Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus

Alves, Camille Marques; Vasconcelos, A.C.; Martins, Almir de Sousa; Puerto, Helen L. Del; Santos, F.G.A.; Nunes, J.; Campos, Paula Peixoto; Moro, Luciana

doi:10.1590/s0102-09352006000400004

Cited by 5 publications

(7 citation statements)

References 21 publications

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“…The changes found in OD, SDtd and IOD values, in association with a reduction in area and perimeter, are typical of nuclei in chromatin fragmentation. The nuclear alterations described for the RTECs in present study are similar to those described for the thymus (24) and cerebellum (15) of dogs infected with distemper, suggesting that chromatin fragmentation due to cell death or low physico-chemical stability of DNA is an event closely associated with the tissue lesions that accompany the disease.…”

Section: Discussionsupporting

confidence: 83%

Renal Abnormalities Caused by Canine Distemper Virus Infection in Terminal Patients

Silva

Borin-Crivellenti

et al. 2022

Front. Vet. Sci.

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The aim of this study was to analyze the glomerular and tubular alterations in dogs with terminal distemper through light microscopy, immunofluorescence, and electron microscopy. Thirteen animals with a molecular diagnosis of distemper and neurological signs were selected. As a control group, 10 clinically healthy animals with no manifestations or signs of disease and with negative tests for Ehrlichia sp., Anaplasma sp., and Babesia sp. were included in this study. Renal tissue was evaluated by light microscopy, topochemistry for DNA/chromatin, and video image analysis to detect the nuclear phenotypes of the renal tubular epithelial cells (RTECs), immunofluorescence, and transmission electron microscopy. Results showed that dogs with distemper exhibited anemia, hypergammaglobulinemia, and proteinuria. Creatinine in the distemper group was lower compared to the control group (p = 0.0026), but there was no significant difference in relation to urea (p = 0.9876). Although this alteration may be due to the smaller muscle mass observed in animals with distemper, it probably is not of clinical importance. Glomerular and tubular lesions were confirmed by light microscopy in 84.6% of these animals. Additional findings in the animals with distemper included deposition of different classes of immunoglobulins, particularly IgM in 92.3% of the cases, fibrinogen deposition in 69.2% of the cases as assessed by immunofluorescence, alterations in the nuclear phenotypes of the RTEC characterized by condensation of chromatin, loss of DNA and reduction in the nuclear shape, and the presence of subendothelial and mesangial electron-dense deposits. These findings confirm the existence of renal alterations related to terminal distemper.

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Section: Discussionsupporting

confidence: 83%

Renal Abnormalities Caused by Canine Distemper Virus Infection in Terminal Patients

Silva

Borin-Crivellenti

et al. 2022

Front. Vet. Sci.

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“…Apoptosis might be triggered or inhibited by pathological (Batista et al, 2005;Alves et al, 2006;Batista et al, 2007) and physiological environmental stimuli (Arends and Wyllie, 1991).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Santos¹,

Vasconcelos²,

Nunes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in the regressive tumors. It was also confirmed by transmission electron microscopy, which showed cells with typical morphology of apoptosis and by the TUNEL reaction that detected in situ the 3'OH nick end labeling mainly in the regressive tumors. The regressive TVTs also showed an intensified immunostaining for P53 besides a more intense genomic DNA fragmentation detected by the agarose gel electrophoresis. In conclusion, apoptosis has an important role in the regression of the experimental TVT in a way that is P53-dependent.Keywords: dog, venereal tumor, apoptosis, cell death RESUMO Doze cães, adultos, machos e sem raça definida foram transplantados subcutaneamente, na região hipogástrica, com células originadas de dois tumores venéreos transmissíveis caninos (TVT). O objetivo do estudo foi demonstrar e quantificar a ocorrência de apoptose na regressão do TVT. Após seis meses

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“…Foram contados os pontos que incidiram sobre as células hematopoiéticas. No timo, foram utilizadas cinco lâminas/grupo/dia de nascido e mensurados 10 lóbulos tímicos para determinação da área tímica, área cortical e área medular (Alves et al, 2006). Todas as medidas foram efetuadas mediante o sistema analisador de imagens, Image Pro-plus, acoplado a um microscópio binocular da Olympus.…”

Section: Methodsunclassified

Ação da melatonina sobre a dinâmica sanguínea de ratas prenhes e sobre a histogênese do baço e do timo da prole

Araújo

Wanderley‐Teixeira

Vilaça-Junior

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus

Cited by 5 publications

References 21 publications

Renal Abnormalities Caused by Canine Distemper Virus Infection in Terminal Patients

Renal Abnormalities Caused by Canine Distemper Virus Infection in Terminal Patients

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Ação da melatonina sobre a dinâmica sanguínea de ratas prenhes e sobre a histogênese do baço e do timo da prole

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