Ocorrência de Staphylococcus sp. em água utilizada em propriedades leiteiras do Estado de São Paulo

Amaral, Luiz Augusto do; Júnior, Oswaldo Durival Rossi; Ferreira, Francisco; Barros, Ludmilla Santana Soares e

doi:10.1590/s0102-09352003000500017

Cited by 9 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…518/2004, from the Ministry of Health; however, the samples showed the presence of TC and FC, and may have been a likely source of contamination by TC and FC of teats and milking utensils, and consequently of the milk produced. The result foud results corroborate the statement of Amaral et al [3], which emphasized the importance of the water used in order to obtain products of good microbiological quality; because, according to the author, the water used in production has great influence on the contamination of the milk, and, being a vehicle for transmission of pathogens, must have characteristics of potability. Through observation of the practices adopted by the producers, negligence was noted on the judicious use of cleaning systems and sanitizing of equipment, especially in relation to concentrations of chemicals used, as well as the temperature and circulation time of the solutions.…”

Section: Resultssupporting

confidence: 84%

“…Due to its characteristics, milk deserves special attention in its production, processing, marketing and consumption. Several factors, such as the health of the herd, sterility of the cleaning equipment and utensils used to obtain it, the health conditions of the milking place, the excretion from the udder of an infected animal and quality of water used on the farm, may influence the microbiological quality of milk prod ucts [3][4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Nádia¹,

Scapin²,

Oro³

et al. 2012

FPH

View full text Add to dashboard Cite

Milk is a food that inherently favors microbial growth and due to its characteristics several precautions must be taken to prevent contamination in its production, processing, marketing and consumption, which are routinely subject to changes. The aim of this study was to evaluate microbiological contamination in milk produced at two farms in the Far West of Santa Catarina, before and after the application of Good Manufacturing Practices (GMP). Initially, samples of the milk, surfaces of equipment and utensils for milking, the teats of animals, disinfectants, and water were tested. Next, we conducted training of the farmers in microbiological analysis of milk samples. The analyses included counts of mesophilic aerobes (MA), Staphylococcus coagulase positive (SA), total coliform (TC), and thermotolerant (FC). The methods used for analyses were those described by the Regulation number 62 of August 26th, 2003 published by the Brazilian Ministry of Agriculture and Food Supply (MAPA) that follows methods recommended by the Compendium of Methods for the Microbiological Examination of Foods-APHA. The mean values for MA, SA, TC, and FC in milk obtained before and after the training were, respectively: 4.88 and 3.69 log colony-forming-unit (CFU)/ ml, 3.04 and 2.37 log CFU / ml, 61.19 Most probable number (MPN) and 17.89 MPN/ ml, and 40.26 and 8.71 MPN/ ml. Thus, according to these results, including training in GMP can improve the quality of milk, with immediate results for MA, TC, and CF. But, beyond the procedures employed, the control and prevention of mastitis could help to avoid contamination by SA.

show abstract

Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Nádia¹,

Scapin²,

Oro³

et al. 2012

FPH

View full text Add to dashboard Cite

show abstract

“…A água também se destaca como via de transmissão de agentes causais de mastite (Amaral et al 2003). O risco da mastite por Staphylococcus sp.…”

Section: Resultsunclassified

Caracterização da mastite subclínica em caprinos produzidos em sistema orgânico no Rio Grande do Sul

et al. 2009

View full text Add to dashboard Cite

774Pesq. Vet. Bras. 29(9):774-778, setembro 2009 RESUMO.-O leite e derivados são reconhecidos como veículos de patógenos para humanos, secundário a contaminação pós-ordenha ou de infecções do próprio animal, particularmente na mastite. Foi estudada a ocorrên-cia de mastite e aspectos do manejo em cabras de três propriedades criadas em sistema orgânico. O exame clí-nico da glândula mamária de 64 cabras em diferentes períodos de lactação, não acusou a presença de mastite clínica. Entretanto, o Califórnia Mastitis Test (CMT) identificou 54 (22,7%) metades mamárias reagentes (+ ou ++). Foram colhidas 238 amostras de leite, das quais houve isolamento bacteriano em 37 (15,6%). Em apenas oito amostras houve coincidência entre o isolamento bacteriano e o resultado do CMT, indicando sensibilidade de 21,6% para este teste no diagnóstico de mastite subclíni-ca em caprinos. Staphylococcus coagulase negativa (SCN) foi o microrganismo mais freqüente (83,8%). O teste de sensibilidade microbiana in vitro revelou resistência das linhagens de SCN ao cotrimoxazol (50%), ampicilina (48,1%), nitrofurantoína (7,7%), cefaclor (7,14%) e oxacilina (3,85%). Cefalotina, gentamicina, neomicina, estreptomicina e tetraciclina foram os antimicrobianos mais efetivos frente aos isolados. Não se evidenciou relação en- Milk and derivates are recognized as vehicle of different pathogens for humans. These micoorganisms may manifest as post-milking contamination or as in infections in the animal itself, particularly in mastitis. The aim of present study was characterizes the occurrence of mastitis and management aspects in dairy-goats raised in three farms of organic milk. The correlation between mastitis and goat husbandry was also investigated. The clinical exam of the mammary glands of 64 dairy-goats did not reveal the presence of clinical mastitis. Nevertheless, the California Mastitis Test (CMT) identified 54 (22.7%) reactive mammary halves (+ or ++). From 238 milk samples collected, 37 (15.6%) were positive for bacterial isolates. Coincident positive results for bacterial isolate tests and CMT were observed in only eight samples, which indicate a sensitivity index of 21.6% for the caprine subclinical mastitis diagnosis test. Coagulase-negative staphylococci (CNS) were identified as the most frequent pathogen in milk samples (83.8%). The in vitro sensitivity test revealed CNS strains resistant to cotrimoxazole (50%), ampicillin (48.1%), nitrofurantoine (7.7%), cefaclor (7.14%), and oxacilline (3.85%). Cefalotine, gentamicin, neomycin, streptomycin, and tetracycline were the most effective drugs. No correlation was observed between the occurrence of subclinical mastitis and race, lactation period, drinking water quality in farms, or milking system as adopted in the present study.

show abstract

“…Amaral et al (2003) emphasize the importance of infection in the mammary gland as a result of CNS, which may be responsible for longterm processes that compromise the composition and milk production. In similar studies, the authors found CNS as the most prevalent agent (FREITAS et al, 2005;TENHAGEN et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Bovine mastitis: prevalence and antimicrobial susceptibility profile and detection of genes associated with biofilm formation in Staphylococcus aureus

Casanova

Appio

Kohl

et al. 2016

SCA

View full text Add to dashboard Cite

Brazil currently ranks as one of the world leaders in food production and exportation. This scenario encourages the development of animal and plant health programs to ensure the production of safe food, helping the country to become an international provider of food for excellence. However, some health problems in dairy production, such as mastitis, have garnered increasing concern. This study aimed to estimate the prevalence of bovine mastitis in select properties located in the western Santa Catarina region, to assess the susceptibility profile to antimicrobial agents used for treatment and to check for the presence of genes (icaA and icaD) associated with biofilm formation in Staphylococcus aureus. In 148 milk samples collected, 72.97% had bacterial growth (n = 108). Among the isolated microorganisms, 21.62% (n = 32) were classified as Staphylococcus aureus, 18.91% (n = 28) as Staphylococcus sp. coagulase negative, 7.43% (n = 11) as Corynebacterium sp., 6.76% (n = 10) as Staphylococcus sp. coagulase positive, 5.41% (n = 8) as Nocardia sp. and 12.83% (n = 19) classified in different bacterial genera. Among the isolates submitted for antimicrobial susceptibility testing, it was observed that 8.95% (n = 6/67) had resistance to amoxicillin, 8.04% (n = 7/87) to ampicillin, 5.88% (n = 5/85) to cephalothin, 3.40% (n = 3/88) to ceftiofur and enrofloxacin, 20.45% (n = 18/88) to streptomycin, 17.04% (n = 15/88) to gentamicin and lincomycin, 31.81% (n = 28/88) to neomycin, 14.94% (n = 13/87) to penicillin and 25% (n = 22/88) to tetracycline. Staphylococcus sp. coagulase negative isolates showed higher multidrug resistance when compared to those of S. aureus and Staphylococcus sp. coagulase positive. Thirty-one strains of S. aureus isolates were genotypically tested by polymerase chain reaction (PCR), yielding a positive result for the icaA gene in 83.87% of the samples, 80.64% positive for icaD and 74.19% of these showed both genes. The results reinforce the importance of controlling this disease by adopting strict hygiene protocols and disinfection of equipment used for milking and the prescription of appropriate therapies to clinical and subclinical cases of mastitis, as well as evaluating not only the susceptibility profile of isolates but also the biofilm formation capacity. ResumoO Brasil situa-se hoje como um dos líderes mundiais em produção e exportação de alimentos. Este cenário incita a elaboração de programas de sanidade animal e vegetal que garantam a produção de alimentos seguros, contribuindo para que o país se torne um fornecedor internacional de alimentos por excelência. Entretanto, alguns problemas sanitários na produção leiteira, como a mastite, têm acarretado crescentes preocupações. O presente estudo teve como objetivo estimar a prevalência de mastite bovina em algumas propriedades localizadas na região Oeste de Santa Catarina, avaliar o perfil de suscetibilidade aos antimicrobianos empregados para tratamento e verificar a presença de genes (icaA e icaD) associados à formação de biofilmes em ...

show abstract

Ocorrência de Staphylococcus sp. em água utilizada em propriedades leiteiras do Estado de São Paulo

Cited by 9 publications

References 8 publications

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Evaluation of Microbiological Quality of Raw Milk Produced at Two Properties in the Far West of Santa Catarina, Brasil

Caracterização da mastite subclínica em caprinos produzidos em sistema orgânico no Rio Grande do Sul

Bovine mastitis: prevalence and antimicrobial susceptibility profile and detection of genes associated with biofilm formation in Staphylococcus aureus

Contact Info

Product

Resources

About