Production and purification of beta-toxin from Clostridium perfringens type C

Carvalho, Augusto Vinicius Arruda de; Heneine, Luíz Guilherme Dias; Assis, Ronnie Antunes de; Abreu, V.L.V.; Gonçalves, Luciana Aramuni; Lobato, Francisco Carlos Faria

doi:10.1590/s0102-09352006000200018

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2008

2024

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“…Samples of C. perfringens type D, donated by the INTA -Argentina, were used for the production and purification of the epsilon prototoxin as described in Parreiras et al (2002) and Carvalho et al (2006).…”

Section: Methodsmentioning

confidence: 99%

Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine

Rocha¹,

Assis²,

Lobato³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, -20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.

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Section: Methodsmentioning

confidence: 99%