Association between IGF-IR gene polymorphisms and productive and reproductive traits in Holstein cows

Schoenau, William; Porciúncula, Patrı́cia Marafon; Zamberlan, G.; Mesquita, Fernando Silveira; Vieira, Viviane Figueiredo; Oliveira, João Francisco Coelho de; Gonçalves, P. B. D.

doi:10.1590/s0102-09352005000600011

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…The genomic DNA was extracted from the leucocytes using Gene JET genomic DNA extraction kits following the manufacturer protocol (Thermo Scientific). The IGF1 locus was amplified by using PCR forward primer5'-ATTACAAAGCT-GCCTGCCCC-3' and reverse primer CACATCT-GCTAATACACCTTACCCG-3' (Ge et al, 1997) while IGF1-R using forward primer 5'-ACCCGCC-AAGAAATTGTTTC-3'and reverse primer 5'-GGC-TCCTCCATACTTCCTG-TA-3' (Schoenau et al, 2005). The PCR reactions were performed with a total volume 25µl containing 1.0µl of forward and reverse primers (10 pmol), 3.0µl DNA template, 12.5µlMaster mix and 7.5µl nuclease free water.…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Polymorphism of IGF-1 and Its Receptor (IGF-1R) Genes and Their Association with Fertility Problems of Egyptian Buffaloes

Ramadan¹,

Abo-Gamil²,

El-Qaliouby³

2018

AJVS

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The objectives of our study were to detect genetic polymorphisms in insulin-like growth factor-1 (IGF-1) and insulin-like growth factor1-receptor (IGF-1R) genes by using SCCP and direct sequence methods and to investigate their possible associations with fertility problems in Egyptian buffaloes. A total number of 100 buffalo heifers at Mahallet Mousa farm in Kafr El-sheikh province, Egypt were investigated. Animals were assigned into two main groups: normal fertile (50 animals) and infertile due to anestrous (50 animals). Blood samples were collected and the genomic DNA was extracted then PCR were performed with annealing temperatures at 58 o C and 55 o C for IGF1 and IGF1-R respectively. SSCP was performed for all amplified samples and loaded into a non-denaturing 12% polyacrylamide gel. Four amplicon from both of IGF-1 and IGF-1R genes for the two investigated groups were sequenced using the same primers. 265-bp from 5untranslated region (5-UTR) of IGF-1 gene and 311-bp from IGF-1R (part of intron 12 and part of exon 13) were amplified. In all investigated samples there was one SSCP pattern for IGF-1 and for IGF-1R genes in anestrus and normal fertile buffaloes' groups Moreover there were no polymorphisms neither in IGF-1 nor IGF-1R after the sequencing of four samples from each buffaloes' group for both of IGF-1 and IGF-1R genes. It seems that IGF-1 and IGF-1R are highly conserved in the investigated buffalo's population.

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Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Polymorphism of IGF-1 and Its Receptor (IGF-1R) Genes and Their Association with Fertility Problems of Egyptian Buffaloes

Ramadan¹,

Abo-Gamil²,

El-Qaliouby³

2018

AJVS

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Effect of IGF1, GH, and PIT1 markers on the genetic parameters of growth and reproduction traits in Canchim cattle

et al. 2014

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The availability of dense genomic information has increased genome-wide association studies for the bovine species; however research to assess the effect of single genes on production traits is still important to elucidate the genes functions. On this study the association of IGF1, GH, and PIT1 markers with growth and reproductive traits (birth weight, weaning weight, weight at 12 and 18 months of age, preweaning average daily weight gain, age and weight at first calving, and scrotal circumference at 12 and 18 months of age) were assessed by means of the variance component approach. The phenotypes were adjusted and then analyzed under two animal models, one which considered the polygenic and genotype (IGF1, GH or PIT1 markers) effects (Model 1), and the other which considers only the polygenic effect (Model 2). When the likelihood ratio test and the Bonferroni correction was applied at 5 % significance level, the genetic markers for the IGF1, GH, and PIT1 genes did not influence significantly the traits (p > 0.002). However, evidence of association of IGF1 with birth weight (p = 0.06) and GH with weight at first calving (p = 0.03) and with weight at 12 months of age (p = 0.08) was observed. In conclusion we could not confirm the associations between IGF1, GH, and PIT1 and growth traits that were previously reported in Canchim cattle, and no association was observed between these genes and reproductive traits. Future studies involving functional markers of IGF1, GH and PIT1 genes may help to clarify the role of these genes in growth and reproductive processes.

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Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Oliveira¹,

Wallau²,

Loreto³

2009

Electron. J. Biotechnol.

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High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first protocol utilizes the enzyme chymosin, normally used as "rennet" in cheese production and which is easily obtained on the commercial market. The second protocol uses "rennet DNA extraction protocol" combined with the DNA binding capacity of glass powder (glass milk), which can easily be "home made". The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples.

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Association between IGF-IR gene polymorphisms and productive and reproductive traits in Holstein cows

Cited by 3 publications

References 19 publications

Polymorphism of IGF-1 and Its Receptor (IGF-1R) Genes and Their Association with Fertility Problems of Egyptian Buffaloes

Polymorphism of IGF-1 and Its Receptor (IGF-1R) Genes and Their Association with Fertility Problems of Egyptian Buffaloes

Effect of IGF1, GH, and PIT1 markers on the genetic parameters of growth and reproduction traits in Canchim cattle

Isolation of high quality DNA: a protocol combining “rennet” and glass milk

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