Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains

Médici, Kerlei Cristina; Barry, Aline Fernandes; Alfieri, Alice Fernandes; Alfieri, Amauri Alcindo

doi:10.1590/s0100-879x2010000100003

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…Although group B rotavirus has already been described in rats [9] and pigs in Brazil [20], the submission of the NSP5 gene fragments generated in this study to BLAST/n at http://www.ncbi.nlm.nih.gov/BLAST confirmed group A rotavirus identity relatedness. A stretch of 259 nucleotides of NSP5 encoding gene residues, related to the N-terminal portion of the protein [16], was used to build a phylogenetic tree, on which the genotype segregation (H1–H11) was maintained [17], as shown in Figure 1.…”

Section: Discussionsupporting

confidence: 58%

Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil

Tonietti

Hora

Silva

et al. 2013

The Scientific World Journal

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This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine (n = 21) and rat (n = 6) fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results point toward a cross-species transmission between rats and pigs on the surveyed farm and represent the first detection of rotavirus in Rattus norvegicus in Brazil.

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Section: Discussionsupporting

confidence: 58%

Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil

Tonietti

Hora

Silva

et al. 2013

The Scientific World Journal

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“…These recent strains differed genetically from the Chinese strain [53,55], suggesting that diverse RVB strains are circulating in humans. Limited evidence for the zoonotic potential of some porcine RVB strains was provided by Medici and colleagues demonstrated a high nucleotide identity between the NSP2 gene sequences of human and porcine strains [198]. …”

Section: Zoonotic Potential Of Porcine Rv Strainsmentioning

confidence: 99%

Porcine Rotaviruses: Epidemiology, Immune Responses and Control Strategies

Vlasova

Amimo

Saif

2017

Viruses

180

197

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Rotaviruses (RVs) are a major cause of acute viral gastroenteritis in young animals and children worldwide. Immunocompetent adults of different species become resistant to clinical disease due to post-infection immunity, immune system maturation and gut physiological changes. Of the 9 RV genogroups (A–I), RV A, B, and C (RVA, RVB, and RVC, respectively) are associated with diarrhea in piglets. Although discovered decades ago, porcine genogroup E RVs (RVE) are uncommon and their pathogenesis is not studied well. The presence of porcine RV H (RVH), a newly defined distinct genogroup, was recently confirmed in diarrheic pigs in Japan, Brazil, and the US. The complex epidemiology, pathogenicity and high genetic diversity of porcine RVAs are widely recognized and well-studied. More recent data show a significant genetic diversity based on the VP7 gene analysis of RVB and C strains in pigs. In this review, we will summarize previous and recent research to provide insights on historic and current prevalence and genetic diversity of porcine RVs in different geographic regions and production systems. We will also provide a brief overview of immune responses to porcine RVs, available control strategies and zoonotic potential of different RV genotypes. An improved understanding of the above parameters may lead to the development of more optimal strategies to manage RV diarrheal disease in swine and humans.

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“…Porcine group A rotavirus (PoRVA) infections are most frequently identified in episodes of diarrhea in piglets worldwide, including in Brazil (Linares et al 2009, Halaihel et al 2010, Médici et al 2011, Lorenzetti et al 2011; however, the involvement of porcine rotavirus groups B and C (PoRVB and PoRVC) in weaning and post-weaning piglets with diarrhea has also been reported mainly in transversal epidemiological studies (Magar et al 1991, Zlotowski et al 2008, Médici et al 2010a, 2010b, Médici et al 2011, Marthaler et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections

et al. 2014

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Porcine group A rotavirus (PoRVA) is a major cause of neonatal diarrhea in suckling and recently weaned piglets worldwide. The involvement of non-group A rotavirus in cases of neonatal diarrhea in piglets are sporadic. In Brazil there are no reports of the porcine rotavirus group C (PoRVC) as etiologic agent of the diarrhea outbreaks in piglets. The aim of this study was to describe the identification of rotavirus group C in single and in mixed infection with rotavirus groups A and B in three neonatal diarrhea outbreaks in suckling (≤21-day-old) piglets, with 70% to 80% and 20% to 25% of morbidity and lethality rates, respectively, in three pig herds located in the state of Santa Catarina, Brazil. The diagnosis of PoRV in the diarrheic fecal samples was performed using polyacrylamide gel electrophoresis (PAGE) to identify the presence of porcine rotavirus groups A, B (PoRVB), and C, and by RT-PCR (PoRVA and PoRVC) and semi-nested (SN)-PCR (PoRVB) to partially amplify the VP4 (VP8*)-VP7, NSP2, and VP6 genes of PoRVA, PoRVB, and PoRVC, respectively. One RT-PCR (PoRVA and PoRVC) and SN-PCR (PoRVB) product of each group of rotavirus of each diarrhea outbreak was submitted to nucleotide (nt) sequence analysis. Based on the PAGE technique, 4 (25%) and 1 (6.25%) of the 16 diarrheic fecal samples evaluated in the first outbreak presented PoRVA and PoRVC electropherotype, respectively, and 11 (68.75%) were negative. In the second outbreak, 3 (42.85%) of the 7 fecal samples evaluated presented PoRVA electropherotype, and in 3 (42.85%) and in 1 (14.3%) fecal samples were detected inconclusive and negative results, respectively. Three (30%) of the 10 fecal samples of the third outbreak presented PoRVC electropherotype; 5 (50%) and 2 (20%) samples showed negative and inconclusive results, respectively. Based on the RT-PCR and SN-PCR assays in the first neonatal diarrhea outbreak, PoRVC was detected in 13 (81.2%) of the 16 diarrheic fecal samples evaluated. PoRVC single infection was identified in 4 (25%) of these samples and mixed infections with PoRVA and PoRVB in 9 (56.2%) fecal samples. All of the seven diarrheic fecal samples evaluated from the second neonatal diarrhea outbreak were positive for PoRVC, whereas its mixed infection with other PoRV groups was detected in 4 (57.2%) samples. In the third outbreak, PoRVC in single infection was detected in all of the 10 diarrheic fecal samples analyzed. In the nt sequence analysis, the PoRVA strains of the first and second outbreaks demonstrated higher nt identity with G4P[6] and G9P [23] genotypes, respectively. The PoRVB strains (first and second outbreaks) and the PoRVC

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Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains

Cited by 9 publications

References 18 publications

Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil

Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil

Porcine Rotaviruses: Epidemiology, Immune Responses and Control Strategies

Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections

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