Hemograma e proteinograma plasmático de eqüinos hígidos e de eqüinos acometidos por abdômem agudo, antes e após laparotomia

Fagliari, José Jurandir; Silva, S.L.

doi:10.1590/s0102-09352002000600001

Cited by 19 publications

(9 citation statements)

References 9 publications

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“…The electrophoresis technique with sodium dodecil sulphate (SDS-PAGE) is simple, less expensive, and is enable to visualize proteins with extremely low concentrations as well the identification of 20 to 30 proteins. Using this technique, Fagliari et al (1997) identified 19 proteins in the serum proteinogram in equines with laminitis and Fagliari et al (2002) studied the serum proteinogram of equine with acute abdomen. The aim of the present study was to evaluate the results of hemogram and serum protein concentrations in queens submitted to videolaparoscopic or conventional ovariectomy.…”

Section: Introductionmentioning

confidence: 99%

Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy

Alves

Ribeiro

Filippo

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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Thirty health queens were submitted to ovariectomy by conventional technique or by videolaparoscopy. In order to study the intensity of inflammatory response by means of acute phase protein analysis and white blood cell count, serum samples were taken before and until 144 hours after the surgical procedures. The protein concentrations that were significantly increased 24 hours after surgical procedures were: ceruloplasmin, hemopexin, haptoglobin, and 1 -acid glycoprotein, 69.8%, 103.5%, 117.3%, and 199.0%, respectively, for conventional ovariectomy; and 22.3%, 46.1%, 79.8%, and 74.6%, respectively, for laparoscopic ovariectomy. Therefore, inflammatory response was more intense in queens submitted to conventional ovariectomy. Results indicate that the increase or decrease in acute phase proteins, as well as in white blood cells count, may be useful in the evaluation of inflammatory response induced by these surgical procedures. 1 -glicoproteína ácida, 69,8%, 103,5%, 117,3% e 199,0%, respectivamente, para ovariectomia convencional, e 22,3%, 46,1%, 79,8% e 74,6%, respectivamente, para

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Section: Introductionmentioning

confidence: 99%

Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy

Alves

Ribeiro

Filippo

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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“…Separation of the protein fractions was achieved by SDS-PAGE, as described by Laemmli (1970) and Fagliari & Silva (2002). Protein concentrations were measured using a computerized densitometer.…”

Section: Methodsmentioning

confidence: 99%

Effects of intramammary infection on whey proteinograms of sheep during lactation

et al. 2015

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RESUMO.-[Efeitos da infecção intramamária no proteinograma de ovinos durante a lactação.] Este estudo teve como objetivo identificar potenciais biomarcadores de infecção na glândula mamária de ovelhas Santa Inês. Foram monitorados rebanhos comerciais de ovinos, submetidos ao mesmo manejo higiênico-sanitário e nutricional, criados em sistemas de produção semi-intensivo durante as várias fases de lactação e avaliados aos 15, 30, 60 The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), α-lactalbumin, β-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and α-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and α-lactoalbumin. The most significant changes were observed in α-1-acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, α-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep. CMT). O leite foi coletado para aná-lise bacteriana e análise de proteínas. Foi realizada a cultura bacteriana e a caracterização bioquímica das amostras. Foram selecionadas 42 amostras de leite provenientes de glândulas sadias (CMT negativo e teste bacteriológico negativo) e 43 amostras de leite de glândulas infectadas (CMT positivo e teste bacteriológico positivo). O soro lácteo foi obtido por meio da adição de renina. A proteína total no soro lácteo foi mensurada utilizando o método do biureto e a identificação das proteínas empregando-se eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE), permitindo a quantificação de nove proteínas no soro lácteo das glândulas sadias: albumina, lactoferrina, IgA...

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“…A separação das proteínas lácteas foi realizada utilizando--se a técnica de eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE), conforme descrito por Laemmli (1970) e Fagliari & Silva (2002). Para a identificação das proteínas do soro lácteo foi empregada como referência solução marcadora 12 com diversos pesos moleculares (6.500-200.000 Da) e as proteínas purificadas lactoferrina, β-lactoglobulina e a α-lactoalbumina bovinas.…”

Section: Methodsunclassified

“…A eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE) é uma técnica também empregada na separação das proteínas do leite, permitindo a identificação de várias frações protéicas, bem como sua quantificação, propiciando a visualização de 20 a 30 proteínas com pesos moleculares que variam entre 24.000 a 340.000 dáltons (Da) (Fagliari & Silva 2002, Patel et al 2007. A caracterização, tanto sob o ponto de vista qualitativo, como quantitativo das proteínas do soro lácteo, está documentada na vaca (Sant'ana 2004, Raimondo et al 2009, Rocha et al 2009) e na cabra (Raimondo et al 2011), no entanto em ovelhas a literatura é bastante escassa, particularmente se tratando do acompanhamento em diferentes estágios da lactação.…”

Section: Introductionunclassified

Proteinograma do soro lácteo de ovelhas da raça Santa Inês em diferentes fases de lactação

et al. 2013

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Pesq. Vet. Bras. 33(6) This study aimed to evaluate the influence of lactation phases on the proteinogram of whey protein in Santa Inês ewes. Ewes were accompanied in a semi-intensive system using the same sanitary and nutritional management evaluated at 15, 30, 60 and 90 days postpartum (end of weaning and lactation). Clinical examination of the mammary gland was carried out through and bacteriological culture. The screening of the material resulted in 44 milk samples of healthy glands concurrent negative by CMT and bacteriological culture exam. For obtaining the whey protein renin solution was used. The whey was fractionated into aliquots and kept in the -80C freezer to later separation of protein fractions. For determination of total protein of whey protein was employed the biuret, observing the linearity of the test. Separation of protein fractions was performed, using polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). Eigth protein were observed including lactoferrin, serum albumin, IgA, IgG (heavy chain IgG (IgG CP), light chain IgG (IgG CL), β-lactoglobulin, α-lactalbumin and proteins identified as PM 15000 and PM 29000. No significant difference was observed at different stages of lactation in the following protein: IgA (P>0.3895), lactoferrin (P>0.1611), PM 29000 (P>0.4879), α-lactalbumin (P>0.0799) and PM15000 (P>0.4494). In total protein (P<0.0022), albumin protein (P<0.0377) and IgG (P<0.0354) it was observed a significant variation in the first moments of observations, in the β-lactoglobulin protein (P<0.0005) there was significant variation with reduction of 15 to 30 days postpartum with progressive elevation until the last stage of lactation (90 days postpartum). The SDS-PAGE technique allowed the quantification of eigth whey proteins in health ewes. The protein fractions identified reflect the profile of whey to ovine species, with influence of stages of lactation in albumin, IgG and β-lactoglobulin.INDEX TERMS: Proteinogram, whey protein, postpartum, lactation, small ruminants, milk, SDS-PAGE.

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Hemograma e proteinograma plasmático de eqüinos hígidos e de eqüinos acometidos por abdômem agudo, antes e após laparotomia

Cited by 19 publications

References 9 publications

Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy

Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy

Effects of intramammary infection on whey proteinograms of sheep during lactation

Proteinograma do soro lácteo de ovelhas da raça Santa Inês em diferentes fases de lactação

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