Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras

Abstract

Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras RESUMOEm três experimentos, avaliou-se a sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras. No experimento 1, o sêmen foi diluído, 1:10, em 12 soluções (quatro diluidores x três crioprotetores -dimetilsulfóxido (DMSO), metilglicol ou glicerol). Metade de cada amostra foi resfriada por uma hora e a outra, criopreservada. A motilidade espermática foi avaliada imediatamente após a diluição e após o resfriamento em todas as amostras e, após o descongelamento, apenas nas amostras criopreservadas em DMSO. No experimento 2, o sêmen foi diluído, 1:5, em cinco soluções contendo DMSO e resfriado, criopreservado e avaliado como no experimento 1. No experimento 3, o sêmen foi diluído, 1:5, em quatro soluções contendo DMSO e criopreservado e avaliado quanto à motilidade e à fertilidade. Quando o sêmen foi diluído 1:10, observou-se motilidade acima de 58% em todas as amostras resfriadas em DMSO e em NaCl-tris-metilglicol. Baixa motilidade foi observada nas amostras resfriadas nas outras combinações com metilglicol (5-32%) ou glicerol (0-8%) e naquelas criopreservadas (16-20%). Todas as amostras diluídas 1:5 apresentaram motilidade de 65-72% após o resfriamento e de 45-66% após o descongelamento (experimentos 2 e 3). As taxas de eclosão produzidas com sêmen criopreservado, entretanto, foram baixas (17-23%) em relação ao sêmen fresco (60%).Palavras-chave: dourado, Salminus brasiliensis, diluidor, crioprotetor, criopreservação, fertilização ABSTRACTThe sensitivity of dourado (Salminus brasiliensis) spermatozoa to different cryoprotectant solutions was evaluated in three experiments. In experiment 1, semen was diluted, 1:10, in 12 solutions (four extenders x three cryoprotectants -dimethylsulphoxide (DMSO), methyglycol, or glycerol). Half of each sample was refrigerated for one hour while the other half was cryopreserved. Sperm motility was immediately assessed after dilution and after refrigeration in all samples, and after thawing in those cryopreserved in DMSO. In experiment 2, semen was diluted, 1:5, in five solutions containing DMSO, refrigerated, cryopreserved, and analyzed as in experiment 1. In experiment 3, semen was diluted, 1:5, in

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

“…The dilution ratio affects the sperm motility rate after cryoconservation (Viveiros et al, 2009). For lane snapper sperm were no significant differences between the proportions of 1:1 and 1:3 (P> 0.05), while for the other ratios evaluated motility rate and motility time decreased significantly (P <.05).…”

Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

“…In Brazilian fish species, some cryoprotectants have been tested and, in most of these studies, dimethyl sulphoxide (DMSO) at concentrations of 5-15% was the most effective (Table 5). More recently, the use of methylglycol as cryoprotectant has yielded higher post-thaw motility when compared to DMSO in sperm of all species tested (B. nattereri, B. orbignyanus, L. obtusidens and P. lineatus; Table 5), except for S. brasiliensis (Viveiros et al 2008a). Egg yolk and milk powder, known for their membrane-stabilizing activity, are Cruz-Casallas et al (2004 and Shimoda (2004) frequently added to the extender, typically in combination with glucose (Table 5).…”

<b>Induced reproduction of dourado (<i>Salminus brasiliensis</i>): fertilization with sperm cryopreserved in ACP^®-104

“…Evaluations of sperm quality of S. brasiliensis semen cryopreserved in standard solution showed that the highest values of motility were obtained in samples diluted in cryoprotectant solution at a ratio of 1:5, compared to samples diluted at 1:10 (Viveiros et al, 2009b). Carolsfeld et al (2003) recommend a dilution between 1:3 and 1:5 for the sperm of South American fish.…”

“…The use of cryopreserved dourado sperm has begun with Coser, Godinho and Ribeiro (1984), and resumed years later by Carolsfeld, Godinho, Zaniboni-Filho and Harvey (2003), Viveiros, Oliveira, Maria, Orfão, and Souza (2009b), Zanandrea, Weingartner and Zaniboni-Filho (2014) 2015). Most studies use sperm motility as a parameter for sperm quality, but, according to Bobe & Labbé (2010), only the success of fertilization allows to prove accurately the quality of sperm and oocyte.…”