Development of DNA probe for detection of Aujeszky's disease virus

Abstract

A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.

Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil

“…In the male, viral isolation was also unsuccessful, and no anatomopathological lesions consistent with the disease were observed. The latent infection of SuHV-1 confirmed by PCR in serologically nonreactive pigs was previously reported (Cândido et al, 1999;Ruiz-Fons et al, 2007; Mü ller et al, 2011). .…”