Sulfato de condroitina e hialuronato de sódio no tratamento da doença articular degenerativa em cães: estudo histológico da cartilagem articular e membrana sinovial

Melo, Eliane Gonçalves de; Nunes, V.A.; Rezende, C.M.F.; Gomes, M.G.; Malm, Christina; Gheller, Valentim Arabicano

doi:10.1590/s0102-09352008000100013

Cited by 15 publications

(20 citation statements)

References 17 publications

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“…Although studies have confirmed that parenteral administration of chondroitin sulfate stimulates chondrocytes metabolism and satisfactorily reduces the progression of articular cartilage degeneration in DAD models induced by chronic instability in dog knee (Gonçalves et al, 2008;Melo et al, 2008), the results of the present study clarify that the chondroitin sulfate in association with glucosamine, as a nutraceutical, did not promote the same stimulus in the repair of sharply induced osteochondral defects.…”

Section: Discussioncontrasting

confidence: 69%

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Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Eleotério

Pontes²,

Machado³

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.Keywords: degenerative articular disease, osteoarthritis, arthrosis, chondroprotective, cartilage RESUMO Os nutracêuticos compostos de sulfato de condroitina e glucosamina são comumente utilizados no manejo da doença articular degenerativa na rotina veterinária. Entretanto, existem controvérsias sobre a contribuição dessas substâncias à cartilagem articular. O objetivo deste trabalho foi avaliar a eficácia de um nutracêutico veterinário à base de sulfato de condroitina e glucosamina na reparação de defeitos osteocondrais induzidos no côndilo femoral de cães, através de análises macroscópica, histológica e histomorfométrica. O nutracêutico foi administrado no dia seguinte à indução da lesão, pela via oral, a cada 24 horas (grupo tratado -GT, 24 animais), sendo comparado a animais que não receberam o produto (grupo controle -GC, de igual número de animais

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Section: Discussioncontrasting

confidence: 69%

“…However, the biochemical details of this therapeutic option are still not completely understood and further investigation is necessary (Blitterswijk et al, 2003), as the degenerative process has not been satisfactorily prevented or delayed yet (Melo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Eleotério

Pontes²,

Machado³

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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“…The model used CCLT to induce morphological changes allowed the evaluation of this common disease, as described by Biasi et al (2005), Gonçalves et al (2008) and Melo et al (2008). The development and progression of OA occurred due to joint instability, which changed the distribution of weight on the joint (Glasson et al 2007, Herzog & Longino 2007.…”

Section: Discussionmentioning

confidence: 99%

Experimental osteoarthritis in rabbits: lesion progression

et al. 2013

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ABSTRACT.-Campos W.N.S., Souza M.A., Ruiz T., Peres T.P., Néspoli P.B., Marques A.T.C., Colodel E.M. & Souza R.L. 2013. Experimental osteoarthritis in rabbits: lesion progression. Pesquisa Veterinária Brasileira 33(3):279-285. Programa de Pós-Graduação em Ciên-cias Veterinárias, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso, Av. Fernando Corrêa da Costa 2367, Bairro Boa Esperança, Cuiabá, MT 78068-900, Brazil. E-mail: wilmavet@gmail.comThe aim of this study was to evaluate the progression of lesions in different stages of osteoarthritis (OA) experimental by radiography (RX), computed tomography (CT), macroscopic and histopathology, linking these different diagnostic methods, helped to provide information that helps the best time for the therapeutic approach. Four experimental periods were delineated at 3, 6, 9 and 12 weeks after induction of OA, known as PI, PII, PIII and PIV, respectively, each with six animals. We evaluated the five compartments of the femorotibial joint: medial femoral condyle (MFC), lateral femoral condyle (LFC), medial tibial plateau (MTP), lateral tibial plateau (LTP) and femoral trochlea (FT). Therefore we established an index by compartment (IC) and by adding such an index was estimated joint femorotibial (IFT). It was observed that the CFM was the compartment with the highest IC also differed significantly (p<0.05) from other compartments. Compartments showed no significant difference (p>0.05) between the PI and PII, however contrary fact occurred between the PII and PIII (p<0.05), PIII and PIV (p<0.01) and between PI and PIV (p<0.001). Similarly the IFT, showed a significant difference in the animals of PIV compared to PI (p<0.001), PII (p<0.001) and PIII (p<0.01), and there was no statistical difference (p> 0.05) between the PI and PII. In the variation of the average interval between periods, there was a higher value between the PIII PIV and for the other intervals of time periods (PI, PII, and PIII-PII). However, these intervals showed no statistically significant difference (p>0.05). Through the RX, CT, macroscopic and histopathological findings, we found similar patterns among individuals within the same period demonstrating a gradual progression of the disease. These results show that between 3 and 6 weeks progression of the lesion is slower and probably also can be reversed in comparison to other ranges where proved further progression between 9 and 12 weeks after induction of trauma OA. These results may provide a better therapeutic approach aimed at reversing the lesions in early stages of OA. We conclude that the interconnection of the four diagnostic methods individually classified into scores, which were unified in both indices in the evaluation by the femorotibial joint compartment and may represent a diagnostic condition closer to the true condition of the injury and its progression. . Através do RX, TC, macroscopia e histopatológico, verificamos padrões similares entre os indivíduos dentro do mesmo período demonstrando assi...

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“…Melo et al 15 have stated that changes are expected in both groups due to joint instability. The imbalance in the distribution of forces on the articular surface led to the breakdown of proteoglycan arrangements, to the consequent increase in cartilage hydration (swelling) and to the exposure of collagen fibrils (fibrillation).…”

Section: Discussionmentioning

confidence: 97%

“…However, these changes were superficial and showed early osteoarthritis stages due to the collagenase concentration, according to Kikuchi et al 6 . The ACLT method has created degenerative cartilage lesions after 12 weeks, as well as more severe degradation 4,15,16 .…”

Section: Discussionmentioning

confidence: 99%

Comparison between two different experimental models of osteoarthritis in rabbits. Intra-articular collagenase injection and anterior cruciate ligament transection

Hermeto

Rossi²,

Jardim³

et al. 2016

Acta Cir. Bras.

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PURPOSE:To compare two different experimental models of osteoarthritis in rabbits: intra-articular collagenase injection and anterior cruciate ligament transection. METHODS:Ten adult rabbits were randomly divided in two groups: COLL (collagenase group) and ACLT (anterior cruciate ligament transection). The COLL group was treated with 0.5 ml collagenase solution (2mg collagenase/0.5 ml sterile PBS), and the ACTL group was subjected to anterior cruciate ligament. After six and twelve weeks, respectively, the animals in the COLL and ACTL groups were euthanized. The gross appearance and histological examinations conducted in the cartilage articular surface was blindly scored according to the criteria developed by Yoshimi et al. (1994) and Mankin et al. (1971), respectively. RESULTS:The gross morphologic observation, macroscopic score and histological examinations have demonstrated that the ACTL group presented the highest scores, and lesions more severe than those in the COLL group. CONCLUSIONS: Both methods, anterior cruciate ligament transection and collagenase, applied to the stifle joint of the rabbits have effectively induced degenerative changes in the cartilage tissue, through statistically significant analysis (p≤0.05). The ACTL method has presented more severe lesions.

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Sulfato de condroitina e hialuronato de sódio no tratamento da doença articular degenerativa em cães: estudo histológico da cartilagem articular e membrana sinovial

Cited by 15 publications

References 17 publications

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Experimental osteoarthritis in rabbits: lesion progression

Comparison between two different experimental models of osteoarthritis in rabbits. Intra-articular collagenase injection and anterior cruciate ligament transection

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