Serum neutralization as a differential serological test for classical swine fever virus and other pestivirus infections

Abstract

Serum neutralization tests (SN) were performed against classical swine fever virus (CSFV), bovine viral diarrhea virus (BVDV) and border disease virus (BDV) on samples of swine serum collected for screening of antibodies to CSFV, in order to determine the SN value as a differential serological test. Ninety-nine sera out of a sample of 16,664 were positive for antibodies to pestiviruses in an ELISA test which did not distinguish antibodies to different pestiviruses. When submitted to SN, 81 sera were positive for CSFV antibodies only. In 17 sera, crossreactive antibodies to either CSFV, BVDV or BDV were detected. In most of these sera (13 out of 17) the differences between SN titres against the three viruses were not sufficient to estimate which was the most likely antibody-inducing virus. It was concluded that, for the SN to be useful in such differentiation, it is essential to examine a sample which must include a representative number of sera from the same farm where suspect animals were detected. When isolated serum samples are examined, such as those obtained with the sampling strategy adopted here, the SN may give rise to inconclusive results.

A infecção pelo vírus da diarréia viral bovina (BVDV) no Brasil: histórico, situação atual e perspectivas

“…O teste utiliza antígenos recombinantes produzidos em baculovírus e demonstrou sensibilidade de 97,6 % e especificidade de 99,4 % quando comparado com a SN. Paredes et al (1999) avaliaram a capacidade de um teste de soroneutralização de diferenciar a resposta sorológica entre os diferentes pestivírus. Kreutz et al (2000) produziram dois anticorpos monoclonais (mAbs) contra antígenos de um isolado brasileiro de BVDV-1.…”