Apoptose na modulação da resposta inflamatória aos ovos do Schistosoma mansoni

Tristão, Aparecida; Melo, Alan Lane de; Vasconcelos, A.C.; Grossi, F.M.

doi:10.1590/s0102-09352000000600006

Cited by 2 publications

(7 citation statements)

References 9 publications

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“…Without doubt, major alterations arising out of PTX administration in rodents continue to be those observed previously in the liver (Reis et al , 2001; Xiong et al , 2003; El-Lakkany & Nosseir, 2007; Mati et al , 2010; Rabia et al , 2010). The liver is the organ where the immunological modulation process and its respective alterations on schistosomotic granulomata are more evident (Andrade & Warren, 1964; Carvalho et al , 1986; Silva et al , 2000; Tristão et al , 2000). Hence, data obtained are themselves more suggestive of immunomodulatory than immunosuppressant or just anti-inflammatory activity of PTX in experimental schistosomiasis.…”

Section: Discussionmentioning

confidence: 99%

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Mati

Melo

2012

J. Helminthol.

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AbstractOogram methodology permitted a direct assessment of the fecundity ofSchistosoma mansoniand the passage of parasite eggs from mesenteric vessels into the intestinal lumen in AKR/J mice that had been infected via the intraperitoneal route with 60 cercariae of the trematode and later subjected to short-term subcutaneous treatments with pentoxifylline (PTX). The administration of PTX did not change oviposition kinetics, the individual fecundity of female parasites (as determined by the study of the ratio of second-stageS. mansonieggs per g intestine/number of female parasites recovered from the portal system), nor the number of mature and dead eggs retained in the host tissue, though the drug has known immunomodulatory effects, as shown previously in experimental schistosomiasis. A better appraisal was also carried out, including the study of statistical parameters, concerning the utilization of the ratios of each stage of immature eggs (first to fourth) per g intestine/number of female worms from the portal system. The second-stage eggs had the lowest variability, confirming that the utilization of this stage as an indicator of the individual fecundity of parasite females is indeed viable. In the light of our findings, current uses of oogram methodology are discussed. Moreover, additional consideration is given to data obtained in the present study concerning intraperitoneal infection withS. mansonicercariae in both untreated and treated mice of the AKR/J strain, such as the recovery of mature worms, eggs and free granulomas from the peritoneal cavity of these rodents.

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Section: Discussionmentioning

confidence: 99%

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Mati

Melo

2012

J. Helminthol.

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“…Thus, the mesenteric vasodilatation of the abdominal viscera observed macroscopically in the present study can be related to circulatory system changes caused by this drug. With progression of the pathological process, modifications in the cell constitution and aspect of the granulomas occur, and they become more fibrous and concentric (Raso et al, 1978;Carvalho et al, 1986;Silva et al, 2000;Tristão et al, 2000). However, a slight worm reduction in infected mice after prolonged PTX treatment (8 weeks) has been reported (El-Lakkany & Nosseir, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The less concentric and more irregular granulomas observed can be explained by the reduction in, or inhibition of, the migration of leucocytes to the site of hepatic damage. Tristão et al (2000) demonstrated that the modulation of the inflammatory response around S. mansoni eggs is related to apoptosis. In experimental schistosomiasis, during the cercaria-schistosomulum transformation process, there was a delay in cell adhesion to S. mansoni larvae, possibly due to inhibition of neutrophil migration, suggesting that PTX could have some control over complement system components (A.L.…”

Section: Discussionmentioning

confidence: 99%

“…Macrophages are the most common cells observed in chronic disease, and the eggs progressively acquire a growing fibrous component (Andrade & Warren, 1964;Carvalho et al, 1986;Silva et al, 2000;Tristão et al, 2000). Macrophages are the most common cells observed in chronic disease, and the eggs progressively acquire a growing fibrous component (Andrade & Warren, 1964;Carvalho et al, 1986;Silva et al, 2000;Tristão et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Initially, granulomatous lesions are characterized by polymorphonuclear and lymphocytic cell infiltrates, where the inflammatory reaction is intense. Macrophages are the most common cells observed in chronic disease, and the eggs progressively acquire a growing fibrous component (Andrade & Warren, 1964; Carvalho et al , 1986; Silva et al , 2000; Tristão et al , 2000).…”

Section: Introductionmentioning

confidence: 99%

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Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

2010

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The short-term effects of pentoxifylline (PTX) on granulomatous lesions during Schistosoma mansoni infection in Swiss mice were evaluated. Drug administration was initiated 42 and 140 days post-infection (DPI) for the acute and chronic infection groups, respectively. Treatment was carried out daily with 200 mg/kg (subcutaneous route) of the drug for five consecutive days. Recovery of parasites and tissues was performed at 49 DPI and 147 DPI, respectively. Liver histological analysis showed a decrease in the inflammatory reaction and fibrous content of the granulomas studied, and a significant reduction (P < 0.001) in their mean diameter was observed in the groups of rodents treated with PTX in acute and chronic infection, when compared to their respective control groups. However, no alteration in the number of S. mansoni recovered from the portal system was observed, and egg-laying kinetics was not notably modified by PTX treatment, and the immature stage distribution of S. mansoni eggs showed minor intrinsic variations with no statistical differences in the parameter second-stage/female/g among untreated mice and treated mice in acute and chronic infections, respectively, when evaluated by intestinal oograms. Data obtained indicate probable immunomodulatory effects of PTX in murine schistosomiasis both in acute and chronic infection.

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Apoptose na modulação da resposta inflamatória aos ovos do Schistosoma mansoni

Cited by 2 publications

References 9 publications

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

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