Diversidade genética de estoques de reprodutores de Colossoma macropomum

Lopes, T. S. de S.; Júnior, Danilo Pedro Streit; Ribeiro, Ricardo Pereira; Povh, Jayme Aparecido; Lopera-Barrero, Nelson Maurício; Vargas, Lauro; Filho, Carlindo Pinto; Queiroz, J. R.

doi:10.1590/s0102-09352009000300029

Cited by 14 publications

(20 citation statements)

References 14 publications

(12 reference statements)

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“…Lopes et al (2009) analisaram dois estoques de C. macropomum, oriundos do Estado de Rondônia, e encontraram valores maiores Tabela 1. Sequência dos iniciadores e de nucleotídeos, porcentagem de bases púricas (G+C), e número e pares de bases dos fragmentos observados nos estoques de Colossoma macropomum.…”

Section: Resultsunclassified

“…Além disso, a variabilidade genética é fundamental para qualquer programa de melhoramento genético (Falconer, 1987). Portanto, a identificação do grau de variabilidade nos estoques de reprodutores é de grande importância para iniciar um programa de melhoramento (Lopes et al, 2009), pois o potencial evolutivo e de melhoramento depende da variabilidade genética (Melo et al, 2006). Assim, a correta identificação de estoques pode servir como ferramenta para o estabelecimento de bases de seleção, em programas de melhoramento.…”

Section: Introductionunclassified

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Variabilidade genética em tambaquis (Teleostei: Characidae) de diferentes regiões do Brasil

Jacometo

Barrero

Rodríguez-Rodríguez

et al. 2010

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a diversidade genética em quatro estoques de tambaquis (Colossoma macropomum) de diferentes regiões do Brasil, por meio de marcador RAPD. Foram utilizados 10 iniciadores para analisar 116 indivíduos, coletados de pisciculturas nos municípios de Urupá, RO, Teixeirópolis, RO, Neópolis, SE e Sorriso, MT. Foram encontradas diferenças nas frequências de 67 fragmentos, com um fragmento exclusivo em Sorriso e dois em Neópolis. Observaram-se altos valores de polimorfismo (72,92 a 83,33%), diversidade genética de Nei (0,27 a 0,30) e índice de Shannon (0,39 a 0,45). A análise da variância molecular, demonstrou que a maior parte da variação está dentro de cada estoque e não entre os estoques.A identidade e a distância genética entre os agrupamentos variou de 0,93 a 0,98 e 0,02 a 0,07, respectivamente, com menos distância entre os agrupamentos Urupá x Sorriso e entre Teixerópolis x Neópolis. A diferenciação genética variou de baixa a moderada (F st = 0,03 a 0,15) e o número de migrantes por geração foi alto (Nm = 5,96 a 24,3), entre os agrupamentos. Os estoques apresentam alta variabilidade e baixa diferenciação e distância genética entre si.Termos para indexação: Colossoma macropomum, diversidade genética, RAPD. Genetic variability of tambaqui (Teleostei: Characidae) from different regions of BrazilAbstract -The objective of this study was to evaluate the genetic diversity of four broodstocks of tambaqui (Colossoma macropomum) from different regions of Brazil using RAPD markers. Ten primers were used to analyze 116 individuals, collected from fish cultures of three municipalities in Brazil: Urupá, RO; Teixeirópolis, RO; Neópolis, SE; and Sorriso, MT. Differences in the frequencies of 67 fragments were found, with a unique fragment in Sorriso and two in Neópolis. High values of polymorphism (72.92 to 83.33%), Nei's genetic diversity (from 0.27 to 0.30) and Shannon index (from 0.39 to 0.45) were observed. Analysis of molecular variance showed that most of the variation is within each broodstock and not among them. The identity and genetic distance among the groups ranged from 0.93 to 0.98 and from 0.02 to 0.07, respectively, with less distance between clusters Urupá x Sorriso and Teixerópolis x Neópolis. Genetic differentiation ranged from low to moderate (F st = 0.03 to 0.15) and the number of migrants per generation was high (Nm = 5.96 to 24.3) among the groups. Stocks have high variability and low genetic differentiation and distance among them.

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Section: Resultsunclassified

Section: Introductionunclassified

Variabilidade genética em tambaquis (Teleostei: Characidae) de diferentes regiões do Brasil

Jacometo

Barrero

Rodríguez-Rodríguez

et al. 2010

Pesq. agropec. bras.

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“…Dominant marker in current assay proved efficient in the study of genetic variability. Other studies with the same marker for S. brasiliensis (ALMEIDA et al, 2013;GOMES et al, 2011GOMES et al, , 2013LOPES et al, 2007) and in other fish species (JACOMETO et al, 2010;, 2010LOPES et al, 2009;PAULA et al, 2006;POVH et al, 2008b) also gave positive results. In fact, dominant markers still have great possibilities of information for the initial review of genetic processes in natural populations or in fish stocks.…”

Section: Groupingmentioning

confidence: 81%

Genetic diversity of Salminus brasiliensis wild populations in downstream and upstream Cachoeira Branca, Verde River MS Brazil: a preliminary view

et al. 2016

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Although hydroelectric power plants generate most electricity in Brazil, the dams provide environmental barriers which also include the interruption of fish migration. Current study, conducted prior to the construction of São Domingos HPP on the shaft of the Cachoeira Branca (waterfall) on the Verde river, state of Mato Grosso do Sul, Brazil, evaluated the genetic diversity of two natural populations of dourado (Salminus brasiliensis) located downstream (Population A -PopA) and upstream (Population B -PopB) the waterfall. Eight primers were employed to analyze 56 specimens (PopA: 26; PopB: 30) and observed in 102 fragments, of which 86 were polymorphic (84.3%). Low frequency fragments (PopA: 2; PopB: 1), three limiting fragments (PopA) and three exclusive fragments (PopB) were identified. The intra-population genetic variability calculated the Shannon index, whilst the percentage of polymorphic fragments showed high rate variability within each population (PopA: 0.300 and 60.80% and PopB: 0.411 and 79.40%, respectively). The distance and genetic identity revealed high genetic differentiation (0.076 and 0.927, respectively). Results reveal that populations have high intra-population genetic variability and genetic differentiation between them, with low gene flow. ResumoAs usinas hidrelétricas são responsáveis por gerar a maioria da energia elétrica utilizada no Brasil, entretanto, suas construções podem gerar entraves ambientais, entre eles a interrupção da migração de peixes. O presente estudo foi realizado no período anterior a construção da Usina Hidroelétrica (UHE) São Domingos sobre o eixo da cachoeira Branca, no rio Verde -Mato Grosso do Sul (Brazil), e teve como objetivo avaliar a diversidade genética de duas populações naturais de dourado (Salminus brasiliensis) localizadas à jusante (População A -PopA) e montante (População B -PopB) da cachoeira. Foram utilizados oito iniciadores para analisar 56 indivíduos (PopA: 26; PopB: 30). Observaram-se um total de 102 fragmentos, dos quais 86 foram polimórficos (84,3%). Foram identificados fragmentos de baixa frequência (PopA: 2; PopB: 1), três fragmentos limitantes (PopA) e três fragmentos exclusivos (PopB). A variabilidade genética intra-populacional calculada com o índice de Shannon e pela porcentagem de fragmentos polimórficos mostrou altos valores de variabilidade dentro de cada população (PopA: 0,300 e 60,80% e PopB: 0,411 e 79,40%, respectivamente). A distância e identidade genética mostraram uma alta diferenciação genética (0,076 e 0,927, respectivamente). Conclui-se que as populações apresentaram alta variabilidade genética intra-populacional e diferenciação genética entre si, com baixo fluxo gênico.

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“…In previous studies using RAPD, the Shannon index for broodstocks in Rondônia state ranged from 0.44 to 0.46 (Lopes et al, 2009), and in four stocks from different regions of Brazil it ranged from 0.39 to 0.45 (Jacometo et al, 2010). According to Nei's (1973) measure of genetic diversity, average heterozygosity of the three broodstocks was 0.336, with the lowest estimate found for Balbina stock (0.283).…”

Section: Genetic Diversitymentioning

confidence: 88%

“…The first tambaqui broodstocks in Brazil were reared in the 1980s from a few wild fish; however, inbreeding reduced stock variability over the years, compromising fish vigor and lowering their resistance to management practices and diseases (Calcagnotto and Toledo-Filho, 2000). After a number of years, the genetic quality of new tambaqui broodstocks in the states of Mato Grosso and Rondônia was assessed using molecular markers (Lopes et al, 2009). A national genetic improvement program was then established, to monitor genetic variability and cross-mating within and between different stocks.…”

Section: Introductionmentioning

confidence: 99%

Impacts of stocking on the genetic diversity of Colossoma macropomum in central Amazon, Brazil

et al. 2016

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ABSTRACT. Tambaqui (Colossoma macropomum) is the main fish species farmed on a commercial scale in northern Brazil. In view of the current scenario of Brazilian aquaculture, studies on the genetic improvement and reproductive management of captive tambaqui are crucial in identifying the genetic variability of broodstocks and devising management practices. Genetic diversity of three tambaqui broodstocks in western Amazon was evaluated using molecular markers. Fin samples were collected from 89 fish; 38 from Balbina, 30 from a hatchery in Rio Preto da Eva, and 21 from the experimental farm of the Federal University of Amazonas (UFAM). Ten primers were used for the analysis of diversity and genetic structure. Of the 152 bands produced, 146 were polymorphic. The proportion of polymorphic loci showed little variation among the three stocks. The lowest and highest rates were found in the Rio Preto da Eva (80.92%) and Balbina (85.53%) stocks, respectively. Heterozygosity (H) and Shannon (I) indices were similar among the stocks; the lowest values were found in Balbina (H = 0.279 and I = 0.419), and the highest in UFAM (H = 0.294 and I = 0.439). Following analysis of the genetic structure and relationship, the sample was divided into two groups, with the Balbina stock clearly deviating from the others. The results suggest that, to increase genetic variability, molecular information may be used instead of replacement of wild breeders. The groups characterized here can be used in genetic improvement programs with other tambaqui broodstocks from different areas of South America.

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Diversidade genética de estoques de reprodutores de Colossoma macropomum

Cited by 14 publications

References 14 publications

Variabilidade genética em tambaquis (Teleostei: Characidae) de diferentes regiões do Brasil

Variabilidade genética em tambaquis (Teleostei: Characidae) de diferentes regiões do Brasil

Genetic diversity of Salminus brasiliensis wild populations in downstream and upstream Cachoeira Branca, Verde River MS Brazil: a preliminary view

Impacts of stocking on the genetic diversity of Colossoma macropomum in central Amazon, Brazil

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