pathogens, including S. aureus, S. agalactiae and Streptococcus uberis, led to a significant augmentation of the milk SCC in the present study, as previously described (Djabri et al 2002, Souza et al 2009, Botaro et al 2015.…”

Section: Discussionsupporting

confidence: 73%

Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples

et al. 2016

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The most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was designed to evaluate a set of criteria to classify the infectious status of an udder at the quarter (single and duplicate milk samples) and cow (composite milk sample) levels, and to compare the infectious status with somatic cell counts (SCCs) of the samples. Here, the SCC thresholds determined by receiver operating characteristic curve analysis had a higher Youden index using mammary quarter duplicate milk samples as the gold standard for testing compared with single quarter and composite milk samples, especially for samples for which at least one of the duplicates was microbiologically positive, regardless of the mastitis pathogen isolated. The kappa coefficient for bacteriological results of the single quarter milk samples (single S1 and S2) was 0.85+0.019, indicating that single quarter milk sampling can be useful in mastitis control programs. Therefore, the use of composite milk samples to detect mastitis pathogens may be limited to the detection of major pathogens, given their predictive values. Thus, our findings suggest that the milk SCCs and microbiological examinations, although regarded as the most reliable indicators of ongoing mastitis, should be used in an integrated manner in mastitis control programs. Furthermore, the accuracy of single, duplicate and composite microbiological analyses to diagnosis mastitis should be considered for its implications in mastitis control strategies.INDEX TERMS: Somatic cell count, mastitis, intramammary infection, sensitivity, specificity, udder health status, dairy cow.

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Section: Discussionsupporting

confidence: 73%

Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples

et al. 2016

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“…The major pathogens cause the greatest SCC increases (Staphylococcus aureus, Streptococcus agalactiae, coliforms, and Streptococcus spp. other than S. agalactiae), and the minor pathogens (Corynebacterium bovis and coagulase-negative staphylococci) usually cause only a moderate increase in SCC (SOUZA et al, 2009). Metaanalysis showed the effects associated with intramammary infection (IMI) at the quarter level by a bacterium or a group of bacteria on SCC.…”

Section: Este Estudo Foi Realizado Com Objetivo De Estabelecer a Relamentioning

confidence: 99%

Relationship between total bacteria counts and somatic cell counts from mammary quarters infected by mastitis pathogens

et al. 2012

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This study was conducted to establish the relationship between somatic cell count (SCC) and bacterial shedding from mammary quarters according to mastitis pathogens. Milk samples from 638 mammary quarters were examined for mastitis pathogens, SCC and total bacterial count (TBC (85,000; 17,000), Staphylococcus aureus (587,000; 77,000); other streptococci (432,000; 108,000) and Streptococcus agalactiae (1,572,000; 333,000 RESUMO Este estudo foi realizado com objetivo de estabelecer a relação entre contagem de células somáticas (CCS) e a liberação de bactérias de quartos mamários de acordo com os patógenos da mastite. Amostras de leite de 638 quartos mamários foram examinadas para identificação dos patógenos da mastite, CCS e contagem total de bactérias (CTB). Estatísticas descritivas foram utilizadas para avaliar os dados brutos de CCS e CTB. A diferença entre médias para CCS e CTB de acordo com os resultados dos exames bacteriológicos foi avaliada pelo teste T para amostras independentes. Foram realizadas a correlação de

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“…Corynebacterium bovis is one of the most common bacteria isolated from milk samples that are submitted for the identification of intramammary infection (IMI)-causing pathogens around the world (Haltia et al 2006, Tenhagen et al 2006, Souza et al 2009). Despite its high prevalence, C. bovis is regarded as a minor pathogen with limited clinical significance (Huxley et al 2004) and has previously been regarded as a commensal organism (Brooks & Barnum 1984).…”

Section: Introductionmentioning

confidence: 99%

Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis

et al. 2015

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RESUMO.-[Expressão de CD14 e dos receptores do tipo toll 2 e 4 por neutrófilos lácteos provenientes de glândulas infectadas por Corynebacterium bovis.] O presente estudo objetivou avaliar alterações na expressão de CD14, e dos receptores do tipo toll (TLR) 2 e 4 na superfície de neutrófilos lácteos provenientes de glândulas mamárias infectadas por Corynebacterium bovis. O presente estudo utilizou 23 quartos negativos no exame bacteriológico, sem alterações na prova de fundo escuro e com contagem automática de células somáticas menor que 1 x10 5 células/mL, e 14 quartos mamários infectados por C. bovis A identificação de neutrófilos, assim como a porcentagem de neutrófilos lácteos que expressaram CD14, TLR2 e 4 foram avaliadas por citometria de fluxo utilizando anticorpos monoclonais. A porcentagem de neutrófilos que expressaram TLR2 e TLR4 nos quartos mamários infectados por C. bovis não diferiu dos quartos mamários sadios, assim como na expressão de TLR4. No entanto, a intensidade de fluorescência do TLR2 na superfície dos neutrófilos foi menor nos quartos mamários infectados por C. bovis. A porcentagem de neutrófilos que expressaram CD14 e a intensidade de fluorescência da molécula de CD14 foi menor na superfície dos neutrófilos lácteos dos quartos infectados por C. bovis. This study evaluated the expression of CD14, toll-like receptor (TLR) 2 and TLR4 on the surface of milk neutrophils in bovine mammary glands infected with Corynebacterium bovis. Here, we used 23 culture-negative control quarters with no abnormal secretion on the strip cup test and milk somatic cell count lower than 1x10 5 cells/mL, and 14 C. bovis infected quarters. The identification of neutrophils, as well as, the percentage of neutrophils that expressed CD14, TLR2 and TLR4 were analyzed by flow cytometry using monoclonal antibodies. The present study encountered no significant difference in the percentages of milk neutrophils that expressed TLR2 and TLR4 or in the expression of TLR4 by milk neutrophils. Conversely, a lower median fluorescence intensity of TLR2 in milk neutrophils was observed in C. bovis-infected quarters. The percentage of neutrophils that expressed CD14 and the median fluorescence intensity of CD14 in milk neutrophils was also lower in C. bovis-infected quarters.INDEX TERMS: Immune response, pattern recognition receptor, mastitis, milk, polymorphonuclear neutrophil leukocyte.

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Variação da contagem de células somáticas em vacas leiteiras de acordo com patógenos da mastite

Cited by 41 publications

References 9 publications

Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples

Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples

Relationship between total bacteria counts and somatic cell counts from mammary quarters infected by mastitis pathogens

Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis

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