Expressão gênica de caspases 3 e 8 em timo e baço de ratas recém-desmamadas e imunossuprimidas por glicocorticóide

Batista, J.J.; Martins, Alberto Mesaque; Moro, Luciana; Vasconcelos, A.C.

doi:10.1590/s0102-09352005000400006

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Apoptosis might be triggered or inhibited by pathological (Batista et al, 2005;Alves et al, 2006;Batista et al, 2007) and physiological environmental stimuli (Arends and Wyllie, 1991).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Santos¹,

Vasconcelos²,

Nunes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in the regressive tumors. It was also confirmed by transmission electron microscopy, which showed cells with typical morphology of apoptosis and by the TUNEL reaction that detected in situ the 3'OH nick end labeling mainly in the regressive tumors. The regressive TVTs also showed an intensified immunostaining for P53 besides a more intense genomic DNA fragmentation detected by the agarose gel electrophoresis. In conclusion, apoptosis has an important role in the regression of the experimental TVT in a way that is P53-dependent.Keywords: dog, venereal tumor, apoptosis, cell death RESUMO Doze cães, adultos, machos e sem raça definida foram transplantados subcutaneamente, na região hipogástrica, com células originadas de dois tumores venéreos transmissíveis caninos (TVT). O objetivo do estudo foi demonstrar e quantificar a ocorrência de apoptose na regressão do TVT. Após seis meses

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“…Apoptosis might be triggered or inhibited by pathological (Batista et al, 2005;Alves et al, 2006;Batista et al, 2007) and physiological environmental stimuli (Arends and Wyllie, 1991).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Santos¹,

Vasconcelos²,

Nunes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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“…The last cleavage occurs when an activated nuclease cleaves the DNA at linker sites between nucleosomes, resulting in fragments that are multimers of 180-200 bp [41]. The presence of LMW fragments is a typical picture of apoptosis in various cell systems [42][43][44][45][46][47][48]. It is important to mention that the DNA damage caused by PDT is related to the treatment dose, length, etc., and that the resulting DNA fragmentation does not necessarily lead to cell death.…”

Section: Discussionmentioning

confidence: 99%

DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

Margraf-Ferreira

Carvalho

Machado

et al. 2017

Photodiagnosis and Photodynamic Therapy

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Photodynamic therapy (PDT) is a modality of therapy that involves the activation of photosensitive substances and the generation of cytotoxic oxygen species and free radicals to promote the selective destruction of target tissues. This study analyzed the application of PDT to Tritrichomonas foetus, a scourged and etiological agent of bovine trichomoniasis, a sexually transmitted infectious disease. As it is an amitochondrial and aerotolerant protozoan, it produces energy under low O tension via hydrogenosome. T. foetus from an axenic culture was incubated with photosensitizer tetrasulfonated aluminium phthalocyanine and then irradiated with a laser source (InGaAIP) at a density of 4.5Jcm. The DNA integrity of the control and treated group parasites was analyzed by conventional gel electrophoresis and comet assay techniques. In previous results, morphological changes characterized by apoptotic cell death were observed after T. foetus was submitted to PDT treatment. In the treated groups, T. foetus DNA showed a higher concentration of small fragments, about 200pb, in gel electrophoresis after PDT. In the comet assay, the DNA tail percentage was significantly higher in the treated groups. These results demonstrate that PDT leads to DNA fragmentation with changes in nuclear morphology and apoptotic features.

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Vincristine modulates the expression of Ki67 and apoptosis in naturally occurring canine transmissible venereal tumor (TVT)

Özalp

Zık

Baştan

et al. 2012

Biotechnic & Histochemistry

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We investigated eight adult dogs that were brought to veterinary clinics with a history of transmissible venereal tumors (TVT). Our goal was to demonstrate the occurrence of apoptosis and the cessation of cell proliferation at every phase of scheduled chemotherapy for naturally occurring TVT. Tissue samples were collected immediately after weekly treatments with vincristine sulfate and processed for histological purposes. Sections 5 μm thick were stained by the TUNEL reaction for apoptosis and immunostained for Ki67 as a proliferation marker. We observed that after vincristine applications, tumor cell proliferation ceased and apoptosis increased. Ki67 HSCORE values were significantly lowered after the first and second treatments with the chemotherapeutic agent compared to controls, whereas TUNEL HSCORE values were significantly higher after two applications of vincristine compared to controls. Our results suggest that scheduled vincristine sulfate applications stabilize the induction of tumor regression by inducing apoptosis and preventing cell proliferation.

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Expressão gênica de caspases 3 e 8 em timo e baço de ratas recém-desmamadas e imunossuprimidas por glicocorticóide

Cited by 3 publications

References 19 publications

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

Vincristine modulates the expression of Ki67 and apoptosis in naturally occurring canine transmissible venereal tumor (TVT)

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