Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment

Turell, Lucía; Carballal, Sebastián; Botti, Horacio; Radí, Rafael; Álvarez, Beatriz

doi:10.1590/s0100-879x2009000400001

Cited by 86 publications

(64 citation statements)

References 56 publications

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“…In addition, albumin sequesters prooxidant molecules and redoxactive metals. Moreover, albumin has been proposed to have a thioredoxin-dependent lipid hydroperoxide reductase activity in vitro (13,24).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Occupational Exposure to Lead on the Non-Enzymatic Antioxidant System

Dobrakowski¹,

Zalejska–Fiolka²,

Wielkoszyński³

et al. 2014

Med Pr

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Background: The role of non-enzymatic antioxidants, such as uric acid, albumin, bilirubin, and α-tocopherol, in lead poisoning remains unclear. Therefore, the aim of the study was to explore the association between occupational exposure to lead and nonenzymatic antioxidant concentrations in serum and plasma. Material and Methods: The study population consisted of 278 healthy male employees of lead-zinc plants, with 129 workers classified as having low lead exposure (blood lead level -PbB = 20-39.9 μg/dl) and 149 workers classified as having high lead exposure (PbB = 40-59.8 μg/dl). The control group was composed of 73 healthy male administrative workers. No one from this group had blood lead level or zinc protoporphyrin (ZPP) level greater than normal levels, being 10 μg/dl and 2.5 μg/g of hemoglobin, respectively. In addition to the levels of PbB and ZPP, serum levels of uric acid (UA), albumin, thiol groups of albumin, and bilirubin were determined. The ferric reducing ability of plasma (FRAP) and the plasma level of α-tocopherol were also evaluated. Results: Lead exposure indices were significantly elevated in the examined subgroups as compared with the controls. Serum uric acid levels were significantly elevated in both subgroups, particularly in the group with high exposure. Serum bilirubin concentration was significantly elevated in the group with high exposure compared with the control group, while in the group with low exposure, it showed only a non-significant trend towards an increase. In contrast, ferric-reducing ability of plasma was not significantly greater in the examined subgroups as compared with the control group. Nevertheless, levels of albumin, thiol groups of albumin, and α-tocopherol levels were significantly decreased in the exposed subgroups compared with the control group. Conclusions: Occupational exposure to lead interferes with the blood non-enzymatic antioxidant system. Med Pr 2014;65(4): [443][444][445][446][447][448][449][450][451]

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Section: Discussionmentioning

confidence: 99%

The Effect of Occupational Exposure to Lead on the Non-Enzymatic Antioxidant System

Dobrakowski¹,

Zalejska–Fiolka²,

Wielkoszyński³

et al. 2014

Med Pr

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“…It is estimated that about 70% of the total free thiol content in plasma exists in HSA Cys34 [32]. This pool of thiol compounds in plasma gives rise to thiol exchange reactions leading to a number of disulfide bonds, and thus to formation of dimers acting as antioxidants by scavenging hydroxyl or other radicals through the reduced sulfhydryl group [33].…”

Section: Discussionmentioning

confidence: 99%

Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

Spanidis

Priftis

Stagos

et al. 2017

Preprint

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Abstract:The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress after an exhaustive exercise such as ultramarathon race. Thus, blood samples from 12 adult runners who underwent a 103 km mountain ultra-marathon race were collected pre-and 24, 48 and 72 h post race. HSA was partially purified using affinity chromatography and then was subjected to Western blot analysis for disulfide dimers determination, indicating oxidation. The results were correlated with those from a previous study in which the same samples were analyzed using different oxidative stress markers and a good correlation with protein carbonyls (PC) at all time points was observed. Moreover there was a significant correlation with static oxidation-reduction potential (sORP) at 24 h, and a negative correlation with capacity oxidation-reduction potential (cORP) at 24 and 48 h. In addition, an individual analysis of albumin dimers exhibited great interindividual differences. This inter-individual variability in the oxidation of HSA may suggest different interventions (e.g. through diet) in order to confront the effects on athletes' organism after a strenuous exercise. In conclusion, this study supported the importance of the assessment of albumin dimers as a predictive marker for exerciseinduced oxidative stress.

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“…In this case, the role of IS is add up to the fact that in a zone of oxidative stress (vascular proteins and glomerular tissues) is occurred intensive oxidation of a large group of biomolecules, including nucleic acids and lipids. However, due to their higher content in tissues, proteins are preferential targets [31]. Detailed consideration about the possible influence of oxidative stress on proteins and the role of oxidative transformations on the cardiovascular morbidity of CKD patients, in our opinion, lead to the conclusion that the most significant is an oxidative conversion of protein thiol groups (R-SH) to irreversible higher oxidation states, namely to sulfonic groups (R-SO 3 H functionality).…”

Section: A Possible Mechanism Of Aortic Calcification Induced By Ismentioning

confidence: 97%

Mechanisms of Oxidative Stress an d Vessels Sclerotic Transformation Initiated by Uremic Toxin Indoxyl Sulfate

Стрелко¹,

Gorlov²

2016

Eur Chem Tech J

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The microspherical carbonaceous adsorbents SCN and AST-120 for oral use prevent chronic disease progression, delay dialysis initiation, lessen atherosclerosis development in kidney and decreased of overall and cardiovascular mortality. This therapeutic effect is due to the binding of indole by sorbents in large intestine, which is a precursor of indoxyl sulfate (IS). It is considered that IS accelerates the progression of chronical kidney disease (CKD) by inducing a formation of reactive oxygen species (ROS) and promotes aortic calcification (mineralization). Molecular mechanisms of IS action is unknown. Using density functional theory calculations in the frames of B3LYP exchange and correlation functional (basis set 6-311G) and solvation accounting on the base of polarizable continuum model (PCM) we have studied some chemical transformations of IS and have shown a possibility of indoxyl sulfate and hydroperoxyl radicals formation through the reaction of IS with endogenous singlet oxygen. Due to the high activity indoxyl sulfate radicals initiate uncontrollable processes of oxidative stress (OS) in kidney and vascular tissues that promote a development of CKD. We also proposed a hypothesis, which can explain the role of OS in the accelerated development of sclerosis (vessels mineralization) in patients with renal diseases. In particular it was hypothesized and then supported by B3LYP/6-311G(d) + PCM calculations that sulfonic groups (products of deep oxidation of thiol groups in tissue proteins under OS, induced by IS) can selectively bind of Ca 2+ ions and, consequently, forming RSO 3 Ca + groups which can fix НРО 4 2ˉand CO 3 2ˉ anions. The products of anions fixation can then bind of Ca 2+ ions, etc. Notably, these processes are, probably, primary starting point in case of sclerotic vessel changes. The beginning of this starting mineralization process most likely is possible with proteins carboxyl groups forming under OS that also can bind Ca 2+ .

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Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment

Cited by 86 publications

References 56 publications

The Effect of Occupational Exposure to Lead on the Non-Enzymatic Antioxidant System

The Effect of Occupational Exposure to Lead on the Non-Enzymatic Antioxidant System

Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

Mechanisms of Oxidative Stress an d Vessels Sclerotic Transformation Initiated by Uremic Toxin Indoxyl Sulfate

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