A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies

Cassali, Geovanni Dantas; Silva, Pedro; Rêma, Alexandra; Gärtner, Fátima; Gobbi, Helenice; Tafuri, Washington Luiz; Schmitt, Fernando

doi:10.1590/s0102-09352001000300010

Cited by 8 publications

(5 citation statements)

References 3 publications

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“…Immunohistochemical techniques were performed using an automated system which, according to Cassali et al [26], results in improved quality, reproducibility, speed, and standardization. …”

Section: Discussionmentioning

confidence: 99%

“…Considering feline mammary tumours as promising models to achieve a better understanding of human breast carcinogenesis, P-cadherin was studied as a prognostic marker in these neoplasms. Immunohistochemical techniques were performed using an automated system which, according to Cassali et al [ 26 ], results in improved quality, reproducibility, speed, and standardization.…”

Section: Discussionmentioning

confidence: 99%

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P-Cadherin Expression in Feline Mammary Tissues

Figueira

Teodósio

Carvalheira

et al. 2012

Veterinary Medicine International

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The search for molecular markers in the feline mammary gland, namely, the adhesion molecules belonging to the cadherin family, is useful in the understanding of the development of mammary carcinomas in felines and humans. To study P-cadherin expression in the feline mammary gland, 61 samples of normal (n = 4), hyperplastic (n = 12), and neoplastic (n = 45) feline mammary tissues were examined. In both normal and hyperplastic mammary tissues as well as in benign tumours, P-cadherin immunolabelling was restricted to myoepithelial cells. In malignant tumours, however, there was an aberrant epithelial P-cadherin immunoexpression in 64.1% (n = 25) of cases, with a membranous and/or cytoplasmic pattern of distribution. A statistically significant relationship was seen between epithelial P-cadherin expression and malignant mammary lesions (P = 0.0001). In malignant mammary tumours, there was likewise a statistically significant relationship between aberrant P-cadherin immunoexpression and histological grade (P = 0.0132). Aberrant epithelial P-cadherin expression seems to be related to malignancy in the feline mammary gland. To confirm the results of this investigation, further studies with larger samples and follow-up studies are warranted.

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“…Immunohistochemical techniques were performed using an automated system which, according to Cassali et al [26], results in improved quality, reproducibility, speed, and standardization. …”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

P-Cadherin Expression in Feline Mammary Tissues

Figueira

Teodósio

Carvalheira

et al. 2012

Veterinary Medicine International

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“…Heat-induced antigen retrieval using the Dako Antigen Retrieval Solution (Dako) was previously performed in a wet bath for 20 min. The reagents were applied using an automatic immunohistochemical system (Lab Vision Autostainer, Model LV-1) as described elsewhere (23). As a positive control we used human breast cancer tissue known to express c-erbB-2 and MIB-1.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

c-erbB-2 expression and nuclear pleomorphism in canine mammary tumors

Dutra

Granja

Schmitt

et al. 2004

Braz J Med Biol Res

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The objective of the present investigation was to study the expression of c-erbB-2 and MIB-1 and try to associate them with morphological features of the cell such as nuclear pleomorphism, mitotic count and histological grade in a series of 70 canine mammary gland tumors, 22 of them benign and 48 malignant. Tumors were collected at the Veterinary Hospital of UFMG (Brazil) and the Veterinary Faculty of Porto University (Portugal). c-erbB-2 expression was determined according to the guidelines provided by the manufacturer of the HercepTest system and nuclear pleomorphism, mitotic count and histological grade according the Elston and Ellis grading system. The HercepTest is the FDA-approved in vitro diagnostic test marketed by Dako. It is a semi-quantitative immunohistochemical assay used to determine overexpression of HER2 protein (human epidermal growth factor receptor) in breast cancer tissue. MIB-1 expression was also evaluated in 28 malignant tumors. Seventeen (35.4%) of the malignant tumors were positive for c-erbB-2 expression, which was positively associated with nuclear pleomorphism (P < 0.0001), histological grade (P = 0.0017) and mitotic count (P < 0.05). Nuclear pleomorphism also showed a positive association with MIB-1 index (P < 0.0001). These results suggest that some of the biological and morphological characteristics of the tumor are associated in canine mammary gland tumors, as also reported for human breast cancer. It was also possible to show that the immunoexpression of c-erbB-2 can be a factor in mammary carcinogenesis. This fact opens the possibility of using anti-c-erbB-2 antibodies in the treatment of canine mammary tumors.

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“…The histopathological features of canine tumors are similar to those of human tumors [22][23][24]. Neoplasms of dogs and humans present several similar features, including histological appearance, tumor genetics, molecular targets, biological behavior, and response to conventional therapies [25,26].…”

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confidence: 96%