2009
DOI: 10.1590/s0102-09352009000100021
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Abstract: Fifty-four samples were collected from growing and finishing pigs for the molecular diagnosis of enzootic porcine pneumonia. Nineteen lung fragments were obtained from pigs that showed signs of respiratory disease and 35 nasal swabs were obtained from clinically healthy pigs. For the detection of the bacterial genome in the samples, the nested PCR technique was used to amplify a fragment of 706bp. This fragment was subsequently cloned and sequenced. The sequence of obtained nucleotides was compared with six ot… Show more

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Cited by 3 publications
(2 citation statements)
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“…According to Vicente et al (2013), 52% of samples collected from different farms in a central region of São Paulo state were seropositive for M. hyopneumoniae. Silva et al (2009) also detected this agent in 52.6% of lung samples collected from southeast Brazil. Moreover, specific data on prevalence per country are not available in the literature, as the disease is not considered obligatory to report and does not limit commercial trade (Maes et al, 2018).…”
Section: Resultsmentioning
confidence: 84%
“…According to Vicente et al (2013), 52% of samples collected from different farms in a central region of São Paulo state were seropositive for M. hyopneumoniae. Silva et al (2009) also detected this agent in 52.6% of lung samples collected from southeast Brazil. Moreover, specific data on prevalence per country are not available in the literature, as the disease is not considered obligatory to report and does not limit commercial trade (Maes et al, 2018).…”
Section: Resultsmentioning
confidence: 84%
“…Mycoplasma hyopneumoniae is one of the most economically impacting respiratory pathogens in the world, detected in almost all swine farms with intensive production countries (Thacker 2006, Silva et al 2009, Maes et al 2018). The bacterium is considered a primary agent of swine enzootic pneumonia and respiratory complex, characterized by coughs, reduced growth rate and high feed conversion rate (Maes et al 1996).…”
Section: Introductionmentioning
confidence: 99%