Role of 11β-hydroxysteroid dehydrogenase 2 renal activity in potassium homeostasis in rats with chronic renal failure

Yeyati, N; Altuna, María Eugenia; Damasco, M C; Laughlin, Myriam Alicia Mac

doi:10.1590/s0100-879x2009007500028

Cited by 1 publication

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“…In recent studies, a lot of parameters were calculated to represent the enzyme activity of 11 β -HSD, including measuring the AUC ratio of prednisone/prednisolone in plasma [ 22 – 24 ] and ratio of (THF+5 α -THF)/THE [ 15 , 25 , 26 ] (THB+5 α -THB)/THA [ 27 ] (THF: tetrahydrocortisol, 5 α -THF: 5alpha-tetrahydrocortisol, THE: tetrahydrocortisone, THB: tetrahydrocorticosterone, 5 α -THB: 5alpha-tetrahydrocorticosterone, and THA: 11-dehydro-tetrahydrocorticosterone). Furthermore, 11 β -HSD activities could also be detected by microsomal incubation with radiolabeled 3H-corticosterone [ 28 , 29 ] and 3H-cortisone [ 30 ] as substrate. The purpose of this study was to examine the effect of drug combination with Luteolin on metabolism of prednisone/prednisolone through 11 β -HSD in liver and kidney.…”

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confidence: 99%

Effect ofLuteolinon 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney

Tang

Deng

Shi

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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11Beta-hydroxysteroid dehydrogenase (11β-HSD) enzymes control the glucocorticoid (GC) signaling, which is essential in regulating homeostasis. Our previous study revealed that Eclipta prostrata (EP) affected the activity and expression of 11β-HSD enzymes which might improve the efficacy and reduce the adverse drug effects of glucocorticoid in patients undergoing combinational therapy. However, it is still unclear which composition of EP plays a major role and how it works. In this paper, we chose Luteolin which is one of the main ingredients of EP and evaluated its effect and metabolism in combination with prednisone. The effects of different concentrations of Luteolin extract on prednisone/prednisolone metabolism indicated the enzyme activity of 11β-HSD, so the production rate (pmol/min per mg protein) of metabolites was used to indicate enzyme activity. Furthermore, we explored the influence of Luteolin on gene and protein expressions of 11β-HSD I/II in rat liver and kidney tissue. Our results showed that oral administration of Luteolin significantly increased the gene and protein expressions of hepatic 11β-HSD I and renal 11β-HSD II, which may improve the efficacy and reduce the adverse drug effect of glucocorticoid in clinical application. A potential clinical value of Luteolin would also be indicated in combination therapy with prednisone for the treatment of nephrotic syndrome.

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