Caracterização dos tipos celulares presentes na hemolinfa de larvas e ninfas de Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) em diferentes estados nutricionais

Abstract

ABSTRACT. ClIARACTER IZATION OF TIIE CELULAR TYPES PRESENT lN TII E IIAEMOLYMPII OF LARVAE AND NIMPHS OF RHlPlCEI'IIALUS SAN(;UINEUS(LATREILLE) (IXODOIDEA, Ixo-DIDAE) lN DIFFERENT NUTRITIONAL STAGES. With lhe purpose of characterize morphologically lhe hemocytes of larvae and nymphs 01' Rhipicephalus sanguineus on ditTerent nutritional phases, it was obtained sal1lples ofhaemolYl1lph were obtained by sectioning the forel egs and collecting lhe drop there formed. A fter dried, the samples were fixed by methanol and stained by Giel1lsa. Microscopical observation resulled in lhe characlerization of five basic cellular lypes: prohemocyles, plasmatocytes, granulocytes, spherulocytes and oenocytoids. Moreover, undefined cell types, whose morphological patterns didn 't have correlation with the characleristics cited for hemocytes, were found in low frequency. The change in the relative cOl1lposition of the haemo-I imph was characterized by decrease of the number of granulocytes and greater variabilily of the cell types present in the hel1lolyl1lph as the tick evolved. This fact l11ay be linked to the alterations that lhese ce lls sutTer aI ong the development of the tick.

Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes

“…Spherulocytes are less abundant cells in the hemolymph, however, in R. sanguineus they appear to be more abundant (Carneiro and Daemon, 1997; Feitosa et al, 2015). In the present study, these cells present spherules in the cytoplasm and less organelles corroborating with other studies with insects (Brayner et al, 2005) and tick (Sonenshine and Hynes, 2008).…”

“…Studies about R. microplus cellular morphology and ultrastructure are scarce; for this reason, hemocytes identification through light and transmission microscopy was based on other tick species and other arthropods (Carneiro and Daemon, 1997; Borovicková and Hypsa, 2005; Habeeb and El-Hag, 2008; Feitosa et al, 2015, 2018). Silva et al (2006) studies with light microscopy reported six hemocytes types in R. microplus hemolymph described as prohemocytes, granulocytes, plasmatocytes, spherulocytes, adipohemocytes, and oenocytoids.…”

“…They are non-phagocytic cells but may be involved in the encapsulation process (Eggenberger et al, 1990). At light microscopy these cells show homogeneous cytoplasm or small refractory granulations (Carneiro and Daemon, 1997). Transmission microscopy revels few organelles, but some mitochondria and electron luscent vesicles may be present (Giulianini et al, 2003).…”

Entomopathogenic fungi against South American tick species

“…Many studies on the tick immune system are limited in identifying and quantifying hemocytes, or in characterizing antimicrobial peptides (Sonenshine 1991;Carneiro and Daemon 1996;Pereira et al 2001;Silva and Bittencourt 2006). The expression of four forms of defensin was observed in the intestine, fat body and reproductive tract of Ornithodorus moubata (Nakajima et al 2001(Nakajima et al , 2002.…”