Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK) kernel globulin

Ramos, Cíntia Maria Pinto; Bora, Pushkar Singh

doi:10.1590/s0101-20612004000100024

Cited by 8 publications

(5 citation statements)

References 19 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The solubility was decreasing with decreasing pH values, eventually plateauing at pH 2–3, where the solubility was only about 30 %. The pattern of protein solubility of the macroalgal biomass differs from those reported, for example, for nuts (Ramos and Bora 2004 ), whey (Mulcahy et al 2016 ) and fish (Undeland et al 2003 ), the curves of which were U-shaped with a clear dip in solubility around pH 4–6. It is, however, in accordance with other studies of marine algal proteins, with the same slope-shaped solubility curves.…”

Section: Resultscontrasting

confidence: 55%

pH-driven solubilization and isoelectric precipitation of proteins from the brown seaweed Saccharina latissima—effects of osmotic shock, water volume and temperature

Vilg

Undeland

2016

J Appl Phycol

View full text Add to dashboard Cite

In the light of the global search for novel and sustainable protein sources, macroalgal proteins are becoming an attractive target. To date, mainly red and green macroalgae have been investigated in this respect, whereas the brown species are less studied, possibly because of the lower content of protein. In a biorefinery context, however, the protein content of brown macroalgae can still be economically interesting due to fast growth and the possibility to co-extract other compounds, such as alginates. The aim of this study was to develop a simple, scalable pH shift-based protein isolation technique applicable on wet Saccharina latissima biomass. Factors investigated were extraction volume, temperature, protein solubilization pH, osmoshock pretreatment and protein precipitation pH. Maximum protein solubility was obtained at pH 12, where 34 % of the total protein content could be extracted with 5.56 volumes of extraction solution (20 volumes on dry weight (dw) basis). Osmoshocking significantly increased the yield, and 20, 40 and 60 volumes of water (dw basis) gave 45.1, 46.8 and 59.5 % yield, respectively. The temperature during osmoshocking did not significantly affect the extraction yield, and extended time (16 vs. 1 or 2 h) reduced protein yield. Precipitation of solubilized proteins was possible below pH 4; the highest precipitation yield, 34.5 %, was obtained at pH 2. After combined alkaline extraction and acid precipitation, 16.01 % of the Saccharina proteins were recovered, which can be considered acceptable in comparison to other studies on algae but leaves some room for improvement when compared to protein extraction from, for instance, soy.

show abstract

Section: Resultscontrasting

confidence: 55%

pH-driven solubilization and isoelectric precipitation of proteins from the brown seaweed Saccharina latissima—effects of osmotic shock, water volume and temperature

Vilg

Undeland

2016

J Appl Phycol

View full text Add to dashboard Cite

show abstract

“…The main technological functional properties of passion fruit albedo flour were evaluated based on the following analyses: water absorption capacity (WAC): determined by the methods described by Sosulski et al (1976) and Ramos & Bora (2004. Water Solubility Index (WSI): determined according to the methodology proposed by Anderson et al (1970) with adaptations.…”

Section: Technological Functional Propertiesmentioning

confidence: 99%

“…Water Solubility Index (WSI): determined according to the methodology proposed by Anderson et al (1970) with adaptations. Oil absorption capacity (OAC): determined by a combination of the methods described by Ramos & Bora (2004 and Gong et al (2016). Emulsifying capacity (EMC) and foam stability (FS): determined according to the methods described by Ramos & Bora (2004 and Coffmann & Garciaj (1977).…”

Section: Technological Functional Propertiesmentioning

confidence: 99%

“…Oil absorption capacity (OAC): determined by a combination of the methods described by Ramos & Bora (2004 and Gong et al (2016). Emulsifying capacity (EMC) and foam stability (FS): determined according to the methods described by Ramos & Bora (2004 and Coffmann & Garciaj (1977). Gelling property (GP): determination according to the methodology of Lawal & Adebowale (2005).…”

Section: Technological Functional Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive, technological-functional potential and morphological structures of passion fruit albedo (Passiflora edulis)

et al. 2022

View full text Add to dashboard Cite

Agroindustrial disposal generates tons of passion fruit albedo during pulp and juice processing. The reduction of this environmental pollution potential and its use involves research such as this one, which aims to determine the bioactive compounds in the passion fruit albedo (Passiflora edulis), its technological, functional potential and its morphological structures, aiming at food application. The methodologies applied followed internationally accepted and recommended guidelines. The results showed that the albedo flour obtained a yield of 9.76%, and from this, a semifine powder flour was prepared (75.8%), with 4.95% moisture, 40.72% reducing sugars, water activity of 0.37, pH of 5.29, total titratable acidity of 0.99 and total soluble solids of 1.5 °Brix. In addition, the presence of bioactive compounds such as polyphenols (18.88 mg AGE/100 g), flavonoids (13.51 mg/100 g), anthocyanins (1.74 mg/100 g) and chlorophyll b (69.65 mg/100 g) was evidenced, highlighting a considerable amount of vitamin C (377.36 mg/100 g) and pectin concentration (40.5%). In addition, the presence of bioactive compounds such as polyphenols (18.88 mg AGE/100 g), flavonoids (13.51 mg/100 g), anthocyanins (1.74 mg/100 g) and chlorophyll b (69.65 mg/100 g) was evidenced, highlighting a considerable amount of vitamin C (377.36 mg/100 g) and pectin concentration (40.5%).

show abstract

“…Comparing the theoretical and the observed value of the molecular weight (MW) and pI of each protein, we noticed that the majority (76%) was identified at the expected position on the gels (∆MW <20% and ∆pI <0.6, according to Dupont et al, 2005). Approximately, 30% of the 44 identified proteins were represented by more than one spot, suggesting the occurrence of post-translational modifications, such as acetylation, phosphorylation and glycosylation (Ramos and Bora, 2004;Mayr et al, 2005;Zhu et al, 2005;Löster and Kannicht, 2008).…”

Section: Resultsmentioning

confidence: 92%

Proteomic analysis of non-tumoral breast tissue

Costa¹,

Kaviski²,

Souza³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Breast cancer is a complex and heterogeneous disease. In spite of the advances made in recent decades, a better understanding of the intrinsic mechanisms of this disease is crucial. The development of new biomarkers is absolutely necessary to improve diagnosis and prognosis. Research using the proteomic approach has generated interesting results; however, the complexity of the mammary gland and of breast tumors remains a major limitation to the development of new markers. An initial step is to characterize non-tumoral human breast tissue. We present data from classical proteomic analysis based on 2-D electrophoresis and peptide mass fingerprinting identification, which were performed on six non-tumoral samples from patients with invasive ductal breast carcinomas. Forty-four different proteins from 70 spots were identified and classified according to their biological function. Cytoskeleton and associated proteins represent the largest class (30%) followed by the proteins with binding function (27%). Several of the proteins have been described in breast tumors, such as vimentin, endoplasmin, small heat shock beta-6, disulfide isomerase ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4) 2430-2442 (2011) Proteomics of mammary tissue 2431and some cell growth, and proliferation regulators, suggesting the importance of including data on the characterization of non-tumoral breast and to studies on differential expression in cancer tissue.

show abstract

Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK) kernel globulin

Cited by 8 publications

References 19 publications

pH-driven solubilization and isoelectric precipitation of proteins from the brown seaweed Saccharina latissima—effects of osmotic shock, water volume and temperature

pH-driven solubilization and isoelectric precipitation of proteins from the brown seaweed Saccharina latissima—effects of osmotic shock, water volume and temperature

Bioactive, technological-functional potential and morphological structures of passion fruit albedo (Passiflora edulis)

Proteomic analysis of non-tumoral breast tissue

Contact Info

Product

Resources

About