Partial characterization of a Brazilian strain of Aujeszky’s disease virus recovered from a pig with subclinical infection

Abstract

One Brazilian strain of Aujeszky’s disease virus isolated from a piglet in which the disease had not been observed was studied as for its virulence in pigs. The genome of the virus was molecularly analysed as for their restriction endonuclease cleavage pattern. Fifty-day-old non-immune weanlings exposed to this strain showed no disease although the virus was present in their oropharyngeal area for at least three days. All animals developed moderate titers of neutralizing antibody. Based on number of bands and migration rate of restriction fragments the isolate was classified into Herrmann’s type I group. Latent infection was detected in all pigs by PCR. Some variations were detected in the cleavage pattern of the strain ASB Piau when compared to LA031 virulent Brazilian strain, that could be related to differences in the virulence.

Caracterização antigênica e molecular de oito amostras do vírus da doença de Aujeszky isoladas no estado do Rio Grande do Sul em 2003

“…O VDA possui um genoma de DNA de fita dupla com aproximadamente 150 pares de base (Kpb), que codifica mais de 70 proteínas (Mettenleiter 1991). Também conhecida como Pseudoraiva, a Doença de Aujeszky é considerada uma importante causa de perdas econômicas em rebanhos de suínos, estando difundida em todo o mundo, inclusive no Brasil (Cândido et al 2000, Piatti et al 2001. O VDA compartilha características comuns a outros membros da família Herpesviridae.…”