Efeito do resfriamento do sêmen eqüino sobre sua congelabilidade

Fürst, R.; Carvalho, G. R.; Fürst, M.C.O.; Ruas, José Reinaldo Mendes; Borges, Adriana; Mafilli, V.

doi:10.1590/s0102-09352005000500005

Cited by 14 publications

(7 citation statements)

References 14 publications

(4 reference statements)

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“…Abnormalities were classified as major or minor defects according to the Brazilian College of Animal Reproduction recommendations (Henry and Neves 1998). Additionally, a sample from each ejaculate was evaluated for sperm membrane integrity, using the HOST as described by Furst et al (2005). The semen was extended (1:100) in a hypo-osmotic solution (100 mOsm/ml) of sucrose, incubated at 37°C for 1 h and then fixed with buffered formaldehyde saline.…”

Section: Methodsmentioning

confidence: 99%

“…A further challenge is the availability of a suitable simple method to evaluate semen under field conditions. The hypoosmotic swelling test (HOST) has been suggested to be an accurate method, which is easily performed and does not require sophisticated equipment (Vidament et al 1998;Neild et al 2000;Furst et al 2005). Neild et al (2000) reported that horse stallions with <40% of sperm reacting positively to the HOST (a positive result indicating an intact plasma membrane) were sub-fertile.…”

Section: Introductionmentioning

confidence: 99%

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Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares

Canisso

Carvalho

Morel

et al. 2011

Equine Veterinary Journal

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares

Canisso

Carvalho

Morel

et al. 2011

Equine Veterinary Journal

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“…Assim como no sêmen fresco, a totalidade de patologias espermáticas no sêmen descongelado em cada tratamento foi baixa, próxima à encontrada por outros autores (23,24) .…”

Section: Resultsunclassified

Características Do Sêmen Caprino Descongelado Após a Adição De Ringer Lactato, Citrato De Sódio E Solução Tris

Dias¹,

Santos²,

Penitente-Filho³

et al. 2015

Ciênc. anim. bras.

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ResumoA criopreservação do sêmen é de grande importância para diversas biotecnologias da reprodução, como Inseminação Artificial (IA), Produção de Embriões In Vitro (PIV) e Injeção Intracitoplasmática de Espermatozoides (ICSI). Avaliou-se a estabilidade e persistência da motilidade e vigor dos espermatozoides, assim como alterações da membrana plasmática, após a adição de Ringer com Lactato, citrato de sódio 2,92% ou solução TRIS ao sêmen caprino descongelado. O sêmen foi coletado de dois bodes da raça Parda Alpina, realizando-se os procedimentos padrões de análise e criopreservação seminal. Após a descongelação do sêmen, foram adicionados os diluentes Ringer Lactato, citrato de sódio 2,92% ou solução TRIS, realizandose os Testes de Termorresistência (TTR), Supravital e Morfológico. No TTR, somente o grupo a que foi adicionada a Solução TRIS obteve motilidade e vigor por maior período (90 minutos; P< 0,05). Não foi encontrada influência da adição das soluções na análise morfológica, assim como no teste Supravital, o qual apresentou valores próximos aos encontrados para motilidade (P> 0,05). Concluiu-se que a adição das soluções não permite uma grande persistência da motilidade e vigor dos espermatozoides descongelados, porém a solução TRIS poderia ser utilizada para expansão de doses seminais utilizadas em biotecnologias reprodutivas in vitro. Palavras-chave: bode; diluente; espermatozoide; sêmen. AbstractCryopreservation of semen is

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“…In horses, it has been reported (Cochran et al ., ) that a fast thawing (75 °C for 7 s) has more beneficial effects on spermatozoa than a slow thawing (37 °C for 30 s). However, in some studies (Borg et al ., ; Vidament et al ., ; Fürst et al ., ), the semen viability did not differ between fast (75 °C 5–10 s −1 ) and slow (37 °C 30 s −1 ) thawing protocols. In this regard, lowering the thawing temperature and prolonging the thawing time might facilitate the practical handling of thawed equine semen under the field conditions.…”

Section: Introductionmentioning

confidence: 81%

“…Diluted semen was placed into 0.5‐ml straws, cooled to 4–5 °C for 60 min, as proposed by Fürst et al . (). Each ejaculate was equally distributed and frozen by the conventional technique (conventional group) or by a fast‐freezing rate in a cryogenic bath (fast‐freezing group).…”

Section: Methodsmentioning

confidence: 97%

Impact of using a fast-freezing technique and different thawing protocols on viability and fertility of frozen equine spermatozoa

2013

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The effects of freezing technique and thawing protocol on thawed semen viability and fertility were studied. Ejaculates from 5 stallions (n = 25) were frozen by conventional or a fast-freezing technique. Frozen semen was thawed by two thawing protocols (37 °C 30 s(-1) or 75 °C 7 s(-1) ). Thawed semen was evaluated by progressive motility, vigour, morphology and plasma membrane integrity. Mares (n = 25) were inseminated with 300 (n = 11) or 150 (n = 14) million spermatozoa. A greater (P < 0.05) vigour and progressively motile spermatozoa were detected, respectively, at thawing and after 20 min post-thawing in the fast-freezing technique than in the conventional one. Plasma membrane integrity was also greater (P < 0.05) in semen frozen with the fast-freezing technique. Semen viability was not affected by thawing protocol. Pregnancy rate using the fast-freezing technique was 76% (19/25), and did not differ (P > 0.05) between insemination doses. We concluded that the 150 million progressively motile spermatozoa per dose using a deep-horn insemination maximises the use of equine semen. The fast-freezing technique, as compared to the conventional one, efficiently preserves the viability and fertilising capacity of spermatozoa, indicating a new method to improve the fertility of frozen equine semen.

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Efeito do resfriamento do sêmen eqüino sobre sua congelabilidade

Abstract: RESUMO

Cited by 14 publications

References 14 publications

Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares

Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares

Características Do Sêmen Caprino Descongelado Após a Adição De Ringer Lactato, Citrato De Sódio E Solução Tris

Impact of using a fast-freezing technique and different thawing protocols on viability and fertility of frozen equine spermatozoa

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