An ultrasound and histomorphological analysis of experimental liver cirrhosis in rats

Dias, Juliana; Paredes, Bruno Diaz; Mesquita, Luiz Fernando; Carvalho, Adriana Bastos; Kozlowski, Eliene O.; Lessa, Andréia; Takiya, Christina Maeda; Resende, C.M.C.; Coelho, H.S.M.; Carvalho, Antônio Carlos Campos de; Rezende, Guilherme; Goldenberg, Regina Coeli dos Santos

doi:10.1590/s0100-879x2008001100008

Cited by 16 publications

(9 citation statements)

References 37 publications

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“…Animals (n = 40) were randomly divided into two groups: the Control group (n = 10) was fed with standard rat chow and water, and the Experimental group (n = 30) had chronic liver damage induced according to the protocol previously described by Dias et al [10], Quintanilha et al [16] and Carvalho et al [32]. Briefly, liver damage was induced with injections of a 20% solution of carbon tetrachloride (CCl 4 ) (1:5 in olive oil, dose of 0.05 mL/kg) intraperitoneally three times a week on alternate days combined with an alcoholic liquid diet in accordance with the AIN-93 guidelines [33] over 15 weeks.…”

Section: Methodsmentioning

confidence: 99%

Ultrasound imaging in an experimental model of fatty liver disease and cirrhosis in rats

et al. 2010

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BackgroundDomestic dogs and cats are very well known to develop chronic hepatic diseases, including hepatic lipidosis and cirrhosis. Ultrasonographic examination is extensively used to detect them. However, there are still few reports on the use of the ultrasound B-mode scan in correlation with histological findings to evaluate diffuse hepatic changes in rodents, which represent the most important animal group used in experimental models of liver diseases. The purpose of this study was to determine the reliability of ultrasound findings in the assessment of fatty liver disease and cirrhosis when compared to histological results in Wistar rats by following up a murine model of chronic hepatic disease.ResultsForty Wistar rats (30 treated, 10 controls) were included. Liver injury was induced by dual exposure to CCl4 and ethanol for 4, 8 and 15 weeks. Liver echogenicity, its correlation to the right renal cortex echogenicity, measurement of portal vein diameter (PVD) and the presence of ascites were evaluated and compared to histological findings of hepatic steatosis and cirrhosis. Liver echogenicity correlated to hepatic steatosis when it was greater or equal to the right renal cortex echogenicity, with a sensitivity of 90%, specificity of 100%, positive and negative predictive values of 100% and 76.9% respectively, and accuracy of 92.5%. Findings of heterogeneous liver echogenicity and irregular surface correlated to liver cirrhosis with a sensitivity of 70.6%, specificity of 100%, positive and negative predictive values of 100% and 82.1% respectively, and accuracy of 87.5%. PVD was significantly increased in both steatotic and cirrhotic rats; however, the later had greater diameters. PVD cut-off point separating steatosis from cirrhosis was 2.1 mm (sensitivity of 100% and specificity of 90.5%). One third of cirrhotic rats presented with ascites.ConclusionThe use of ultrasound imaging in the follow-up of murine diffuse liver disease models is feasible and efficient, especially when the studied parameters are used in combination. The potential implication of this study is to provide a non-invasive method that allows follow-up studies of fatty liver disease and cirrhosis of individual rats for pre-clinical drug or cell based therapies.

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Section: Methodsmentioning

confidence: 99%

Ultrasound imaging in an experimental model of fatty liver disease and cirrhosis in rats

et al. 2010

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“…An Ultrasonographic study was designed to check the febrile formation in the wounded liver resulted in liver dysfunction after laparotomy in rats that showed differences in normal liver after laparotomy in rats [26]. A project was formed to check the liver volume measurement has been reported in dogs with CPSS using radiography, ultrasonography, and CT [27].…”

Section: Discussionmentioning

confidence: 99%

Comparative Efficacy of Advanced Surgical Hemostats in Canine Hepatotomy

Abbas¹,

Akbar²,

Khan³

et al. 2018

azb

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In the present study Hemostatic effects of Surgicel Snow and Surgicel Fibrillar in 12 cases in dogs were observed. The dogs were divided into three groups via group A, B and C with four dogs in each group. All three groups were anesthetized by the combination of xylazine (1.1mg/kg) and ketamine (5.5mg/kg) administered intravenous. Group A received Surgical Gauze, Surgicel Snow was employed in Group B and Surgicel Fibrillar was used in Group C. Hemostasis time, Liver function test (LFT), Complete blood count (CBC), Temperature, Pulse rate and Respiratory rate (TPR), Ultrasonography were evaluated. Hemostasis time was significantly less in animals who had received a Surgicel Snow compared to Surgicel Fibrillar and Surgical Gauze. LFT values were significantly different among all three groups. CBC values also differ among all groups. In TPR there were no significant differences in all groups. All groups showed no changes during Ultrasonography of liver. Surgicel Snow is better hemostat than Surgicel Fibrillar with minimum blood loss and least effects on liver function.

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“…Chronic alcohol consumption is a major risk factor for development of hepatic fibrosis [36]. Administration of ethanol with repetitive CCl 4 ingestion could be more effective in maintaining the fibrotic pattern because ethanol increases activation of cytochrome P450 [37], resulting in greater liver damage than with either agent alone [38]. Various plant extracts have been widely applied to treat chronic liver diseases, including chronic hepatic inflammation and fibrosis [39].…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

Mousa¹,

Hassan²,

El-Sammad³

2017

Asian J Pharm Clin Res

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Objective: This study was designed to evaluate the hepatoprotective and antioxidant effects of methanolic extract of Calligonum comosum (C. comosum) shoots on the hepatic fibrosis induced by carbon tetrachloride and ethanol (CCl 4 /ethanol) in rats.Methods: A liver fibrosis was induced in Sprague Dawley rats by oral administration of CCl 4 (1 ml/kg body weight, twice weekly for 10 weeks) along with ethanol (10% in drinking water 1 week before CCl 4 administration and throughout the experiment). Rats were pretreated with C. comosum extract (daily, orally at a dose of 50 mg/kg body weight 1 week before CCl 4 administration). At the end of the experiment, serum, and liver samples were subjected to biochemical investigations. In addition, liver and kidney tissues were evaluated for histopathological changes.Results: C. comosum extract pretreatment significantly reduced CCl 4 -induced elevation in serum levels of aspartate aminotransaminase, alanine aminotransaminase, alkaline phosphatase, total bilirubin, urea, creatinine, and significantly elevated serum contents of total protein and albumin, as well as an improvement in hepatic protein content, albumin/globulin ratio, body weight and relative liver and kidney weights. C. comosum extract also significantly increased the hepatic levels of glucose-6-phosphatase, glutathione peroxidase, glutathione-S-transferase, catalase, superoxide dismutase, and glutathione with significant decrease in the contents of malondialdehyde and protein carbonyl. In addition, downregulation in expression of the fibrotic marker matrix metalloproteinase-2 was observed. C. comosum extract also ameliorated histopathological changes of CCl 4 /ethanol group which further evidenced the biochemical findings. Conclusion:Our results confirmed that the methanolic extract of C. comosum shoots effectively protect against CCl 4 /ethanol-induced liver fibrosis, through its antioxidant property.

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An ultrasound and histomorphological analysis of experimental liver cirrhosis in rats

Cited by 16 publications

References 37 publications

Ultrasound imaging in an experimental model of fatty liver disease and cirrhosis in rats

Ultrasound imaging in an experimental model of fatty liver disease and cirrhosis in rats

Comparative Efficacy of Advanced Surgical Hemostats in Canine Hepatotomy

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

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