2006
DOI: 10.1590/s0100-879x2006000600007
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Abstract: Invasive bacteria can induce their own uptake and specify their intracellular localization; hence it is commonly assumed that proximate modulation of host cell transcription is not required for infection. However, bacteria can also modulate, directly or indirectly, the transcription of many host cell genes, whose role in the infection may be difficult to determine by global gene expression. Is the host cell nucleus proximately required for intracellular infection and, if so, for which pathogens and at what sta… Show more

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Cited by 7 publications
(16 citation statements)
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“…Infection with the facultative intracellular bacterium Shigella flexneri, however, was significantly reduced in L929 cytoplasts, and as in the present results, the maximal bacterial loads attained were diminished in comparison to those in nucleated cells (54). It was also reported, on the basis of few observations, that T. cruzi amastigotes did not differentiate and replicate in HeLa cell cytoplasts (28).…”
Section: Discussionsupporting
confidence: 79%
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“…Infection with the facultative intracellular bacterium Shigella flexneri, however, was significantly reduced in L929 cytoplasts, and as in the present results, the maximal bacterial loads attained were diminished in comparison to those in nucleated cells (54). It was also reported, on the basis of few observations, that T. cruzi amastigotes did not differentiate and replicate in HeLa cell cytoplasts (28).…”
Section: Discussionsupporting
confidence: 79%
“…Cells were grown in complete Dulbecco's modified Eagle's medium (cDMEM) with 5% fetal bovine serum, 2 g/liter bicarbonate, and 15 mM HEPES. Confluent cultures were trypsinized, and 1 ϫ 10 5 cells were seeded for 24 h in 2-cm 2 wells, each containing an 8-by 12-mm "minislide" cut from a standard microscope slide (54). Vero cells (African green monkey kidney fibroblasts) and CHO-K1 cells (Chinese hamster ovary cell line) were grown at 37°C in RPMI 1640 medium supplemented with 5% fetal bovine serum, streptomycin (100 g/ml), and penicillin (100 U/ml).…”
Section: Methodsmentioning
confidence: 99%
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“…We probed this hypothesis by infecting enucleated cells (cytoplasts) obtained by centrifugation, with T. gondii . Host cell enucleation results in the abrupt interruption of ongoing nuclear gene transcription, nuclear mRNA processing and nucleus‐dependent signalling cascades (Yamamoto et al ., 2006). Hence, cytoplasts are a relevant model to evaluate the requirement of host nuclear activities for parasite invasion, replication and PV modifications post entry.…”
Section: Introductionmentioning
confidence: 99%