Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy

Mbarek, L. Ait; Mouse, Hassan Aît; Jaafari, Abdeslam; Aboufatima, Rachida; Benharref, Ahmed; Kamal, Mehnaz; Bénard, Jean; Abbadi, Najia El; Bensalah, Mohamed Khalil; Gamouh, A.; Chait, Abderrahman; Dalal, A.; Zyad, Abdelmajid

doi:10.1590/s0100-879x2007001100014

Cited by 73 publications

(26 citation statements)

References 30 publications

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“…Cytotoxicity of the EOs could be attributed to their constituents of complex mixtures of monoterpenes and sesqueterpenes as reported in the literature [19]. Antitumor activity of EOs has been reported by various authors, such as the EO of thyme, which contains carvacrol, has a marked in vitro cytotoxic activity against tumor cells [20]. In this study, the EOs displayed the strongest antiproliferative activity was found to show moderate antioxidant activity (unpublished work) suggesting that antioxidant effects have moderate effects on the antiproliferative activity.…”

Section: Resultsmentioning

confidence: 99%

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Al-Zubairi¹,

Al-Mamary²,

Al-Ghasani³

et al. 2017

MOJT

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Essential Oils (EOs) are used in many products to be intended for human utilization. Despite their pharmacological applications in the folk and traditional medicine, studies on EO anti-proliferative properties are still limited. The aim of this study was to investigate the anti-proliferative properties of hydrodistilled EO from 16 aromatic plants grown in Yemen. The cytotoxicity of the EOs was determined against seven cell lines namely; HeLa, MCF7, MDA-MB 231, CEMss, WEHI-3B, 3T3 and CHO cell lines using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay. Lantana camara EO was the most cytotoxic extract against all tested cell lines with an IC50 value of ≤ 0.01 % (v/v), except the non-tumorous cell lines CHO with IC50 value of 0.025 % ± 0.005 % v/v. While Cinnamomum zeylanicum EO showed high cytotoxic activity against HeLa, MDA-MB 231, 3T3 and CHO cell lines with IC50 value of ≤ 0.010 % (v/v). EO from Ocimum basilicum and Mentha piperita were among the ones with no activity or IC50 value of > 0. 010 % (v/v). The results demonstrated the potential of the EO from aromatic plants from Yemen for possible source of cancer treatment. type apparatus. The oil layer was collected, however, in some cases the distillate aqueous layers were washed with ether to extract any dissolved oils in water. Then, the ether was separated by separatory funnel and evaporated on water bath at 40 °C and the residue EO was added to the first collected portion. The EO was dried over anhydrous sodium sulfate and the yield was calculated. Keyword: Cell cultures and maintenanceHuman cervical cancer cells (HeLa), human breast cancer cell lines (MCF-7), human mammary cancer cell lines-estrogen negative (MDA-MB-231), human colon carcinoma cell lines (HT-29), mouse fibroblast cell lines (3T3) and murine monomyelocytic leukemia cell lines (WEHI-3) were obtained from ATTC. While Chinese hamster ovary cell line (CHO) from ECACC and T4-lymphoplastoid cell lines (CEMss) from NIH (AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: USA). Cell lines were grown in RPMI 1640 supplemented with 10% fetal calf serum, 1% penicillin-streptomycin and 1% amphotericin B. Flasks containing cell lines were incubated in a humidified incubator with 5% CO 2 , at 37 °C. Cultures were frequently examined under inverted microscope (Micros, Austria). Once cells reached 80% confluency, media was removed and the cells were washed 3 times with 7mL of PBS (Phosphate Buffer Saline). Two milliliters of trypsin was added to the adherent cells and were incubated for 5 minutes. The flask was tapped gently to detach cells from the wall of the flask to appear as single cells. Ten milliliters of RPMI 1640 with 10% FCS were added to the flask and the content of the flask was resuspended to allow cells to disperse. About 6mL of cell suspension was transferred into a 75cm3 flask. Ten milliliters of RPMI 1640 with 10% FCS were then added and incubated in CO 2 incubator at 37 °C. The cells were frequently examined under an inverted microsco...

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Section: Resultsmentioning

confidence: 99%

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Al-Zubairi¹,

Al-Mamary²,

Al-Ghasani³

et al. 2017

MOJT

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“…Adenosine triphosphate cassette (ABC)-transporter family proteins confer MDR due to their increased activity [95]. EOs can circumvent the reluctance of tumours to respond to the cytotoxic drugs [96]. EOs of thyme are effective against widely used drugs like Adriamycin, Vincristine, and Cisplatin resistant ovarian cancer cell lines and, in addition, tumour size reduction was also observed in vivo which indicates the efficacy of the EO in mammalian system [96, 97].…”

Section: Multidrug Resistance (Mdr) In Cancer: a Potential Set Backmentioning

confidence: 99%

“…EOs can circumvent the reluctance of tumours to respond to the cytotoxic drugs [96]. EOs of thyme are effective against widely used drugs like Adriamycin, Vincristine, and Cisplatin resistant ovarian cancer cell lines and, in addition, tumour size reduction was also observed in vivo which indicates the efficacy of the EO in mammalian system [96, 97]. Juniperus excels EO was effective against MDR P-glycoprotein-expressing CEM/ADR5000 leukemia cells and reversed their resistance indicating the use of EO in MDR treatment in cancer [98].…”

Section: Multidrug Resistance (Mdr) In Cancer: a Potential Set Backmentioning

confidence: 99%

Essential Oils and Their Constituents as Anticancer Agents: A Mechanistic View

Gautam

Mantha

Mittal

2014

BioMed Research International

213

137

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Exploring natural plant products as an option to find new chemical entities as anticancer agents is one of the fastest growing areas of research. Recently, in the last decade, essential oils (EOs) have been under study for their use in cancer therapy and the present review is an attempt to collect and document the available studies indicating EOs and their constituents as anticancer agents. This review enlists nearly 130 studies of EOs from various plant species and their constituents that have been studied so far for their anticancer potential and these studies have been classified as in vitro and in vivo studies for EOs and their constituents. This review also highlights in-depth various mechanisms of action of different EOs and their constituents reported in the treatment strategies for different types of cancer. The current review indicates that EOs and their constituents act by multiple pathways and mechanisms involving apoptosis, cell cycle arrest, antimetastatic and antiangiogenic, increased levels of reactive oxygen and nitrogen species (ROS/RNS), DNA repair modulation, and others to demonstrate their antiproliferative activity in the cancer cell. The effect of EOs and their constituents on tumour suppressor proteins (p53 and Akt), transcription factors (NF-κB and AP-1), MAPK-pathway, and detoxification enzymes like SOD, catalase, glutathione peroxidase, and glutathione reductase has also been discussed.

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“…Our results revealed that thyme oil had an anti-proliferative effect in PC3 and HCT116 cell lines closed to the doxorubicin; it was more effective than the doxorubicin in MCF-7 and A549 cell lines. According to AitM'barek et al (2007), the thyme oil, which contains thymol as its major constituent, has an important in vitro cytotoxic activity against tumor cells. Our thyme EO inhibited the viability of several tumor cell lines.…”

Section: In Vitro Antiproliferative and Cytotoxic Activitymentioning

confidence: 99%

Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

Ramadan¹,

Ali²,

Ghanem³

et al. 2015

Grasas y Aceites

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SUMMARY:Inhibitors of tumor growth using extracts from aromatic plants are rapidly emerging as important new drug candidates for cancer therapy. The cytotoxicity and in vitro anticancer evaluation of the essential oils from thyme, juniper and clove has been assessed against five different human cancer cell lines (liver HepG2, breast MCF-7, prostate PC3, colon HCT116 and lung A549). A GC/MS analysis revealed that α-pinene, thymol and eugenol are the major components of Egyptian juniper, thyme and clove oils with concentrations of 31.19%, 79.15% and 82.71%, respectively. Strong antioxidant profiles of all the oils are revealed in vitro by DPPH and β-carotene bleaching assays. The results showed that clove oil was similarly potent to the reference drug, doxorubicin in prostate, colon and lung cell lines. Thyme oil was more effective than the doxorubicin in breast and lung cell lines while juniper oil was more effective than the doxorubicin in all the tested cancer cell lines except prostate cancer. In conclusion, the essential oils from Egyptian aromatic plants can be used as good candidates for novel therapeutic strategies for cancer as they possess significant anticancer activity. KEYWORDS:Anticancer; Antioxidant; Clove Juniper; Thyme RESUMEN: Aceites esenciales de plantas aromáticas egipcias como novedosos agentes anticancerígenos y antioxidantes en líneas celulares de cáncer humano. Los inhibidores de crecimiento de tumores usando extractos de plantas aromáticas están emergiendo con rapidez como nuevos e importantes medicamentos para el tratamiento del cáncer. La citotoxicidad y la acción anticancerígena in vitro de aceites esenciales de tomillo, enebro y clavo han sido evaluadas en cinco líneas celulares de cáncer humano (hígado HepG2, mama MCF-7, próstata PC3, colon HCT116 y pulmón A549). Los análisis de GC/MS mostraron que α-pineno, timol y eugenol son los principales componentes de los aceites egipcios de enebro, tomillo y clavo, con concentraciones de 31,19%, 79,15% y 82,71%, respectivamente. Se demuestra, mediante ensayos in vitro de blanqueo de DPPH y β-caroteno, el enérgico perfil antioxidante de todos los aceites. Los resultados mostraron que el aceite de clavo fue similar de potente al fármaco de referencia, doxorrubicina en las líneas celulares de próstata, colon y pulmón. El aceite de tomillo fue más efectivo que la doxorrubicina en las líneas celulares de mama y de pulmón, mientras que el aceite de enebro fue más eficaz que la doxorrubicina en todas las líneas celulares de cáncer ensayados, excepto en la de cáncer de próstata. En conclusión, los aceites esenciales de plantas aromáticas egipcias se pueden utilizar como buenos candidatos para nuevas estrategias terapéuticas para el cáncer al poseer una significativa actividad anticancerígena

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Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy

Cited by 73 publications

References 30 publications

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Essential Oils and Their Constituents as Anticancer Agents: A Mechanistic View

Essential oils from Egyptian aromatic plants as antioxidant and novel anticancer agents in human cancer cell lines

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