Uso de inóculo padronizado de Anaplasma marginale e da quimioprofilaxia no controle da anaplasmose bovina

Ribeiro, Múcio Flávio Barbosa; Facury-Filho, Elias Jorge; Passos, Lygia Maria Friche; Saturnino, H.M.; Malacco, M.A.F.

doi:10.1590/s0102-09352003000100004

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Ixodid ticks are the biological vectors of A. marginale, while mechanical transmission can occur through fly bites and reuse of needles [5]. The one-host tick R. microplus is estimated to be the main vector of A. marginale in Brazil [24,25]. Once an animal is exposed to this pathogen, acute infection develops, which is characterized by fever, high levels of bacteremia, anemia, weakness, reduced growth and milk production, miscarriage, and in some cases death [26].…”

Section: The Ecology Of Ticks As a Framework For Successful And Sustamentioning

confidence: 99%

Towards an Effective, Rational and Sustainable Approach for the Control of Cattle Ticks in the Neotropics

et al. 2019

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Ticks and transmitted pathogens constitute a major burden for cattle industry in the Neotropics. To address this limitation, the Spanish Ibero-American Program of Science and Technology in Development office (CYTED) supported from 2018 a network of scientists named “LaGar” (CYTED code 118RT0542) aimed at optimizing the control strategies of cattle ticks in the neotropical region. As part of network activities, a meeting and course were organized on 4–8 November 2019 in Querétaro, Mexico to address the objective of developing the infrastructure necessary for an effective, sustainable (i.e., combination of efficacious acaricides with anti-tick vaccines) and rational (i.e., considering tick ecology, seasonal dynamics and cattle-wildlife interactions) control of cattle tick infestations and transmitted pathogens. The course was focused on scientists, students, cattle holders and producers and pharmaceutical/industry representatives. In this way the course addressed the different views presented by participants with the conclusion of producing a research-driven combination of different interventions for the control of tick tick-borne diseases.

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Section: The Ecology Of Ticks As a Framework For Successful And Sustamentioning

confidence: 99%

Towards an Effective, Rational and Sustainable Approach for the Control of Cattle Ticks in the Neotropics

et al. 2019

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“…In areas of enzootic instability, administration of antibiotics or inoculation of calves with live vaccine has been used (RIBEIRO et al, 2003). However, adult cattle with no prior exposure are highly susceptible to developing clinical disease, even when inoculated with a sample of low virulence.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture

Lasmar

Carvalho

Filho

et al. 2012

Rev. Bras. Parasitol. Vet.

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The protective efficacy of an inactivated vaccine from Anaplasma marginale that was cultured in tick cells (IDE8) for use against bovine anaplasmosis was evaluated. Five calves (Group 1) were inoculated subcutaneously, at 21-day intervals, with three doses of vaccine containing 3 × 10(9) A. marginale initial bodies. Five control calves received saline solution alone (Group 2). Thirty-two days after the final inoculation, all the calves were challenged with approximately 3 × 10(5) erythrocytes infected with A. marginale high-virulence isolate (UFMG2). The Group 1 calves seroconverted 14 days after the second dose of vaccine. After the challenge, all the animals showed patent rickettsemia. There was no significant difference (p > 0.05) between the Group 1 and 2 calves during the incubation period, patency period or convalescence period. All the animals required treatment to prevent death. The results suggest that the inactivated vaccine from A. marginale produced in IDE8 induced seroconversion in calves, but was not effective for preventing anaplasmosis induced by the UFMG2 isolate under the conditions of this experiment.

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A tick cell line as a powerful tool to screen the antimicrobial susceptibility of the tick-borne pathogen Anaplasma marginale

Alonso

Ventura

Esteves

et al. 2020

Experimental Parasitology

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Background Anaplasma marginale is the causative agent of the severe bovine anaplasmosis. The tick Rhipicephalus microplus is one of the main vectors of A. marginale in tropical and subtropical regions of world. After the tick bite, the bacterium invades and proliferates within the bovine erythrocytes, causing anemia and impairing milk production and weight gain. In addition, infection can cause abortion and high mortality in areas of enzootic instability. The immunization with live and inactivated vaccines are employed to the control acute bovine anaplasmosis. However, they do not prevent persistent infection. Therefore, infected animals, even if immunized, are reservoirs of the bacterium and contribute to the dissemination of the disease. Antimicrobials are also largely employed for the prophylaxis of bovine anaplasmosis. However, they are often used in subtlethal doses, what can select pre-existing resistant bacteria and induce genetic or phenotypic variations. Therefore, the standardization of an in vitro assay to evaluate the susceptibility of A. marginale strains to different antimicrobials is important to allow the prescription of the more effective treatment, preventing both the selection and spread of resistant strains. Results Initially the antimicrobial susceptibility of two eld isolates of A. marginale (Jaboticabal and Palmeira) infecting bovines was evaluated. The least susceptible strain (Jaboticabal) was used for the standardization of an antimicrobial assay using a culture of Ixodes scapularis-derived tick cell line, ISE6.Results showed that enro oxacin (ENRO) at 0.25, 1 or 4 μg/mL and oxytetracycline (OTC) at 4 or 16 μg/mL are the most e cient treatments, followed by OTC at 1 μg/mL and imidocarb dipropionate (IMD) at 1 or 4 μg/mL. Conclusion In the current study, we present a new in vitro assay using a tick cell line to evaluate the susceptibility of A. marginale to antimicrobials. The maintenance of such culture is much easier than the maintenance of bovine erythrocyte culture, which depends on continuous cell replacement. This assay may be used to guide cattle farmers to the correct use of antimicrobials. The choice of the most suitable antimicrobial is essential to eliminate persistent infections, preventing the spread of resistant strains and helping in the control of bovine anaplasmosis.

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Uso de inóculo padronizado de Anaplasma marginale e da quimioprofilaxia no controle da anaplasmose bovina

Cited by 3 publications

References 3 publications

Towards an Effective, Rational and Sustainable Approach for the Control of Cattle Ticks in the Neotropics

Towards an Effective, Rational and Sustainable Approach for the Control of Cattle Ticks in the Neotropics

Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture

A tick cell line as a powerful tool to screen the antimicrobial susceptibility of the tick-borne pathogen Anaplasma marginale

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