Construction of a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen and mouse 4-1BBL genes and its effect on dendritic cells

Wang, Xiaodong; Kuang, Youlin; Liu, Xiuheng; Chen, Zhiyuan; Zhu, Hengcheng; Chen, Hui; Jiang, Bo; Shen, Hao

doi:10.1590/s0100-879x2011007500002

Cited by 1 publication

(1 citation statement)

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“…Chen et al (10) successfully induced osteoblastic differentiation of BMSCs using recombinant adenovirus Ad-BMP2, which consistently produced BMP2, and the adenovirus did not cause a strong immune response in the body. Weng et al (11) constructed a recombinant adenovirus coexpressing truncated human prostate-specific membrane antigen and mouse 4-1BBL genes using the internal ribosome entry site (IRES) sequence. However, the expression of more than two genes within a single vector using conventional approaches has several limitations, such as imbalanced protein expression and large size of the transgene cassette.…”

Section: Introductionmentioning

confidence: 99%

Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

Liu

Guo

et al. 2013

Braz J Med Biol Res

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Bone morphogenetic protein 2 (BMP2) and basic fibroblast growth factor (bFGF) have been shown to exhibit a synergistic effect to promote bone repair and healing. In this study, we constructed a novel adenovirus with high coexpression of BMP2 and bFGF and evaluated its effect on osteogenic differentiation of goat bone marrow progenitor cells (BMPCs). Recombinant adenovirus Ad-BMP2-bFGF was constructed by using the T2A sequence. BMPCs were isolated from goats by density gradient centrifugation and adherent cell culture, and were then infected with Ad-BMP2-bFGF or Ad-BMP2. Expression of BMP2 and bFGF was detected by ELISA, and alkaline phosphatase (ALP) activity was detected by an ALP assay kit. In addition, von Kossa staining and immunocytochemical staining of collagen II were performed on BMPCs 21 days after infection. There was a high coexpression of BMP2 and bFGF in BMPCs infected with Ad-BMP2-bFGF. Twenty-one days after infection, ALP activity was significantly higher in BMPCs infected with Ad-BMP2-bFGF than in those infected with Ad-BMP2. Larger and more mineralized calcium nodules, as well as stronger collagen II staining, were observed in BMPCs infected with Ad-BMP2-bFGF than in those infected with Ad-BMP2. In summary, we developed a novel adenovirus vector Ad-BMP2-bFGF for simultaneous high coexpression of BMP2 and bFGF, which could induce BMPCs to differentiate efficiently into osteoblasts.

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Section: Introductionmentioning

confidence: 99%