Mastocitoma cutâneo canino: estudo de 45 casos

Rech, Raquel R.; Graça, Dominguita Lühers; Kommers, Gláucia D.; Sallis, Eliza Simone Viégas; Raffi, Margarida Buss; Garmatz, Shana Letícia

doi:10.1590/s0102-09352004000400004

Cited by 8 publications

(10 citation statements)

References 16 publications

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“…This histochemical method quantitatively evaluates the rate at which tumor cells are proliferating (growth rate) (Derenzini 2000). The AgNOR values found in this study corroborate the specific scientific literature, which reports average AgNOR values for grade II MCTs from 1.28 to 3.96 (Bostock et al 1989, Rech et al 2004. The larger the number of AgNORs observed in the nucleus of the neoplastic cells, the greater the proliferative activity of the tumor (Derenzini 2000).…”

Section: Discussionsupporting

confidence: 85%

Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

et al. 2019

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Due to the high prevalence of mast cell tumors (MCTs) in the diagnostic routine, several factors, especially prognostic, have been sought to determine the biological behavior of these neoplasms. Immunohistochemistry (IHC) is one of the main tools utilized to biologically differentiate more aggressive tumors from less aggressive ones. However, some immunostainings are influenced by formalin fixation, interfering with the results. This is both a retrospective and prospective study of MCTs diagnosed in laboratory routine. A total of 25 samples, without knowledge about fixation time, were analyzed in the retrospective study, whereas 12 samples, with known fixation times, were assessed in the prospective study. Two histologic grading systems (Patnaik and Kiupel), special staining of toluidine blue, and IHC for KIT and Ki67 proteins were applied in both studies. Additionally, two amplification systems (biotinylated and non-biotinylated) for Ki67 protein and counting of the argyrophilic nucleolar organizing regions (AgNOR method) were tested in the prospective study. In the retrospective study, greater agreement between the evaluating pathologists was observed when the Kiupel system was used. IHC staining for KIT protein was effective in both studies, regardless of fixation time. IHC staining for Ki67 protein was highly sensitive to formaldehyde, and staining failure was observed in 56% of the cases in the retrospective study. In the prospective study, samples fixed for longer than 24 hours showed a reduction in the number of stained cells (altering the determination of the cell growth fraction) or showed absence of IHC staining in both amplification systems. The use of the AgNOR method to evaluate the rate of cell proliferation may be an alternative when the fixation time of the neoplasm is unknown or longer than 24 hours.

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Section: Discussionsupporting

confidence: 85%

Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

et al. 2019

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“…Seções de 5 m foram submetidas à coloração de AgNOR segundo Rech et al (2004). Para definir o número de AgNOR, foram contados os pontos pretos no interior dos núcleos em cem células tumorais em campos aleatórios e representativos de maior aumento (1000x) e divididos por cem (Rech, 2003).…”

Section: Methodsunclassified

“…O número dessas regiões em cada núcleo está diretamente relacionado à proliferação celular e, portanto, pode servir como um indicador de potencial maligno de um tumor (Rech et al, 2004;Oliveira et al, 2005;Silva et al, 2006). Neste estudo, tanto os neurofibromas benignos e malignos quanto os schwannomas benignos e malignos tinham um alto número de AgNOR, presumivelmente devido à sua alta taxa de multiplicação celular.…”

Section: Não Foram Observadosunclassified

Aspectos histoquímicos e imunoistoquímicos nos neoplasmas do sistema nervoso periférico

Viott¹,

Ramos²,

Inkelmann³

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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Doze casos de tumores do sistema nervoso periférico, sete schwannomas e cinco neurofibromas foram diagnosticados entre 1964 e 2004, em caninos e bovinos. Histologicamente, as células predominantes nos schwannomas eram fusiformes organizavam-se em paliçada ou em feixes aleatórios. Nos neurofibromas as células neoplásicas eram fusiformes e se originavam da periferia dos nervos formando ninhos e feixes. O tecido conjuntivo era mais abundante nos neurofibromas e os colágenos dos tipos I e III eram os principais constituintes desses neoplasmas. Os neurofibromas foram caracterizados por uma concentração alta e difusa de mastócitos, provavelmente devido à origem das células neoplásicas do perineuro e epineuro. A coloração de AgNOR não se mostrou eficiente como indicador de prognóstico nos neoplasmas analisados. Imunoistoquímicamente houve forte marcação para vimentina (100%) e S100 (100%) em ambos os tipos de tumor. A maioria dos schwannomas (75%) foi positiva para a proteína ácida fibrilar glial; os neurofibromas só apresentaram marcação nas células de Schwann dos fascículos nervosos.

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“…É geralmente encontrada na pele e subcutâneo, podendo acometer também medula óssea e outros órgãos (LONDON & SEGUIN, 2003). De todas as neoplasias cutâneas encontradas em cães, o mastocitoma está entre as mais comuns, representando cerca de 10 a 15% (Conroy, 1983;Pulley e Stannard, 1990;Goldschimidt e Shofer, 1992) e ainda, 11 a 27% de todos os tumores malignos reportados nesta espécie (Thamm e Vail, 2001, 2006.…”

Section: Introductionunclassified