Atividade secretória de tubas uterinas com e sem patologia de porcas adultas nas fases folicular e luteínica

Abstract

RESUMODescrevem-se as características anatomopatológicas e histoquímicas de 124 tubas uterinas de porcas (matrizes) abatidas em matadouros, nas fases folicular e luteínica do ciclo estral. As tubas uterinas foram distribuídas em quatro grupos: LSP-fase luteínica sem patologia tubárica (n=45), LCP-fase luteínica com patologia tubárica (n=19), FSP-fase folicular sem patologia tubárica (n=45) e FCP-fase folicular com patologia tubárica (n=15). Para estudo histoquímico de mucossubstâncias foram utilizadas as colorações de periodic acid Schiff (PAS) com e sem digestão prévia pela amilase salivar e Alcian Blue (AB) em pH 2,5 e 0,4. Utilizou-se a coloração de Ninhidrina-Schiff para evidenciação de proteínas ricas em radicais -NH 2 . Foram observadas alterações em 34 tubas uterinas. Independente da fase do ciclo estral, as patologias mais comumente encontradas foram os cistos de parede e a metaplasia escamosa. Em menor número foram observados divertículo e adenomiose. A ampola e o infundíbulo foram os segmentos mais afetados. Na fase folicular, o infundíbulo apresentou maior intensidade de secreção de mucossubstâncias neutras e AB 2,5-positivas. Na ampola houve maior intensidade de secreção de mucossubstâncias AB 2,5-positivas, sulfomucinas e de proteínas ricas em radicais -NH 2. As patologias não reduziram significativamente a secreção de mucossubstâncias nas regiões adjacentes em nenhum dos segmentos tubáricos, tanto na fase luteínica quanto na folicular. Nas áreas de metaplasia escamosa do epitélio tubárico e nos cistos de parede houve redução da secreção de mucossubstâncias neutras e ácidas.Palavras-chave: porca, tuba uterina, patologia, histoquímica, fase folicular, fase luteínica (pH 2.5 and 0.4), and Ninhidrina-Schiff stains were used for the histochemical study. Grossly, 34 uterine tubes presented morphological lesions. Wall cysts and squamous metaplasia were the changes found more frequently in both phases. Ampulla and infundibulum were the segments affected more frequently. In the follicular phase, the infundibulum had a higher secretion intensity of neutral mucosubstances, whereas the ampulla and the infundibulum had a higher secretion intensity of AB 2.5-positive mucosubstances. The ampulla had a higher intensity of Recebido para publicação em 11 de março de 2003 Recebido para publicação, após modificações, em 13 de outubro de 2003 ABSTRACT The goal of this study was to describe the anatomopathological and histochemical features of uterine tubes from 62 sexually mature sows, in both follicular and luteal phases. The animals were assigned into four groups as follows: the first (LSP) containing sows in the luteal phase without tube pathology (n=45), the second (LCP) with sows in the luteal phase with tube pathology (n=19), the third (FSP) with sows in the follicular phase without tube pathology (n=45) and the fourth (FCP) with sows in the follicular phase with tube pathology (n=15). PAS, Alcian Blue

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“…In the uterine tubes the epithelium was characterized as simple ciliated columnar resting on a lamina propria of loose connective tissue (Figure 1). Similar observations were made by Sant' Ana [15] who noted that the mucosa of the uterine tube of adult sows is covered by simple columnar epithelium formed essentially by ciliated cells. In another study, Monteiro [9] reported that the mucosa of the uterine tubes of prepubertal gilts presents areas of simple ciliated columnar epithelium.…”

Cyclic Related and Pathological Changes in the Lectin‐binding Sites on the Swine Oviduct

“…In addition, many studies point out that the lesions in the porcine oviduct are important causes of infertility in this species (Nalbandov 1952; Perry and Pomeroy 1956; Einarsson and Gustafsson 1970; Heinonen et al. 1998; Sant'Ana et al. 2004).…”

“…(2003) showed that lesions diagnosed in sow's oviducts do not reduce the epithelial thickness significantly. In another study, the same authors concluded that morphologic alterations of the sow's oviducts do not reduce the secretion of mucosubstances significantly (Sant'Ana et al. 2004).…”

“…After evisceration, sections of the oviduct (isthmus, ampulla and infundibulum), were collected for histological and histochemical analysis. The morphological alterations observed in the oviducts used in this study were described in a previous paper (Sant'Ana et al. 2004).…”

Histomorphometric comparison of left and right oviduct structure from alpaca (Vicugna pacos)

“…The percentage distribution of ciliated and secretory cells and the secretion pattern of the oviductal mucosa were calculated according to reports by Sant'Ana et al (2004), Gonella‐Diaza et al (2017) and Machado‐Neves et al (2019). The identification of the ciliated cells was based on the presence of cilia and the nucleus in the apical position and the secretory cells with the presence of cytoplasmic protuberances, PAS positive granules and the nucleus in the basal position.…”