Double-staining immunohistochemistry of canine mammary neoplasms

Pereira, Fernando Lobo; Ferreira, Ema; Leitão, Dina; Cassali, Geovanni Dantas

doi:10.1590/s0102-09352006000400037

Arq. Bras. Med. Vet. Zootec.

2006

DOI: 10.1590/s0102-09352006000400037

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Double-staining immunohistochemistry of canine mammary neoplasms

Fernando Lobo Pereira¹,

Ema Ferreira²,

Dina Leitão

et al.

Abstract: Communication [Comunicação] Double-staining immunohistochemistry of canine mammary neoplasms [Dupla marcação imunoistoquímica em neoplasias mamárias caninas]

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Cited by 2 publications

(1 citation statement)

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“…The method allows to provide stable speci mens for light microscopy (Sinclai et al, 1986) but is only suitable for visualization of a single antigen per sec tion. Double staining with two enzyme reactions that produce brown and red products is sometimes used to localize two antigens (Pereira et al, 2006). However, the method is unacceptable if two antigens are colocalized in the same cell area: staining overlap does not allow to capture single colors (Van der Loos et al, 1993).…”

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confidence: 99%

Imaging of cell surface antigens on tumor lymph-node sections using fluorescence quantum dots

Raphalovskaya-Orlovskaya¹,

Горгидзе²,

Gladkih³

et al. 2012

Cell Tiss. Biol.

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In this work we explored the potential of quantum dots for fluorescent detection of lymphoid sur face antigens. To optimize detection with quantum dots, we upgraded a fluorescent microscope that allowed us obtaining multiple images from different quantum dots on a single section. Specimens stained with quan tum dots remained stable over two weeks and practically did not bleach under the mercury lamp during scores of minutes. Double staining of frozen sections with direct conjugates of quantum dots with primary mouse monoclonal antibodies demonstrated direct conjugate high specificity and sensitivity. High stability of quan tum dots' fluorescence allows their use in diagnostics to analyze antigen coexpression on lymphoid tissue sec tions. "Spillover" of fluorescent signals from quantum dots into adjacent fluorescent channels maximally sep arated by 40 nm did not exceed 8%, which renders spectral compensation unnecessary.

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mentioning

confidence: 99%

Imaging of cell surface antigens on tumor lymph-node sections using fluorescence quantum dots

Raphalovskaya-Orlovskaya¹,

Горгидзе²,

Gladkih³

et al. 2012

Cell Tiss. Biol.

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Linfoma Intranasal Em Cão: Aspectos Clínicopatológicos E Imunoistoquímicos

et al. 2016

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O linfoma canino raramente envolve a cavidade nasal. O diagnóstico pode ser estabelecido por meio da citologia, histopatologia e imunoistoquímica, onde a ultima é uma técnica valiosa para a identificação e classificação de células tumorais. O trabalho objetivou descrever um caso de linfoma intranasal em um canino, com o auxílio do imunodiagnóstico. Um canino, macho, sete anos de idade, sem raça definida, possuía aumento de volume em toda região nasal dorsal e lateral, em associação com uveíte anterior. Como exame complementar solicitou-se citologia de linfonodos e da tumoração nasal. A avaliação citológica dos gânglios linfáticos evidenciou a presença de formas amastigotas de Leishmania spp., enquanto a citologia da tumoração nasal exibiu padrão morfológico indicativo de linfoma. Optou-se pela eutanásia do animal e o mesmo foi encaminhado para necropsia. Os achados necroscópicos incluíram a presença de tumor em toda a extensão das cavidades nasais, ocasionando completa obliteração luminal e perda total dos ossos endoturbinados e conchas nasais. Foram coletados fragmentos do tumor nasal, fígado e globo ocular, os quais foram encaminhados para análise histopatológica, a qual revelou proliferação neoplásica, composta por células redondas, sendo sugerida a realização de análise imunoistoquímica para confirmação diagnóstica. O perfil imunoistoquímico aplicado detectou um linfoma de células Natural Killer. O linfoma intranasal em cães muitas vezes é negligenciado no diagnóstico diferencial das enfermidades do trato respiratório superior. Torna-se fundamental a realização do ensaio imunoistoquímico, pois equivale a uma valiosa ferramenta diagnóstica para os pacientes com linfoma.

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Double-staining immunohistochemistry of canine mammary neoplasms

Abstract: Communication [Comunicação] Double-staining immunohistochemistry of canine mammary neoplasms [Dupla marcação imunoistoquímica em neoplasias mamárias caninas]

Cited by 2 publications

References 5 publications

Imaging of cell surface antigens on tumor lymph-node sections using fluorescence quantum dots

Imaging of cell surface antigens on tumor lymph-node sections using fluorescence quantum dots

Linfoma Intranasal Em Cão: Aspectos Clínicopatológicos E Imunoistoquímicos

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