The research retrieves and presents the main ways of bridging the universe of cultural heritage conservation with the trends of sustainability and, in particular, discusses the role of microclimate diagnostics of areas used for museological functions in the definition of preventive conservation strategies based on the principles of such movement. This analysis is articulated with case studies in which the dynamic behaviour of physicalenvironmental parameters (temperature and humidity) collected during a one-year time interval in internal areas of three locations : one museum storage area at Museu Paulista of the University of São Paulo (in São Paulo), an exhibition room at Museu Casa da Xilogravura (in Campos do Jordão) and one of the storage areas of the General Archive of the University of São Paulo (in São Paulo), in the light of external climatic variations.In particular, in the case of the area at Museu Paulista, secluded spaces used to store collections were also investigated in order to understand how their use constitutes a mitigating measure to minimize harmful effects associated with microclimate factors. The results and their interpretation allow corroborating the importance of diagnostics of conservation areas as a planning tool for preventive actions alternative to traditional approaches based on mechanized and automated environmental control. Moreover, they enable envisioning passive strategies that value existing potentials, minimize individual vulnerabilities and concretely contribute to the articulation with a sustainable world.