Armazenamento do grão de pólen de cebola (Allium cepa L.)

Gomes, Patrícia Rocha; Raseira, M. do C. B.; Baudet, Leopoldo; Peske, Silmar Teichert

doi:10.1590/s0101-31222003000100003

Cited by 11 publications

(4 citation statements)

References 6 publications

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“…The observed pollen in vitro germination rates of eggplant pollen grain, using the current technique, were low comparing to some studies in vitro with other species. For instance, Gomes et al (2003) observed 49.8% germination in onion pollen grains, while Dutra et al (2000) obtained 82.0% germination in pepper pollen and Franzon et al (2005) 79.7% in beans.…”

Section: In Vitro Germination Testmentioning

confidence: 97%

“…The pollen grain viability, a measure of male fertility, may be determined by different techniques (Dafni 1992, Kearns andInouye 1993). These can be grouped into direct methods, such as the induction of in vitro (Dutra et al 2000, Gomes et al 2003, Pio et al 2007) and in vivo (Oliveira et al 2001) germination or indirect methods based on cytological parameters such as color (Shivanna and Johri 1985, Dafni 1992, Shivanna and Rangaswamy 1992, Kearns and Inouye 1993.…”

Section: Introductionmentioning

confidence: 99%

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Viability of eggplant pollen

França¹,

Nascimento²,

Carmona³

et al. 2009

CBAB

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This study aimed to test methodologies to evaluate the pollen viability of eggplant. The pollen germination was tested in vitro, incubating the pollen during 0.5 and 16 hours in different sucrose concentrations (0; 2.5; 5.0; 7.5 and 10.0 g L -1 ). For the in vivo germination test, the pollen was placed during 4 and 24 hours in the flower stigma and after that stained with aniline blue and evaluated in an ultraviolet light microscope. The viability of the pollen grains was also evaluated in tetrazolium solutions (at the concentrations of 1%; 0.75% and 0.5%) and the staining was evaluated after 24 hours. The highest in vitro pollen germination was 10.8%, showing the necessity for a better calibration of the culture medium. The in vivo pollen germination was 66%, showing that this is an adequate methodology to estimate pollen viability in eggplant. The methodologies involving tetrazolium solution weren't efficient to estimate pollen viability evaluation, because of the deficient pollen staining.

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Section: In Vitro Germination Testmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Viability of eggplant pollen

França¹,

Nascimento²,

Carmona³

et al. 2009

CBAB

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“…The pollen grain storage is of special interest when natural flowering of the parents is difficult. In floriculture, this is especially interesting for the following reasons: the production of new cultivars is based on the use of hybridization techniques, with crosses between elite genotypes, followed by progenies evaluation and selection (Cardoso et al, 2016;Cardoso, 2017) and crosses between species with different flowering periods may result in F1 hybrids with natural flower production in more than one season per year, or even outside the flowering period of their parents (Gomes et al, 2003;Giovannini et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.)

2019

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The determination of viability and the preservation of pollen grains are useful tools for breeding of ornamentals, especially when the synchronization of flowering between species or cultivars does not occur naturally. Thus, the objective of this study was to characterize the flowering and to evaluate the preservation and maintenance of the viability of pollen grains in cultivars of amaryllis (Hippeastrum sp.) subjected to three storage temperatures. Anthers of five cultivars of amaryllis were collected from 12 to 24 hours after anthesis. One part of the anthers was immediately used for germination tests using in vitro germination in culture medium and the other part was stored at temperatures of 25, 8 and-20 °C. Pollen grains were germinated in culture medium at 0, 20, 75, 125, 165, 205 and 245 days after storage. All cultivars of amaryllis showed an accelerated loss of viability at 25 °C storage temperature. The 'Bull' cultivar showed higher recalcitrance to cold-storage, while 'Intokazie' and 'Pink Panther' cultivars showed higher longevity and higher percentage of germination along storage. The temperature of-20 °C resulted in the best responses for the maintenance of the integrity and preservation of the viability of pollen grains of different cultivars of amaryllis.

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“…Para possibilitar tais cruzamentos, há a necessidade de se dispor, prontamente, de pólen viável armazenado (sousa-Lang; Pinto, 1997). A conservação dos grãos de pólen é, portanto, de grande importância para a preservação da variabilidade genética além de facilitar o intercâmbio de germoplasma, contribuir significativamente na geração de variabilidade obtida através de cruzamentos artificiais e aumentar a eficiência dos programas de melhoramento (goMes et al, 2003).…”

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Análise Dos Grãos De Pólen De Diferentes Cultivares De Manona (Ricinus Communis L., Euphorbiaceae): Conservação E Viabilidade

et al. 2009

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RESUMO A análise da fertilidade dos grãos de pólen de Ricinus communis (mamona) é de grande importância para programas de melhoramento, permitindo o manejo e uso adequado das coleções existentes e a criação de cultivares interessantes na produção de biodiesel. Neste trabalho, optou-se por um processo preditivo baseado no método citológico de coloração dos grãos de pólen e no método de germinação in vitro dos grãos, após tratamentos de conservação a baixas temperaturas, para a verificação da viabilidade, importante na formação das sementes, alvo dos programas de melhoramento. Utilizou-se a técnica de coloração com carmim acético 2% na análise da viabilidade dos grãos de pólen nas cultivares IAC-80, Cafelista, AL-Preta e AL-Guarany 2002, enquanto que o método de germinação in vitro após tratamento de -196° C, -80° C e -18° C, por 15 e 30 dias, foi aplicado somente na cultivar IAC-80. A viabilidade polínica foi acima de 95% em IAC-80 e Cafelista de 88,48% em AL-Preta e de 86,46% em e AL-Guarany 2002. Estas cultivares apresentaram também alto percentual de viabilidade polínica na antese, sendo possível a utilização de todas as cultivares analisadas na indução de fertilização em programas de melhoramento. Os resultados obtidos com a germinação in vitro dos grãos de IAC-80, após a criopreservação, indicaram que o percentual médio da viabilidade polínica in vivo não foi influenciado pelo período de armazenamento. Observaram-se diferenças entre os tratamentos a baixas temperaturas, porém o percentual de viabilidade dos mesmos foi baixo, sugerindo necessidade de adequação na técnica de avaliação.

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Armazenamento do grão de pólen de cebola (Allium cepa L.)

Cited by 11 publications

References 6 publications

Viability of eggplant pollen

Viability of eggplant pollen

Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.)

Análise Dos Grãos De Pólen De Diferentes Cultivares De Manona (Ricinus Communis L., Euphorbiaceae): Conservação E Viabilidade

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