Isolamento de Mycobacterium bovis em cão

Mota, Pedro; Lobato, Francisco Carlos Faria; Assis, Ronnie Antunes de; Lage, Andrey Pereira; Parreiras, Patrícia Martins

doi:10.1590/s0102-09352001000400003

Cited by 7 publications

(9 citation statements)

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“…Dados similares aos observados por Gathogo et al (2012), que inspecionaram 929 carcaças em dois abatedouros no Kênia e observaram que as lesões distribuídas nas carcaças estavam localizadas nos gânglios linfáticos brônquicos e mediastinais, parênquima pulmonar, parênquima hepático e linfonodos localizados em outros tecidos. No exame bacteriológico dos fragmentos de lesões provenientes de bovinos abatidos, os resultados foram compatíveis com a literatura, sendo relatado crescimento das primeiras colônias de M. bovis em meio Stonebrink-leslie de 21 dias (Cousins et al 1989), 36 dias (Corner 1994) e 28 dias (Mota et al 2001). Segundo Corner (1994), o diagnóstico bacteriológico é considerado "padrão ouro", apesar de requerer um longo período para o crescimento das primeiras colônias e identificação da espécie.…”

Section: Discussionunclassified

Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

et al. 2014

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Pesq. Vet. Bras. 34(2):103-108, fevereiro 2014 103 RESUMO.-O objetivo do presente trabalho foi utilizar mé-todos bacteriológicos e moleculares para a identificação do Mycobacterium bovis em lesões observadas em carcaças de bovinos durante a inspeção post mortem de rotina em matadouros-frigoríficos com serviço de inspeção oficial. Foi acompanhado o abate e a inspeção de 825.394 bovinos, sadios ao exame ante mortem pelo serviço de inspeção oficial em dez matadouros-frigoríficos do estado da Bahia. Carcaça de 180 bovinos apresentaram lesões sugestivas de tuberculose e por outras linfadenites. No isolamento bacteriano, 25 amostras apresentaram crescimento disgônico de colônias de coloração creme-amareladas em meio de cultura Stonebrink-Leslie. Desses isolados, 14 foram identifi- Múltiplas estirpes de isolados de Mycobacterium bovisidentificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos The aim of this study was to use bacteriological and molecular methods to identify Mycobacterium bovis in lesions observed in cattle carcasses during routine post-mortem inspection in slaughterhouses with official inspection service. It was accompanied the slaughter and inspection of 825,394 cattle, healthy ante mortem examination by the official inspection service in ten slaughterhouses in the state of Bahia. Carcasses of 180 cattle presented lesions suggestive of tuberculosis and other lymphadenitis. In bacterial isolation, 25 samples showed dysgonic growth of colonies of creamy-yellow in medium-Stonebrink Leslie. From these isolates, 14 were identified as M. bovis and the multiplex PCR technique spoligotyping was discriminated against eight different spoligotypes of M. bovis, seven previously described in the literature and a new spoligotypes without former description. The major spoligotypes was SB0121, with five samples which has been described in Brazil and other countries, followed by two clusters, SB295 and SB1055, with two isolates each. The SB1145 and SB1648 spoligotypes were reported only in Brazil and Denmark, respectively. The spoligotypes SB140 has been found in Brazil, Argentina, Uruguay and Paraguay. These results demonstrate that the spoligotypes obtained are shared, so far, among Brazilian states and among Latin America and Europe. Thus, molecular discrimination of isolates of M. bovis by Spoligotyping constitutes a tool for epidemiological studies of bovine tuberculosis in the state of Bahia. INDEX TERMS: Mycobacterium bovis, mycobacteria, molecular epidemiology, spoligotyping, tuberculosis, zoonosis, cattle. (Brasil 2006, Heinemann et al. 2008.A tuberculose no homem é causada principalmente pelo Mycobacterium tuberculosis, mas a doença causada por M. bovis tem sido reportada e representa um risco para a saúde pública (Parreiras et al. 2012, Reyes et al. 2012. Em 2011, 8,7 milhões de pessoas adoeceram e 1,4 milhões de óbitos anuais foi determinado pela tuberculose. A maioria dos óbitos ocorre em países de baixa e média renda e está entre as três principais causas de mortes em mulheres ent...

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Section: Discussionunclassified

Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

et al. 2014

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“…However, canine TB cases remain largely misdiagnosed or undetected in these countries. [13][14][15] Tuberculosis in naturally infected companion animals is most commonly a subclinical disease. In dogs that develop clinical signs, the most common primary sites of TB infections are the lungs and the pulmonary lymph tract.…”

Section: 11mentioning

confidence: 99%

“…15 In companion animals, TB is considered an anthropozoonosis. The inhalation and consumption of sputum or contaminated food appears to be the major sources of transmission of M. tuberculosis from infected humans to dogs.…”

Section: 17mentioning

confidence: 99%

Disseminated Mycobacterium tuberculosis Infection in a Dog

Martinho

Franco²,

Ribeiro³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. An uncommon disseminated Mycobacterium tuberculosis infection is described in a 12-year-old female dog presenting with fever, dyspnea, cough, weight loss, lymphadenopathy, melena, epistaxis, and emesis. The dog had a history of close contact with its owner, who died of pulmonary tuberculosis. Radiographic examination revealed diffuse radio-opaque images in both lung lobes, diffuse visible masses in abdominal organs, and hilar and mesenteric lymphadenopathy. Bronchial washing samples and feces were negative for acid-fast organisms. Polymerase chain reaction (PCR)-based species identification of bronchial washing samples, feces, and urine revealed M. tuberculosis using PCR-restriction enzyme pattern analysis-PRA. Because of public health concerns, which were worsened by the physical condition of the dog, euthanasia of the animal was recommended. Rough and tough colonies suggestive of M. tuberculosis were observed after microbiological culture of lung, liver, spleen, heart, and lymph node fragments in Lö wenstein-Jensen and Stonebrink media. The PRA analysis enabled diagnosis of M. tuberculosis strains isolated from organs.

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“…Although the disease is generally observed in humans and animals, infections in dogs are rare. To the best of our knowledge, only a few studies, thus far, have reported the incidence of TB among dogs 2, 3, 4, 5 6 …”

Section: Introductionmentioning

confidence: 99%

“…Even animals presenting extensive lesions may remain asymptomatic for a long period of time 8 . The clinical signs are not pathognomonic for the infection and can be easily confused with those of other diseases 3 . In addition, the tuberculin test is not routinely performed in dogs 9 .…”

Section: Introductionmentioning

confidence: 99%

Infection by Mycobacterium bovis in a dog from Brazil

Rocha

Figueiredo

Rosales

et al. 2017

Brazilian Journal of Microbiology

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Tuberculosis (TB) is a chronic disease caused by bacteria belonging to the Mycobacterium tuberculosis complex (MtbC). This disease rarely affects dogs. Canine infections are usually caused by M. tuberculosis. Mycobacterium bovis infections are rare in dogs and associated with consumption of raw milk or contaminated products. Here, we report a Boxer dog who had a M. bovis infection and was admitted to a Brazilian veterinary hospital with a presumptive diagnosis of chronic ehrlichiosis. Despite receiving treatment for chronic ehrlichiosis, it progressed to death. TB was diagnosed during post-mortem examinations using histopathological analysis. Ziehl-Neelsen staining revealed acid-fast bacilli in the kidneys, liver, mesentery, and a mass adhered to the liver. Further, PCR-restriction analysis was performed to identify mycobacteria in the samples. A restriction profile compatible with MtbC was found in the lungs. In addition, PCR-based MtbC typing deletions at different loci of chromosome 9 enabled the identification of M. bovis in the lungs. Therefore, it is very essential to perform differential diagnosis of TB in dogs with non-specific clinical signs and who do not respond to treatment, particularly those who had been in contact with TB-infected cattle or owners. Further, we highlight the use of molecular methods for the identification of bacilli, improving the diagnosis and aiding epidemiological studies.

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Isolamento de Mycobacterium bovis em cão

Cited by 7 publications

References 1 publication

Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

Disseminated Mycobacterium tuberculosis Infection in a Dog

Infection by Mycobacterium bovis in a dog from Brazil

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