Efeito da concentração de ácido 3-indol-acético na ativação e crescimento in vitro de folículos pré-antrais ovinos

Andrade, Evelyn Rabelo; Seneda, Marcelo Marcondes; Alfieri, Amauri Alcindo; Oliveira, José Américo de; Figueiredo, José Ricardo de; Toniolli, Ricardo

doi:10.1590/s0102-09352005000300010

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…They are in accordance with many studies in which preantral follicles were cultured in the presence of relaxin in humans (Shirota et al, 2005), epidermal growth factor (EGF) and 3-indol-acetic acid (IAA) in ovine (Andrade et al, 2005), kit-ligand (KL) and fibroblast growth factor (FGF) in mouse (Nilsson and Skinner, 2004), and coconut water in caprine (Silva et al, 2004;Martins et al, 2005). In regard to follicular growth, in both culture period, all treatments were similar when compared to control and among them, except follicles cultured with 15µM of α-tocopherol, which showed a reduction of follicular diameter after one day of culture.…”

Section: Discussionsupporting

confidence: 87%

“…Otala et al (2002) reported, in human ovarian tissue cultured for 8, 24, and 48h with antioxidant Nacetilcistein, the same changes verified in this study. Similar findings were also observed after in vitro culture of preantral follicles in caprine (Silva et al, 2004), murine (Nilsson and Skinner, 2004), and ovine (Andrade et al, 2005) or after criopreservation in caprine (Celestino et al, 2007).…”

Section: Discussionsupporting

confidence: 63%

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Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Lima-Verde

Matos²,

Bruno³

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured ABSTRACTThe effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.Keywords: caprine, preantral follicle, α-tocopherol, ternatin RESUMO

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 63%

Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Lima-Verde

Matos²,

Bruno³

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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“…The duration of culture was based on the time course of primordial follicle activation in sheep [ 13 ]. The treatments tested were based on the best results of culturing sheep preantral follicles in our laboratory [ 14 ] whereas concentrations of FSH and EGF were based on previous reports of culturing pig granulosa cells recovered from primary and secondary follicles [ 19 ] and bovine small preantral follicles [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…The auxin, indol acetic acid (IAA) is found in blood and in several tissues, including lung, kidney, liver, and brain [ 12 ]. Previous studies from our team have demonstrated that sheep primordial follicles are successfully activated to grow in vitro after culturing in medium supplemented with 40 ng/mL IAA [ 13 ]. Moreover, the addition of IAA and EGF or EGF and FSH to the culture media were the most effective treatments to sustain the health and viability of growing sheep primordial follicles during in vitro culture [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

Andrade

Maddox-Hyttel

Landim-Alvarenga

et al. 2011

Veterinary Medicine International

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The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

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Cryopreservation of Spix's yellow-toothed cavy epididymal sperm using Tris- and coconut water-based extenders supplemented with egg yolk or Aloe vera

et al. 2021

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Efeito da concentração de ácido 3-indol-acético na ativação e crescimento in vitro de folículos pré-antrais ovinos

Abstract: Efeito da concentração de ácido 3-indol-acético na ativação e crescimento in vitro de folículos pré-antrais ovinos

Cited by 4 publications

References 22 publications

Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

Cryopreservation of Spix's yellow-toothed cavy epididymal sperm using Tris- and coconut water-based extenders supplemented with egg yolk or Aloe vera

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