Validação intralaboratorial de método quantitativo para determinação múltipla de resíduos de avermectinas em leite bovino por cromatografia líquida de alta eficiência com detecção de fluorescência

Souza, Scheilla Vitorino Carvalho de; Lima, Josefa Abucater; Teodoro, João C.; Junqueira, Roberto Gonçalves

doi:10.1590/s0101-20612007000400024

Cited by 19 publications

(11 citation statements)

References 14 publications

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“…Prod. Anim., Salvador, v.20, 1 -12, e0112019, In previous studies was observed variation in the recovery residues rates in bovine milk, with rates ranging from 68% to 85% for albendazole residues (Ruyck et al, 2000), 77.0 and 97% (Moreno et al, 2005) and 80.5% and 101.3% (Xi et al, 2010).For ivermectin residues were obtained mean recovery rates of 78.8% (Alvinerie et al, 1994), ≥ 60.0% (Turnipseed et al, 2005) and ≥84.0% (Souza et al , 2007). Analyzing these previous studies together with the present results is plausible to admit that the recovery rate of albendazole residues and macrocyclic lactones may be influenced by the matrix and/or the method used.…”

Section: Rev Bras Saúdementioning

confidence: 94%

“…In fortified samples of bovine milk were observed albendazole retention time of 5.86 minutes using HPLC/MS (Whelan et al, 2010) and ivermectin retention time of 6.9 and 11.2 minutes using HPLC-FL (Flajs et al , 2005;Souza et al, 2007). Even though these times were greater than those found in the present study, it is emphasized that the methodologies used were different, including the fact that the vermifuges were added to milk samples from untreated animals.…”

Section: Rev Bras Saúdementioning

confidence: 99%

“…Although the milk production destined to consumption should avoid to the maximum the presence of pharmacological residues that cause risks to human health (Anvisa, 2006), thedrugs administration aimed to endoparasites control in dairy herds is a frequent practice in Brazil. Benzimidazoles residues (Brandon et al, 2002) and ivermectin residues (Turnipseed et al, 2005;Souza et al, 2007) were detected in bovine milk destined tohuman consumption. Due to the low cost and the possibility of use in lactating females, benzimidazoles have been the most used antiparasitic drugs in the last decades (Dayan, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Ivermectin and albendazole withdrawal period in goat milk

Santos

Cruz

Santos

et al. 2019

Rev. bras. saúde prod. anim.

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SUMMARY Theaimthis study was to determine the excretion profile of albendazole and ivermectin residues in milk from goats submitted to antiparasitic treatment. Twenty-four Brazilianmongrel lactating and pluriparous goats, maintained extensively on native pasture were orally treatedwith albendazole or ivermectin. Milk samples were collected before and after vermifuges application, in the days 0, 2, 3 e 4 to albendazole and 0, 3, 7, 14, 21, 28, 35 and 42 to ivermectin. The vermifuges residues were detected by high performance liquid cromatography with ultravioletdetector. The amount of residues contained in themilk was decreasing in function of time. The mean daily rates of decrease of albendazole residues were 63.34%, 40.18 and 100.0%, from the 2ndto the 4thday, respectively; on the 3rdday after treatment, 50% of the samples showed concentrations ≥ 47.61 μg.mL–1, and on the 4thday, no sample had albendazole residue. The amount excreted of ivermectin was similar between the 3rdand 21stday when all samples presented values ≥ 51.90 μg.mL-1; on the 35th day, 50% of the samples showed values above of recommended levels, and on the 42nd day, no sample had detectable ivermectin residue. In conclusion, the milk of Brazilian mongrel goats treated orally with albendazole or ivermectin does not contain its respective residues in detectable amounts from the 4th and 42nddays, respectively, after antiparasitic treatment.

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Section: Rev Bras Saúdementioning

confidence: 94%

Section: Rev Bras Saúdementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ivermectin and albendazole withdrawal period in goat milk

Santos

Cruz

Santos

et al. 2019

Rev. bras. saúde prod. anim.

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“…Studies employing statistical tests for validation of the analysis methods in foods have been utilized to give credibility to the results. 95 Accuracy and precision of the method To determine accuracy of the method, beverage samples in triplicate (free of pesticides) were fortified with 100.0, 200.0 and 1000.0 μg L -1 of the pesticides. The recovery rates obtained by the proposed method varied from 93 to 97%, with coefficients of variation less than 5%.…”

Section: Limit Of Detection Limit Of Quantification and Linearitymentioning

confidence: 99%

“…Studies employing statistical tests for validation of the analysis methods in foods have been utilized to give credibility to the results. 95 Accuracy and precision of the method …”

mentioning

confidence: 99%

Determination of carbamates in beverages by liquid-liquid extraction with low temperature partitioning and liquid chromatography

Goulart¹,

Alves²,

Paula³

et al. 2012

J. Braz. Chem. Soc.

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Carbamatos são os pesticidas mais frequentemente encontrados em alimentos e bebidas de coloração escura em casos de intoxicação acidental ou intencional. Neste trabalho, extração líquido-líquido com partição em baixa temperatura (LLE-LTP) foi otimizada e validada para determinação dos carbamatos aldicarb, carbofuran e carbaril em bebidas de sucos de uva e leite achocolatado. Este método envolve a extração com acetonitrila, partição líquido-líquido em baixa temperatura e análise por cromatografia líquida de alta eficiência com detecção ultravioleta (HPLC-UV). O método é rápido, eficiente e de baixo custo, emprega pequenos volumes de solvente por amostra e não necessita de limpeza dos extratos. O método de extração foi seletivo e apresentou porcentagens de extração acima de 90%. As premissas relacionadas com os testes estatísticos de linearidade foram verificadas e confirmadas. O método de extração e análise foi validado com resultados satisfatórios e pode ser aplicado em análises de rotina e forenses.Carbamates are the pesticides most commonly found in dark colored foods and beverages in cases of accidental or intentional poisoning. In this work, the liquid-liquid extraction with low temperature partitioning (LLE-LTP) was optimized and validated for determination of the carbamates aldicarb, carbofuran and carbaryl in grape juice and chocolate milk beverages. This method involved extraction with acetonitrile, liquid-liquid partition at low temperature and the analysis by high performance liquid chromatography with ultraviolet detection (HPLC-UV). The method is rapid, efficient and of low-cost, employing small volumes of solvent per sample and requiring no cleanup of the extracts. The extraction methodology was selective and presented recovery percentages above 90%. The premises related to the statistical linearity tests were checked and confirmed. The method of extraction and analysis was validated with satisfactory results, and may be applied in forensic and routine analysis Keywords: carbamates, low temperature partitioning, high performance liquid chromatography, sample preparation, validation IntroductionCarbamate insecticides are widely used in gardening and agriculture due to their broad spectrum of activity and relatively short environmental persistence. However, they are potentially toxic to humans, particularly in the context of intentional self-poisoning. 1,2 Carbamates are found as commercialized products for agricultural practices and some compounds of this group, with their respective commercial names are: carbaryl (Sevin ® ), aldicarb (Temik ® ) and carbofuran (Furadan ® ). From these, aldicarb stands out as the main target on the list of controlled pesticides of the United States Environmental Protection Agency (EPA) due to its elevated toxicity to mammals. 3,4 The maximum residue level (MRL) of aldicarb 5,6 and carbofuran 6 in water is 10 and 7 μg L -1 , respectively. In Goulart et al. 1155 Vol. 23, No. 6, 2012 milk, the MRLs established for aldicarb, 7,8 Exposures may occur accidentally, with ...

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