Liposomal photosensitizers: potential platforms for anticancer photodynamic therapy

Muehlmann, Luís Alexandre; Joanitti, Graziella Anselmo; Silva, Jaqueline R.; Longo, João Paulo Figueiró; Azevedo, Ricardo Bentes

doi:10.1590/s0100-879x2011007500091

Cited by 49 publications

(53 citation statements)

References 51 publications

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“…[ 3 ] However, porphyrin-based sensitizers have a number of drawbacks which hinder their clinical application, such as their instability to photo-bleaching, hydrophobic nature, poor penetration depth, and phototoxicity. Considerable research effort has been directed toward improving photosensitizer delivery through the use of nanocarriers including polymeric micelles, [ 4 ] liposomal, [ 5 ] dendrimers, [ 6 ] silica-based, Most importantly, NIR thermal and photoacoustic (PA) contrast properties of the PMCS allow real-time imaging-guided precise therapy. We believe that this study lays a fi rm foundation for the wider use of carbon nanoparticles containing porphyrin-like moieties in conformal phototherapy.…”

Section: Doi: 101002/adma201602197mentioning

confidence: 99%

Metal–Organic‐Framework‐Derived Mesoporous Carbon Nanospheres Containing Porphyrin‐Like Metal Centers for Conformal Phototherapy

et al. 2016

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Section: Doi: 101002/adma201602197mentioning

confidence: 99%

Metal–Organic‐Framework‐Derived Mesoporous Carbon Nanospheres Containing Porphyrin‐Like Metal Centers for Conformal Phototherapy

et al. 2016

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“…That was expected, since the specific delivery of drugs to the tumor with nanobased systems is mainly dependent on the altered physiological characteristics of cancerous tissues in vivo. 38,39 Malignant neoplastic tissues have abnormal and aberrant blood vessels that are much more permeable to NPs when compared to normal noncancerous blood vessels. 39 This phenomenon is classically referred to as the enhanced permeability and retention effect, and is one of the most important theories explaining the enhanced drug delivery to tumor tissues related to nanobased drug carriers.…”

Section: Photodynamic Activity Against Cells In Vitromentioning

confidence: 99%

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Muehlmann¹,

Chiyin²,

Longo³

et al. 2014

IJN

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Photodynamic therapy is generally considered to be safer than conventional anticancer therapies, and it is effective against different kinds of cancer. However, its clinical application has been significantly limited by the hydrophobicity of photosensitizers. In this work, a system composed of the hydrophobic photosensitizer aluminum–phthalocyanine chloride (AlPc) associated with water dispersible poly(methyl vinyl ether-co-maleic anhydride) nanoparticles is described. AlPc was associated with nanoparticles produced by a method of solvent displacement. This system was analyzed for its physicochemical characteristics, and for its photodynamic activity in vitro in cancerous (murine mammary carcinoma cell lineage 4T1, and human mammary adenocarcinoma cells MCF-7) and noncancerous (murine fibroblast cell lineage NIH/3T3, and human mammary epithelial cell lineage MCF-10A) cell lines. Cell viability and the elicited mechanisms of cell death were evaluated after the application of photodynamic therapy. This system showed improved photophysical and photochemical properties in aqueous media in comparison to the free photosensitizer, and it was effective against cancerous cells in vitro.

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“…11,12,[43][44] It may be noted that photosensitive drugs encapsulated in the nanoparticles can be broadly classified into two categories. First, improvement of the solubility and bioavailability of the PDT drugs, [45][46][47] and second, utilization PDT drugs for nanoparticle destabilization to achieve on demand release of entrapped cargo (such as a second drug). Uniquely designed photosensitive lipids that can be activated by appropriate light sources for on-demand drug delivery are well documented and have been studied for decades.…”

Section: Discussionmentioning

confidence: 99%

Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts

Sine¹,

Urban²,

Thayer³

et al. 2014

IJN

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We recently reported laser-triggered release of photosensitive compounds from liposomes containing dipalmitoylphosphatidylcholine (DPPC) and 1,2 bis(tricosa-10,12-diynoyl)- sn -glycero-3-phosphocholine (DC 8,9 PC). We hypothesized that the permeation of photoactivated compounds occurs through domains of enhanced fluidity in the liposome membrane and have thus called them “Pocket” liposomes. In this study we have encapsulated the red light activatable anticancer photodynamic therapy drug 2-(1-Hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) (Ex/Em410/670 nm) together with calcein (Ex/Em490/517 nm) as a marker for drug release in Pocket liposomes. A mole ratio of 7.6:1 lipid:HPPH was found to be optimal, with >80% of HPPH being included in the liposomes. Exposure of liposomes with a cw-diode 660 nm laser (90 mW, 0–5 minutes) resulted in calcein release only when HPPH was included in the liposomes. Further analysis of the quenching ratios of liposome-entrapped calcein in the laser treated samples indicated that the laser-triggered release occurred via the graded mechanism. In vitro studies with MDA-MB-231-LM2 breast cancer cell line showed significant cell killing upon treatment of cell-liposome suspensions with the laser. To assess in vivo efficacy, we implanted MDA-MB-231-LM2 cells containing the luciferase gene along the mammary fat pads on the ribcage of mice. For biodistribution experiments, trace amounts of a near infrared lipid probe DiR (Ex/Em745/840 nm) were included in the liposomes. Liposomes were injected intravenously and laser treatments (90 mW, 0.9 cm diameter, for an exposure duration ranging from 5–8 minutes) were done 4 hours postinjection (only one tumor per mouse was treated, keeping the second flank tumor as control). Calcein release occurred as indicated by an increase in calcein fluorescence from laser treated tumors only. The animals were observed for up to 15 days postinjection and tumor volume and luciferase expression was measured. A significant decrease in luciferase expression and reduction in tumor volume was observed only in laser treated animal groups injected with liposomes containing HPPH. Histopathological examination of tumor tissues indicated tumor necrosis resulting from laser treatment of the HPPH-encapsulated liposomes that were taken up into the tumor area.

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Liposomal photosensitizers: potential platforms for anticancer photodynamic therapy

Cited by 49 publications

References 51 publications

Metal–Organic‐Framework‐Derived Mesoporous Carbon Nanospheres Containing Porphyrin‐Like Metal Centers for Conformal Phototherapy

Metal–Organic‐Framework‐Derived Mesoporous Carbon Nanospheres Containing Porphyrin‐Like Metal Centers for Conformal Phototherapy

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts

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Liposomal photosensitizers: potential platforms for anticancer photodynamic therapy

Cited by 49 publications

References 51 publications

Metal–Organic‐Framework‐Derived Mesoporous Carbon Nanospheres Containing Porphyrin‐Like Metal Centers for Conformal Phototherapy

Metal–Organic‐Framework‐Derived Mesoporous Carbon Nanospheres Containing Porphyrin‐Like Metal Centers for Conformal Phototherapy

Aluminum&ndash;phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Photo activation of HPPH encapsulated in&nbsp;&ldquo;Pocket&rdquo; liposomes triggers multiple drug release and tumor cell killing in mouse breast&nbsp;cancer xenografts

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Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Photo activation of HPPH encapsulated in “Pocket” liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts