Coloração de Ziehl-Neelsen como método rápido de diagnóstico de paratuberculose ovina

Coelho, Ana Cláudia; Pinto, Maria de Lurdes; Coelho, Andreia; Rodrigues, Jorge

doi:10.1590/s0102-09352008000500009

Cited by 4 publications

(5 citation statements)

References 16 publications

(10 reference statements)

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“…The detection of Map by PCR in blood supports the hypothesis that Map spread by the circulatory system as described by other authors (Bhide et al, 2006, Coelho et al, 2008b, Gwózdz et al, 2012. Our results showed that blood samples of some cases had sufficient bacterial DNA to identify Map by PCR, even when faecal analysis, culture from tissue or PCR from these samples were negative.…”

Section: Discussionsupporting

confidence: 86%

“…The PCR analysis procedure for colonies, faeces, tissues and blood was performed as described in the literature (Coelho et al, 2008b). Briefly, 1 g of faeces or tissue was homogenized in a Stomacher lab blender (Seward Stomacher 400) for 30 sec.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…Diagnosis based on the detection of IS900-specific sequences of Map by polymerase chain reaction (PCR) from tissue, faeces and blood is considered to be very quick, and highly specific (Bhide et al, 2006, Coelho et al, 2008b, Garrido et al, 2000. The aim of this study was to describe and compare the performance of four diagnostic methods (Zielh-Nielsen stainign, faecal culture, ELISA and PCR) for the detection of Map in tissues, faeces and blood in clinically suspected animals.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Mycobacterium avium subsp. paratuberculosis by several diagnostics techniques in clinical suspected sheep

Coelho

Coelho²,

García‐Díez

et al. 2018

J Hellenic Vet Med Soc

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A total of thirty sheep with clinical symptomatology of paratuberculosis (Johne’s disease) were subjected to four diagnostic techniques: histopathological examination, bacteriological culture (in faeces and tissues), polymerase chain reaction (PCR) (in blood, tissue and faecal samples) and antibody responses (ELISA). Twenty-one (70.0%) animals showed histological lesions. Bacterial culture of both faeces and tissue revealed that 2 (6.7%) and 6 (20.0%) of the 30 sheep were infected, respectively. Mycobacterium avium subsp. 24 paratuberculosis (Map) was identified in 4 animals via PCR of faeces (13.3%), and in 19 (63.3%) by PCR in tissues. PCR in blood revealed 7 (23.3%) infected animals. Three (10.0%) animals showed antibodies against Map. A greater number of positive animals were detected by histopathological examination and PCR in tissues than by culture or ELISA. This study confirmed the clinical findings and results suggest that histopathology, PCR in tissues and in blood can improve the detection of Map in physically suspected animals and should be considered useful tools in the diagnosis of Map in suspected sheep.

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Section: Discussionsupporting

confidence: 86%

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Mycobacterium avium subsp. paratuberculosis by several diagnostics techniques in clinical suspected sheep

Coelho

Coelho²,

García‐Díez

et al. 2018

J Hellenic Vet Med Soc

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“…The gross and microscopic lesions observed were consistent with those previously documented [5, 7, 21]. Our results agree with previous studies that suggest that smears stained with Ziehl-Neelsen are good indicators of infection and could be used to easily and rapidly screen tissue for the presence of compatible organisms that could be confirmed later by other methods with the added advantage of being inexpensive and fast [8]. …”

Section: Discussionsupporting

confidence: 92%

Detection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methods

Matos

Figueira

Martins

et al. 2017

The Journal of Veterinary Medical Science

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Paratuberculosis or Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The present work is part of a wider set of studies designed to assess the prevalence of paratuberculosis in free ranging red deer (Cervus elaphus). With that purpose, 877 free-ranging red deer legally hunted in the Centre-eastern Portugal were submitted to necropsy and sampled for molecular methods, microbiology and histopathology. Thirty-seven (4.2%) kidneys revealed acid-fast bacilli when screened with the Ziehl-Neelsen technique. Map was detected by IS900 polymerase chain reaction (PCR) in thirty (81.1%) of the Ziehl-Neelsen positive kidneys. Subsequent PCR and/or culture from the different organs of the 37 examined animals allowed us to detect 86.4% (32 animals) infected red deer. Our results suggest that renal involvement in Map infected deer may be underdiagnosed and thus the routine examination of this organ and its inclusion in PCR techniques designed for Map detection could substantially improve the diagnostic of paratuberculosis in red deer.

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“…Acid-fast staining technique of suspected tissues is rapid and requires little optimization and can be used as a screening method to detect bacilli either in feces or in tissues samples (Huntly et al, 2005;Coelho et al, 2008). In present study, ZN staining identified more positive samples among tissues samples from animals showing clinical sings and diffuse histopathologic lesions, similar findings were also observed by others (Hemalatha et al, 2013).…”

Section: Discussionsupporting

confidence: 88%

First Pathological, Molecular and Serological Investigation of Ovine Johne's Disease (Paratuberculosis) in Northeastern Algeria

Rezig¹,

Bouzid²,

Atia³

et al. 2021

BUASVMCN-VM

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Paratuberculosis (PTCB) or Johne's disease (JD) caused by a slow growing acid fast bacillus Mycobacterium avium subspecies paratuberculosis (MAP), is an infectious disease of many species including humans. The disease is responsible for important economic losses to livestock industry worldwide. Although the disease is widespread, it had not been studied in Algerian sheep. In this study we inspected the presence of the infection in sheep (aged 2 years old or older) using histopathology and IS900-PCR techniques in randomly 378 tissues (ileum, ileoceacale valve and lymph nodes) and fecal samples. Gross lesions were detected in 16 (4%) of samples. Histopathological examination revealed the presence of pathognomonic lesions of JD in 61 (14%) animals. Ziehl-Neelsen (ZN) staining of tissue samples was positive in 28 (7%) cases. MAP-DNA was detected in 34 (9%) fecal samples. 14 (4%) animals were tested positive for antibodies against MAP. This study showed that not only ovine paratuberculosis present in Algeria but infected animals are excreting the bacteria in feces. This could be a significant threat for other ruminants and humans. Other studies should be carried out in order to better understand the prevalence and the molecular epidemiology of MAP in the country.

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Coloração de Ziehl-Neelsen como método rápido de diagnóstico de paratuberculose ovina

Cited by 4 publications

References 16 publications

Detection of Mycobacterium avium subsp. paratuberculosis by several diagnostics techniques in clinical suspected sheep

Detection of Mycobacterium avium subsp. paratuberculosis by several diagnostics techniques in clinical suspected sheep

Detection of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> in kidney samples of red deer (<i>Cervus elaphus</i>) in Portugal: Evaluation of different methods

First Pathological, Molecular and Serological Investigation of Ovine Johne's Disease (Paratuberculosis) in Northeastern Algeria

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