Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles

Abstract

The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.

Comparison between total intravenous anesthesia with propofol and intermittent bolus of tiletamine-zolazepam in capuchin monkey (Sapajus apella)

“…Capuchin monkeys (Sapajus apella) are important nonhuman primates for biomedical research and the best neotropical primates at adapting to captivity (Domingues et al 2003). Anesthesia for clinical and surgical procedures in captive primates is often required in zoos and research centers.…”

Efeito Do Intervalo De Secção Sobre O Isolamento Mecânico De Folículos Pré-Antrais De Cadelas

“…Em cadelas, Durrant et al (1998) não encontraram essa associação com peso ovariano e fase reprodutiva, no entanto isolaram menos folículos de fêmeas jovens (≤ 6 meses) em comparação as de meia idade (entre 7 e 48 meses). Domingues et al (2003) isolaram mecanicamente folículos pré-antrais de ovários de primatas (Cebus apella), testando espessuras de corte de 250, 500, 750 e 1000 µm e reportaram que o número de FOPA obtidos no corte de 500 µm (300830) foi estatisticamente superior aos demais. Estudo com ovários bovinos seccionados mecanicamente em oito espessuras de corte (25,50,75,100,125,150, 175 e 200 µm) demonstrou que com o intervalo de 125 µm obteve-se o maior número (25632) de folículos isolados (Lucci et al, 2002).…”

“…As possibilidades do emprego dos folículos ovarianos pré-antrais em sistemas de crescimento, maturação e fecundação in vitro abre um leque de novas alternativas para a seleção e melhoramento genético animal (Figueiredo et al, 1993;Telfer et al, 1999). (Domingues et al, 2003). Todavia, não existem dados de literatura avaliando a melhor espessura das fatias de ovário para o isolamento de folículos pré-antrais em cadelas.…”

Vitamin E-analog Trolox prevents endoplasmic reticulum stress in frozen-thawed ovarian tissue of capuchin monkey (Sapajus apella)

“…Capuchin pre-antral follicles were mechanically isolated from fresh and freeze-thawed ovarian fragments according to the procedure described by Domingues et al (2003). Pre-antral follicles smaller than 100 μm were collected under a dissecting stereomicroscope (SZ-STS, Olympus, Tokyo, Japan) and transferred to TCM-199.…”