Pattern of Wnt ligand expression during chick eye development

Rossi, Eliane; Siwiec, Fernanda; Yan, Chao

doi:10.1590/s0100-879x2006005000155

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…These results point to the difficulty in studying Wnt ligands during development: they are often expressed in highly redundant, overlapping patterns. Other Wnts have been identified in the RPE of mouse and chick including Wnt5a and Wnt5b (Fokina and Frolova, 2006; Rossi et al, 2007; Van Raay and Vetter, 2004). Further work is necessary to identify other members of the Wnt family that are expressed in the RPE and their individual contributions to D-V retinal patterning.…”

Section: Discussionmentioning

confidence: 99%

Canonical Wnt signaling is required for the maintenance of dorsal retinal identity

et al. 2008

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Accurate retinotectal axon pathfinding depends upon the correct establishment of dorsal-ventral retinal polarity. We show that dorsal retinal gene expression is regulated by Wnt signaling in the dorsal retinal pigment epithelium (RPE). We find that a Wnt reporter transgene and Wnt pathway components are expressed in the dorsal RPE beginning at 14-16 hours post-fertilization. In the absence of Wnt signaling, tbx5 and Bmp genes initiate normal dorsal retinal expression but are not maintained. The expression of these genes is rescued by the downstream activation of Wnt signaling, and tbx5 is rescued by Bmp signaling. Furthermore, activation of Wnt signaling cannot rescue tbx5 in the absence of Bmp signaling, suggesting that Wnt signaling maintains dorsal retinal gene expression by regulating Bmp signaling. We present a model in which dorsal RPE-derived Wnt activity maintains the expression of Bmp ligands in the dorsal retina, thus coordinating the patterning of these two ocular tissues.

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Section: Discussionmentioning

confidence: 99%

Canonical Wnt signaling is required for the maintenance of dorsal retinal identity

et al. 2008

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“…The Wnt signalling pathway directs dorsal cell fate decisions during embryonic development (reviewed by Klaus and Birchmeier, 2008). Therefore, we next studied the expression patterns of various Wnt family members, such as Wnt2b, Wnt3a and Wnt8b (see also GarciaLopez et al, 2004;Rossi et al, 2007;Quinlan et al, 2009;Van Raay and Vetter, 2004). Of the analysed Wnt ligands, only Wnt2b transcripts were restricted to the dorsal surface ectoderm and dorsal ov at the time the RPE is specified (Fig.…”

Section: Molecular Signals Involved In Optic Vesicle Patterningmentioning

confidence: 99%

RPE specification in the chick is mediated by surface ectoderm-derived BMP and Wnt signalling

Steinfeld

Coronato

et al. 2013

Development

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The retinal pigment epithelium (RPE) is indispensable for vertebrate eye development and vision. In the classical model of optic vesicle patterning, the surface ectoderm produces fibroblast growth factors (FGFs) that specify the neural retina (NR) distally, whereas TGFβ family members released from the proximal mesenchyme are involved in RPE specification. However, we previously proposed that bone morphogenetic proteins (BMPs) released from the surface ectoderm are essential for RPE specification in chick. We now show that the BMP-and Wnt-expressing surface ectoderm is required for RPE specification. We reveal that Wnt signalling from the overlying surface ectoderm is involved in restricting BMP-mediated RPE specification to the dorsal optic vesicle. Wnt2b is expressed in the dorsal surface ectoderm and subsequently in dorsal optic vesicle cells. Activation of Wnt signalling by implanting Wnt3a-soaked beads or inhibiting GSK3β at optic vesicle stages inhibits NR development and converts the entire optic vesicle into RPE. Surface ectoderm removal at early optic vesicle stages or inhibition of Wnt, but not Wnt/β-catenin, signalling prevents pigmentation and downregulates the RPE regulatory gene Mitf. Activation of BMP or Wnt signalling can replace the surface ectoderm to rescue MITF expression and optic cup formation. We provide evidence that BMPs and Wnts cooperate via a GSK3β-dependent but β-catenin-independent pathway at the level of pSmad to ensure RPE specification in dorsal optic vesicle cells. We propose a new dorsoventral model of optic vesicle patterning, whereby initially surface ectoderm-derived Wnt signalling directs dorsal optic vesicle cells to develop into RPE through a stabilising effect of BMP signalling.

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“…Multiple lines of evidence implicate several Frizzled family members, including FZD 1, 2, 3, 6, 8, and 10, in mediating Wnt signaling in the dorsal spinal cord; however, the role of FZD10 requires clarification (Borello et al, 1999;Bourhis et al, 2010;Cauthen et al, 2001;Chesnutt et al, 2004;Deardorff et al, 2001;Fokina and Frolova, 2006;Garcia-Morales et al, 2009;Hollyday et al, 1995;Kawakami et al, 2000a;McCabe et al, 2007;Nunnally and Parr, 2004;Paxton et al, 2010;Rossi et al, 2007;Stenman et al, 2008;Summerhurst et al, 2008;Wang et al, 2006;Yan et al, 2009;Yu et al, 2010). Previous reports suggest that FZD10 synergizes with WNT1, but not WNT3A, in an axis duplication assay in Xenopus (Garcia- Morales et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo

Galli

Munji

Chapman

et al. 2014

Developmental Dynamics

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Background WNT1 and WNT3A drive a dorsal to ventral gradient of β-catenin dependent Wnt signaling in the developing spinal cord. However, the identity of the receptors mediating downstream functions remains poorly understood. Results In this paper, we show that the spatiotemporal expression patterns of FZD10 and WNT1/WNT3A are highly correlated. We further show that in the presence of LRP6, FZD10 promotes WNT1 and WNT3A signaling using an 8xSuperTopFlash reporter assay. Consistent with a functional role for FZD10, we demonstrate that FZD10 is required for proliferation in the spinal cord. Finally, by using an in situ proximity ligation assay, we observe an interaction between FZD10 and WNT1 and WNT3A proteins. Conclusion Together, our results identify FZD10 as a receptor for WNT1 and WNT3A in the developing chick spinal cord.

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Pattern of Wnt ligand expression during chick eye development

Cited by 6 publications

References 18 publications

Canonical Wnt signaling is required for the maintenance of dorsal retinal identity

Canonical Wnt signaling is required for the maintenance of dorsal retinal identity

RPE specification in the chick is mediated by surface ectoderm-derived BMP and Wnt signalling

Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo

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