2012
DOI: 10.1590/s0101-20612012005000030
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: This work investigated the fructooligosaccharides (FOS) synthesis by immobilized inulinase obtained from Kluyveromyces marxianus NRRL Y-7571 in aqueous and aqueous-organic systems using sucrose as substrate. The sequential strategy of experimental design was used to optimize the FOS conversion in both systems. For the aqueous-organic system, a 2(6-2) fractional design was carried out to evaluate the effects of temperature, sucrose concentration, pH, aqueous/organic ratio, enzyme activity, and polyethylene glyc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2014
2014
2022
2022

Publication Types

Select...
3
2

Relationship

0
5

Authors

Journals

citations
Cited by 5 publications
(2 citation statements)
references
References 16 publications
0
2
0
Order By: Relevance
“…Results obtained high purity FOS (91.4%) with FOS titer, productivity, and yield of 717.3 g/L, 358.6 g/L/h and 0.912 gFOS/g inulin, respectively ( Mao et al, 2019 ). Risso et al (2012) investigated the FOS production by carrier-supported inulinase from K. marxianus NRRL Y-7571 in aqueous and aqueous-organic systems using sucrose as a substrate. In the case of an aqueous–organic system, the utmost yield of FOS (18.2 ± S0.9 wt%) was achieved at optimal condition of pH 5.0, 40°C, aqueous/organic ratio (25/75 wt%), enzyme activity (4 U/ml), and sucrose concentration of 60% (w/w), whereas the highest yield of FOS was achieved to be 14.6 ± 0.9 wt% for the aqueous system.…”
Section: Applications In Biotechnological Productsmentioning
confidence: 99%
“…Results obtained high purity FOS (91.4%) with FOS titer, productivity, and yield of 717.3 g/L, 358.6 g/L/h and 0.912 gFOS/g inulin, respectively ( Mao et al, 2019 ). Risso et al (2012) investigated the FOS production by carrier-supported inulinase from K. marxianus NRRL Y-7571 in aqueous and aqueous-organic systems using sucrose as a substrate. In the case of an aqueous–organic system, the utmost yield of FOS (18.2 ± S0.9 wt%) was achieved at optimal condition of pH 5.0, 40°C, aqueous/organic ratio (25/75 wt%), enzyme activity (4 U/ml), and sucrose concentration of 60% (w/w), whereas the highest yield of FOS was achieved to be 14.6 ± 0.9 wt% for the aqueous system.…”
Section: Applications In Biotechnological Productsmentioning
confidence: 99%
“…Although some bacteria such as Xanthomonas and Streptomyces are reported as good inulinase producers [19][20][21], fungi are among the most used, especially the Aspergillus genus [17,[22][23][24][25][26]. Among the yeasts, good inulinase productions are reported by the genera Pichia [11,27] and Saccharomyces [28], but it is the yeasts of the Kluyveromyces genus, especially K. marxianus, that are the most studied and promising [18,[29][30][31][32] because of their high production, rapid growth and GRAS (Generally Recognized As Safe) classification [33]. Thus the objective of the present study was to verify the potential of yacon flour as carbon source to inulinase production by K. marxianus NRRL Y-7571 using a Central Composite Design (CCD) to carry out the experiments and Response Surface Methodology (RSM) to analyze the results.…”
Section: Introductionmentioning
confidence: 99%