2012
DOI: 10.1590/s0101-20612012005000118
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Abstract: Application of Doehlert experimental design in the optimization of experimental variables for the Pseudozyma sp. (CCMB 306) and Pseudozyma sp. (CCMB 300) cell lysis Aplicação do modelo Doehlert na otimização das variáveis experimentais para a lise de Pseudozyma sp. (CCMB 306) e Pseudozyma sp. (CCMB 300)Amanda Reges de SENA 1,3 , Gildomar Lima VALASQUES JÚNIOR 1,2 , Ingara Keisle São Paulo BARRETTO 1 , Sandra Aparecida ASSIS 1 * IntroductionThe cell wall of yeast is basically made of glucan and mannan-protein. … Show more

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Cited by 12 publications
(8 citation statements)
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“…For optimizing the surface properties of APPJ‐treated PDMS in order to maximize the surface functionalization conferred to the surface by the treatment, the two‐factor Doehlert experimental plan design using the torch speed and the nozzle‐PDMS distance was performed, studying both parameters at five and three levels, respectively; one replicate experimental run similar to that in the center of the domain is also studied. The limits of the domain were set between 10 and 50 m min −1 of torch speed and between 5 and 9 mm of nozzle‐PDMS distance, according to the results shown in the previous section.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…For optimizing the surface properties of APPJ‐treated PDMS in order to maximize the surface functionalization conferred to the surface by the treatment, the two‐factor Doehlert experimental plan design using the torch speed and the nozzle‐PDMS distance was performed, studying both parameters at five and three levels, respectively; one replicate experimental run similar to that in the center of the domain is also studied. The limits of the domain were set between 10 and 50 m min −1 of torch speed and between 5 and 9 mm of nozzle‐PDMS distance, according to the results shown in the previous section.…”
Section: Resultsmentioning
confidence: 99%
“…First, a screening design based on a Plackett–Burman matrix (seven factors, eight experiments) was carried out in order to select the most influent plasma parameters on the surface activation of PDMS. Then, a response surface methodology (RSM) using a Doehlert Design (two factors, eight experiments) was used to optimize the wettability of PDMS substrates. The significance of each coefficient was determined by calculating the p ‐value and the model validity was checked by the analysis of variance (ANOVA).…”
Section: Methodsmentioning
confidence: 99%
“…Screening design based on Plackett-Burman matrix was selected and Doehlert experimental plan was chosen because of its spherical domain with uniformity in space filling, its ability to extend the results obtained for two-factors to three factors, its ability to explore the whole domain and its potential to reuse the experiment when the boundaries have not been well chosen initially [23].…”
Section: Statistical Design Of Experimentsmentioning
confidence: 99%
“…(14). In addition, the value of the adjusted coefficient of determination ( R 2 Adj = 0.992) is also very high to advocate for a high significance of the model [26].…”
Section: (13)mentioning
confidence: 99%