Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats

Gonçalves, Reggiani Vilela; Mezêncio, J. M. S.; Benevides, Gustavo Pereira; Matta, Sérgio Luís Pinto da; Neves, Clóvis Andrade; Sarandy, Mariáurea Matias; Vilela, E. F.

doi:10.1590/s0100-879x2010007500022

Cited by 27 publications

(34 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Positive effects of gallium-aluminium-arsenide (GaAlAs)-laser, gallium-arsenide (GaAs) laser, and Dersani (linoleic acid) healing ointment on skin wounds in Wistar rats were determined in a study of Gonçalves et al [30]. After irradiation, lesions were analyzed and the tissues were studied by electron microscopy, histology, and immunohistochemistry.…”

Section: Resultsmentioning

confidence: 99%

Low Level Laser Therapy for the Treatment of Diabetic Foot Ulcers: A Critical Survey

Beckmann

Meyer-Hamme

Schröder

2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Diabetic foot ulcers as one of the most common complications of diabetes mellitus are defined as nonhealing or long-lasting chronic skin ulcers in diabetic patients. Multidisciplinary care for the diabetic foot is common, but treatment results are often unsatisfactory. Low level laser therapy (LLLT) on wound areas as well as on acupuncture points, as a noninvasive, pain-free method with minor side effects, has been considered as a possible treatment option for the diabetic foot syndrome. A systematic literature review identified 1764 articles on this topic. Finally, we adopted 22 eligible references; 8 of them were cell studies, 6 were animal studies, and 8 were clinical trials. Cell studies and animal studies gave evidence of cellular migration, viability, and proliferation of fibroblast cells, quicker reepithelization and reformed connective tissue, enhancement of microcirculation, and anti-inflammatory effects by inhibition of prostaglandine, interleukin, and cytokine as well as direct antibacterial effects by induction of reactive oxygen species (ROS). The transferral of these data into clinical medicine is under debate. The majority of clinical studies show a potential benefit of LLLT in wound healing of diabetic ulcers. But there are a lot of aspects in these studies limiting final evidence about the actual output of this kind of treatment method. In summary, all studies give enough evidence to continue research on laser therapy for diabetic ulcers, but clinical trials using human models do not provide sufficient evidence to establish the usefulness of LLLT as an effective tool in wound care regimes at present. Further well designed research trials are required to determine the true value of LLLT in routine wound care.

show abstract

Section: Resultsmentioning

confidence: 99%

Low Level Laser Therapy for the Treatment of Diabetic Foot Ulcers: A Critical Survey

Beckmann

Meyer-Hamme

Schröder

2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…; Gonçalves et al . ,b). The results of this study showed that both groups that received laser irradiation had a higher total collagen content at all time points analysed.…”

Section: Discussionmentioning

confidence: 99%

“…Macrophages are important cells responsible for releasing growth factors that stimulate proliferation, differentiation and synthesis of extracellular matrix components (Shields & O'Kane ; Reddy ; Gonçalves et al . ). In in vitro experimental models examination of a wide range of wavelengths showed that wavelengths between 524 nm and 904 nm were related to decreased time of wound healing by stimulating fibroblast and keratinocyte differentiation, collagen production and skin neovascularization (Pogrel et al .…”

mentioning

confidence: 97%

The energy density of laser light differentially modulates the skin morphological reorganization in a murine model of healing by secondary intention

Novaes

Gonçalves

Cupertino

et al. 2013

Int J Experimental Path

View full text Add to dashboard Cite

This study investigates the influence of gallium-arsenide (GaAs) laser photobiostimulation applied with different energy densities on skin wound healing by secondary intention in rats. Three circular wounds, 10 mm in diameter, were made on the dorsolateral region of 21 Wistar rats weighting 282.12 ± 36.08 g. The animals were equally randomized into three groups: Group SAL, saline solution 0.9%; Group L3, laser GaAs 3 J/cm(2); Group L30, laser GaAs 30 J/cm(2). Analyses of cells, blood vessels, collagen and elastic fibres, glycosaminoglycans and wound contraction were performed on the scar tissue from different wounds every 7 days for 21 days. On day 7, 14 and 21, L3 and L30 showed higher collagen and glycosaminoglycan levels compared to SAL (P < 0.05). At day 21, elastic fibres were predominant in L3 and L30 compared to SAL (P < 0.05). Type-III collagen fibres were predominant at day 7 in both groups. There was gradual reduction in these fibres and accumulation of type-I collagen over time, especially in L3 and L30 compared with SAL. Elevated density of blood vessels was seen in L30 on days 7 and 14 compared to the other groups (P < 0.05). On these same days, there was higher tissue cellularity in L3 compared with SAL (P < 0.05). The progression of wound closure during all time points investigated was higher in the L30 group (P < 0.05). Both energy densities investigated increased the tissue cellularity, vascular density, collagen and elastic fibres, and glycosaminoglycan synthesis, with the greater benefits for wound closure being found at the density of 30 J/cm(2).

show abstract

“…Rizzi et al [24] described better oxidative metabolism with six than 10 laser applications at 20 J/cm 2 in intact masseter muscle in mice. Gonçalves et al [34] observed an increase of the collagen with the GaAlAs laser (4 J/cm 2 and 30 J/cm 2 ), but no changes with the 60 J/cm 2 on rat skin injury. So, these facts corroborate reports that the lower power laser has photobiomodulator potential [10, 34, 35].…”

Section: Discussionmentioning

confidence: 99%

“…Gonçalves et al [34] observed an increase of the collagen with the GaAlAs laser (4 J/cm 2 and 30 J/cm 2 ), but no changes with the 60 J/cm 2 on rat skin injury. So, these facts corroborate reports that the lower power laser has photobiomodulator potential [10, 34, 35]. On the other hand, higher energy densities cause morphological changes, reduced mitochondrial activity, and risks to DNA [10, 36] and can also induce apoptosis [37].…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure and Light Microscope Analysis of Intact Skin after a Varying Number of Low Level Laser Irradiations in Mice

Iyomasa

Leão

Rizzi

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Low level laser therapy (LLLT) has been used to relieve pain, inflammation, and wound healing processes. Thus, the skin is overexposed to laser and this effect is not completely understood. This study analyzed the effects of the number of laser applications (three, six, and 10) on the intact skin of the masseteric region in mice of strain HRS/J. The animals (n = 30) were equally divided into control (0 J/cm2) and irradiated (20 J/cm2), and each of these groups was further equally divided according to the number of laser applications (three, six, and 10) and underwent LLLT on alternate days. Samples were analyzed by light microscopy and transmission electron microscope (TEM). The animals receiving applications exhibited open channels more dilated between the keratinocytes and photobiomodulation effect on endothelial cells and fibroblasts by TEM. Under the light microscope after 10 laser applications, the type I collagen decreased (P < 0.05) compared to the three and six applications. Under these experimental conditions, all numbers of applications provided photobiomodulatory effect on the epidermis and dermis, without damage. More studies are needed to standardize the energy density and number of applications recommended for laser therapy to have a better cost-benefit ratio associated with treatment.

show abstract

Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats

Cited by 27 publications

References 23 publications

Low Level Laser Therapy for the Treatment of Diabetic Foot Ulcers: A Critical Survey

Low Level Laser Therapy for the Treatment of Diabetic Foot Ulcers: A Critical Survey

The energy density of laser light differentially modulates the skin morphological reorganization in a murine model of healing by secondary intention

Ultrastructure and Light Microscope Analysis of Intact Skin after a Varying Number of Low Level Laser Irradiations in Mice

Contact Info

Product

Resources

About