Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes

Adona, Paulo Roberto; Leal, Cláudia Lima Verde

doi:10.1590/s0102-09352006000300011

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…2011), and after release of meiosis inhibition, it is possible that some mechanisms under control of these kinases are disturbed. Similar to accelerated meiosis resumption reported for such treatments (Adona and Leal 2006), degradation of certain transcripts could be also accelerated.…”

Section: Discussionsupporting

confidence: 71%

Gene Expression in Bovine Oocytes and Cumulus Cells After Meiotic Inhibition with the Cyclin‐Dependent Kinase Inhibitor Butyrolactone I

Leal

Mamo

Fair

et al. 2011

Reprod Domestic Animals

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The aim of this study was to determine the effect of temporary inhibition of meiosis using the cyclin-dependent kinase inhibitor butyrolactone I (BLI) on gene expression in bovine oocytes and cumulus cells. Immature bovine cumulus-oocyte complexes (COCs) were assigned to groups: (i) Control COCs collected immediately after recovery from the ovary or (ii) after in vitro maturation (IVM) for 24 h, (iii) Inhibited COCs collected 24 h after incubation with 100 μm BLI or (iv) after meiotic inhibition for 24 h followed by IVM for a further 22 h. For mRNA relative abundance analysis, pools of 10 denuded oocytes and respective cumulus cells were collected. Transcripts related to cell cycle regulation and oocyte competence were evaluated in oocytes and cumulus cells by quantitative real-time PCR (qPCR). Most of the examined transcripts were downregulated (p < 0.05) after IVM in control and inhibited oocytes (19 of 35). Nine transcripts remained stable (p > 0.05) after IVM in control oocytes; only INHBA did not show this pattern in inhibited oocytes. Seven genes were upregulated after IVM in control oocytes (p < 0.05), and only PLAT, RBP1 and INHBB were not upregulated in inhibited oocytes after IVM. In cumulus cells, six genes were upregulated (p < 0.05) after IVM and eight were downregulated (p < 0.05). Cells from inhibited oocytes showed the same pattern of expression regarding maturation profile, but were affected by the temporary meiosis inhibition of the oocyte when the same maturation stages were compared between inhibited and control groups. In conclusion, changes in transcript abundance in oocytes and cumulus cells during maturation in vitro were mostly mirrored after meiotic inhibition followed by maturation.

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Section: Discussionsupporting

confidence: 71%

Gene Expression in Bovine Oocytes and Cumulus Cells After Meiotic Inhibition with the Cyclin‐Dependent Kinase Inhibitor Butyrolactone I

Leal

Mamo

Fair

et al. 2011

Reprod Domestic Animals

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“…Previous studies have shown that meiosis resumption is accelerated as GVBD is observed earlier during maturation (Hashimoto et al. 2002; Adona and Leal 2006; Adona et al. 2008a).…”

Section: Discussionmentioning

confidence: 96%

“…Temporarily blocking meiosis in oocytes has been shown to accelerate the kinetics of the cell cycle in these cells irrespective of the duration of blocking culture or the kind of drugs used (Mayes and Sirard 2001; Adona and Leal 2006), causing a shortening of the duration of maturation with no effect on development. This phenomenon was well established by observations from our laboratory (Adona and Leal 2006; Adona et al. 2008a; b) and others (Ponderato et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cyclin‐dependent Kinase (CDK) Inhibition on Expression, Localization and Activity of Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase (MAPK) in Bovine Oocytes

Quetglas

Adona

et al. 2010

Reprod Domestic Animals

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This study aimed to assess the effects of cyclin-dependent kinase (CDK) inhibition on factors involved in the control of meiosis in bovine oocytes: maturation promoting factor (MPF) (p34(cdc2) and cyclin B1) and mitogen activated protein kinase (MAPK). Oocytes were maintained at germinal vesicle (GV) stage in vitro with 10 μM of the CDK inhibitor butyrolactone I (BLI) for 24 h (inhibited). After this period, some of the oocytes were transferred to in vitro maturation (IVM) culture for 24 h (inhibited and matured). Control oocytes were assessed immediately after follicle aspiration (immature) or after in vitro maturation for 24 h (matured). Real-time PCR analyses showed that transcripts for p34(cdc2) and MAPK were detected in immature and inhibited oocytes and decreased after maturation, irrespective of CDK inhibition with BLI. Cyclin B1 was detected at similar levels in all oocyte groups. The p34(cdc2) and MAPK proteins were detected by Western blotting at similar levels in all oocyte groups, and cyclin B1 protein was detected only after maturation. Immunofluorescence detection showed that p34(cdc2) was localized in the cytoplasm and GV of immature oocytes, and then throughout the cytoplasm after maturation. Cyclin B1 and MAPK were detected in the cytoplasm in all oocyte groups. Maturation promoting factor and MAPK activities were similar throughout most of maturation for oocytes treated with or without BLI. In conclusion, CDK inhibition did not affect the expression (mRNA and protein levels) and localization of MPF and MAPK, and had nearly no effect on kinase activities during maturation.

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“…SD, standard deviation. inhibitory substances has been tested, however no advances have been achieved in embryo production (Adona & Leal, 2006;Gharibi et al, 2013;Rose et al, 2013;Ulloa et al, 2014). Unlike other inhibitors, cilostamide, which inhibits PDE-3A, specifically acts in the oocyte to inhibit cAMP degradation and therefore retains meiotic progression (Sasseville et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes

Guimarães

Pereira

Kussano

et al. 2015

Zygote

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This study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 μM) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P 0.05) to the control. The deleterious effect of 20 μM cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 μM) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the best concentration and the arresting period to increase oocyte competence and embryo development.

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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes

Cited by 9 publications

References 15 publications

Gene Expression in Bovine Oocytes and Cumulus Cells After Meiotic Inhibition with the Cyclin‐Dependent Kinase Inhibitor Butyrolactone I

Gene Expression in Bovine Oocytes and Cumulus Cells After Meiotic Inhibition with the Cyclin‐Dependent Kinase Inhibitor Butyrolactone I

Effect of Cyclin‐dependent Kinase (CDK) Inhibition on Expression, Localization and Activity of Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase (MAPK) in Bovine Oocytes

The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes

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