The use of polymerase chain reaction for early diagnosis of tuberculosis in Mycobacterium tuberculosis culture

Chagas, Mariana; Silva, R M da; Bazzo, Maria Luiza; Santos, Jairo Ivo dos

doi:10.1590/s0100-879x2010007500031

Cited by 8 publications

(8 citation statements)

References 22 publications

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“…Several studies showed similar results to our study (21)(22)(23). However, some studies reported a lower sensitivity of about 20% to 50% (20,21,24).…”

Section: Discussionsupporting

confidence: 92%

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Comparison of the culture and PCR methods for diagnosis of Mycobacterium tuberculosis in different clinical specimens

Gholoobi

Meshkat

2011

Clinical Biochemistry

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“…Several studies showed similar results to our study (21)(22)(23). However, some studies reported a lower sensitivity of about 20% to 50% (20,21,24).…”

Section: Discussionsupporting

confidence: 92%

“…Another report showed a PCR sensitivity of 85.7% and a specificity of 60% for diagnosis of M. tuberculosis in culture (22). Overall, our data showed that the buffer method is more efficient for direct DNA isolation compared with that of the boiling method.…”

Section: Discussionsupporting

confidence: 46%

Comparison of the culture and PCR methods for diagnosis of Mycobacterium tuberculosis in different clinical specimens

Gholoobi

Meshkat

2011

Clinical Biochemistry

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“…Acid-fast staining and Mtb culture are classical Mtb diagnosis techniques. The acid-fast stain lacks sensitivity, and the culture requires several weeks for incubation [ 3 , 4 ]. The inherent limitations make it difficult for them to meet the requirement for early diagnostics [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of in-house real-time PCR assay for Mycobacterium tuberculosis: a systematic review and meta-analysis

et al. 2019

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Background In recent years, studies on the diagnostic accuracy of in-house real-time PCR (hRT-PCR) assay for the detection of Mycobacterium tuberculosis ( Mtb ) have been reported with unignorable discrepancies. To assess the overall accuracy of the hRT-PCR assay for Mtb diagnosis in different samples for individuals with active pulmonary and extra-pulmonary Mtb infection, a systematic review and meta-analysis were performed. Methods The PUBMED, EMBASE, Web of Science, and Cochrane databases were searched up to June 2017 for eligible studies that estimated diagnostic sensitivity and specificity with the hRT-PCR assay in respiratory and non-respiratory samples in pulmonary and extra-pulmonary Mtb infection patients, with Mtb culture as the reference standard. Bivariate random effect models were used to provide pooled estimation of diagnostic accuracy. Further, subgroup and meta-regression analyses were performed to explore sources of heterogeneity. The risk of bias was assessed by the QUADAS-2 tool. Results Of the 3589 candidate studies, 18 eligible studies met our inclusion criteria. Compared to Mtb culture data, the pooled sensitivity and specificity were 0.96 and 0.92, respectively. The diagnostic odds ratio (DOR) was 192.96 (95% CI 68.46, 543.90), and the area under the summary ROC curve (AUC) was 0.9791. There was significant heterogeneity in sensitivity and specificity among the enrolled studies ( p < 0.001). The studies with high-quality assessment and application of respiratory specimen were associated with better accuracy. Conclusions In low-income/high-burden settings, our results suggested that the hRT-PCR assay could be a useful test for the diagnosis of TB with high sensitivity and specificity. Electronic supplementary material The online version of this article (10.1186/s12879-019-4273-z) contains supplementary material, which is available to authorized users.

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“…Sputum smear microscopy shows low sensitivity, and culture is usually time consuming due to the slow growth rate of M. tuberculosis (Chagas et al . ; Shinu et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…Conventional diagnostic methods have inherent limitations (Kh echine and Drancourt 2011). Sputum smear microscopy shows low sensitivity, and culture is usually time consuming due to the slow growth rate of M. tuberculosis (Chagas et al 2010;Shinu et al 2011). This may lead to severe consequences, including false-negative diagnosis, which may increase the risk of M. tuberculosis transmission, TB incidence and resistance to drugs (Helb et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a IS6110-Taqman real-time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB

et al. 2013

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Aim: Evaluate the IS6110-Taqman system performance in sputum samples from patients with pulmonary tuberculosis from health services in northeastern Brazil as a diagnostic laboratory tool for pulmonary tuberculosis. Methods and Results: 165 sputum samples from respiratory symptomatic patients were evaluated in the IS6110-TaqMan assay: 66 patients with pulmonary tuberculosis and 99 without TB. When the IS6110-TaqMan assay was evaluated using culture and/or clinical response to the specific treatment as the gold standard, IS6110-TaqMan assay obtained a sensitivity of 87Á9% and specificity of 98%. The performance of IS6110-TaqMan assay was also evaluated with the sputum smear microscopy, resulting in a sensitivity of 79Á7% and specificity 94Á8%. Conclusions: The IS6110-TaqMan was rapid, sensitive and specific for the diagnosis of pulmonary TB. Significance and Impact of the Study: IS6110-TaqMan assay is a promising auxiliary tool for the diagnosis of pulmonary TB when used in conjunction with routine laboratory tests, clinical and epidemiological criteria of the patient, thus increasing the sensitivity and specificity of diagnosis.

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The use of polymerase chain reaction for early diagnosis of tuberculosis in Mycobacterium tuberculosis culture

Cited by 8 publications

References 22 publications

Comparison of the culture and PCR methods for diagnosis of Mycobacterium tuberculosis in different clinical specimens

Comparison of the culture and PCR methods for diagnosis of Mycobacterium tuberculosis in different clinical specimens

Diagnostic accuracy of in-house real-time PCR assay for Mycobacterium tuberculosis: a systematic review and meta-analysis

Evaluation of a IS6110-Taqman real-time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB

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